From 120 soil and activated sludge, the strains able to grow on Benzoate and m-Toluate have been isolated after selective enrichment which were later identified as Psudomonas sp. according to its morphological and biochemical characteristics. Ben-2 strain contained two plasmid DNA having about 120 Kb and below 2.0 Kb by agarose gel electrophoresis. Form the comparative investigation of catechol 1,2-oxygenase and catechol 2,3-oxygenase activities in Ben-2 strain and its cured strain, Ben-2 strain has both of these two enzymes while cured strain has catechol 1,2-oxygenase only.

• 정준영(충북대학교 식품공학과, Department of Food Science and Technology, College of Agriculture. Chungbuk National Uni‘versity) | Jun-Young Jeong
• 김교창(충북대학교 식품공학과, Department of Food Science and Technology, College of Agriculture. Chungbuk National Uni‘versity) | Kyo-Chang Kim