This study was to investigate time-dependent degradation process under various pH condition for organophosphorus(org-p) insecticides, namely Demeton-s-methyl, Diazinon, Parathion, Phenthoate, and EPN in several waters. They were analysed by GC-FTD according to standard methods for the examination of water and wastewater. In pH=4, diazinon showed disappearance after 14 days in chromatogram. In pH=11, org-p insecticides were almost degradable after 7 days. In this condition, effect of pH on degradation process was greater than that of light. In pH=7, org-p insecticides persisted residues after 112 days except Demeton-s-methyl. In BOD_5 120, 250 ppm and domestic water, org-p insecticides showed also rapid degradation process.
The major purpose of the present study is to survey vegetables, meats, seafoods, processed foods and imported foods for the presence. of Listeria spp. and to prevent listeriosis caused by the contamination of Listeria monocytogenes. Listeria spp. was isolated from 6.0% of meats, 3.4% of seafoods, and 11.4% of imported foods but was not found in vegetables and processed foods. The optimum growth condition of isolates indentified as Listeria monocytogenes was pH 7.0 and 37℃. The antimicrobial effect of grapefruit seed e×tract (GFSE) was observed in the level of more than 100ppm by disk method. When lml(2.5× 10^6 CFU/ml) of Listeria monocytogenes was inoculated and incubated for 3 days at 30, the total cell number of the organism was 4.5 × 10^9 in the control, 7.2 × 10³ in 100 μg/ml of GFSE medium, and 3.5 μg/ml of GFSE medium. Direct visualization of microbial cells by using both transmission electron microscope and scanning electron microscope showed microbial cell membrane the function of which was destroyed by treating with the dilute solutions of GFSE. It could be confirmed that GFSE completely inhibited the growth of the test strain of Listeria monocytogenes.
The oxidation of cholesterol in tallow heated at three frying temperatures, 130, 150 and 180℃ was studied by assaying cholesterol oxidation products(COP) by GC-MS. The correlation between levels of COP and changes of physicochemical parameters (peroxide value, polymer, polar components and dielectric constant) in tallow heated were studied. As temperature increased, the amount of cholesterol was decreased proportionally with heating time. However, the levels of COP did not increase considerably with increased frying temperature. The rate of cholesterol disappearance was the greatest at 180℃ and the smallest at 130℃. Larger amounts of COP formed were found at 150℃ than at 180℃. The levels of COP formed in tallow heated showed highly correlation with(r=0.94, n=30, p$lt;0.01) polymer, polar components and dielectric constant, respectively.
From 120 soil and activated sludge, the strains able to grow on Benzoate and m-Toluate have been isolated after selective enrichment which were later identified as Psudomonas sp. according to its morphological and biochemical characteristics. Ben-2 strain contained two plasmid DNA having about 120 Kb and below 2.0 Kb by agarose gel electrophoresis. Form the comparative investigation of catechol 1,2-oxygenase and catechol 2,3-oxygenase activities in Ben-2 strain and its cured strain, Ben-2 strain has both of these two enzymes while cured strain has catechol 1,2-oxygenase only.
Customary usage of oil at homes and rancidity of edible soybean oil by cooking frequency at homes and mass meal services were investigated. 80% of house wives bought the cooking oil by 1.8 f unit container and 70% of them read either the manufactured date or explanatory note for use. 85% of house wives kept oil in the storage case under sink or in the pantry chest, and 80% of oils were used once or twice and 20% used three times for cooking. Acid value(AV), iodine value(IV), peroxide value(POV), carbonyl value(CoV) and thiobarbituric acid value(TBAV) of fresh soybean oil were lower than standard level. In the rancidity by cooking frequency, the acid value, peroxide value, carbonyl value and thiobarbituric acid value increased significantly when oil was used once and iodine value decreased significantly when used once and twice at both mass meal services and homes. The level of the acid value, iodine value, carbonyl value and thiobarbituric acid value of oil used at mass meal services did not show significant difference from those of oil used at homes. But, the peroxide value of oil used thrice at mass meal services was significantly higher than those of homes.
본 연구에서는 재래 방법에 따라 생산되고 있는 전통발효식품(된장, 간장, 고추장)에 함유되어 있는 Ochratoxin A(OT-A) 를 면역학적 정량분석법인 Enzyme-Linked Immunosorbent Assay (ELl SA)와 Chemiluminescence Immunoassay(CIA)법을 개발하여 훈석하였다. 각 가정에서 생산하여 소비되는 장류(된장 13종. 간장 12종. 고추장 14종)와 채래척언 방법으로 생산하여 국 내시장에서 유통되고 있는 장류(된장 17종, 간장 11총)로 나누어 분석올 실시하였다.OT-A 흘 정량 조사한 표준독션의 작성 결과 CIA나 ELISA 모두 sensiti까ty는 20 pg/assay 이었으며, 본 실험에서 사용한 면역분석법의 OT-A 회수율은 90% 이상이었다.OT-A 의 잔존량은 가정에서 생산하여 소비되는 시료의 경우 된장 7.1 :t 3.7 ng/g, 간장 2. l:t 2.6 ng/g 그리고 고추장이 4.0 :t 1.9 ng/g 이었으며, 반면 재래시장에 유통되고 있는 시료의 경우 된장이 비교척 잔존량이 높은 22.5 :t 14.0 ng/g, 간장이 16.9:t 4.1 ng/g으로 나타나 가정에서 생산되고 있는 전통발효식품의 경우 OT-A의 오염도가 아주 낮은 것으로 나타났다. 한편 OT-A 의 가옐안정생 시험에서는 60, 90, 121 "c 에서 120 분까지 가열 처리하여 OT-A의 잔존량올 조사하였던 바 121 "c 의 고압처리(1 20 min)에서도 안정하였다.