간행물

한국식품위생안전성학회지 KCI 등재 Journal of Food Hygiene and Safety

권호리스트/논문검색
이 간행물 논문 검색

권호

Vol.14 No.3 (1999년 9월) 14

1.
1999.09 구독 인증기관 무료, 개인회원 유료
A total of 136 coliform bacteria isolated from spring water (112 strains) and ground water (24 strains), submitted to Seoul Health and Environmental Research Institute from June to July in 1997, were characterized biochemically and microbiologically. Colonical characteristics of each isolate were also noted, including color and texture on EMB agar. Among the 136 isolates, 50.7% were greenish metallic sheen color, 44.2% were pink and 5.1% were violet. The sixty four percent were smooth, 34.6% were mucoid and 0.7%. were rough. Twenty three bacterial species were identified by IMViC and API 20E test. Among the 136 coliform bacteria known to species, 39 isolates (28.6%) were Escherichia spp., 32 isolates (23.5%) were Klebsiella ssp., 30 isolates (22.1%) were Enterobacter spp., 19 isolates (14.0%) were Serratia spp., 6 isolates (4.4%) were Citrobacter spp., 4 isolates (3.0%) were Kluyuera spp. and 7 isolates (5.1%) were other bacterial species. Strains, which were gas-positive in lactose broth but gas-negative in Kligler Iron Agar were Ent. intermedium, Ser. liquefaciens, Ser. marcescenes and Salmonella arizoae. Strains, which were H2S production were also Kleb. pneumoniae, Kleb. oxytoca, Kleb. ornithinolytica, Ent. sakazakii, Ent. cloacae, Ser. liquefaciens, Ser. ficaria, Cit. freundii and Sal. arizoae. In the present study, most of coliform isolated from spring and ground water were E. coli, Klebsiella spp. and Enterobacter spp. Since coliform with pink colony in EMB agar was isolated as frequent as coliform with greenish metallic sheen colony, coliform with pink colony should be considered as important colony. Our results suggested that new coliform strains may be emerging on the basis of biochemical and microbiological testes.
4,000원
2.
1999.09 구독 인증기관 무료, 개인회원 유료
The bacteriocin produced by Lactobacillus sp. GM7311 was purified by sequential steps including n-propanol/acetone treatment, CM-cellulose chromatography, and gel filtration on Sephacryl HR-100. The relative activity of bacteriocin increased 493-fold after final purification step with a recovery of 8.3%. Two protein bands of ca. 8,200 and 2,500 were detected by SDS-PAGE of bacteriocin purified through CM-cellulose and sephacryl HR-100 chromatography and both of them had bacteriocin activity.
4,000원
3.
1999.09 구독 인증기관 무료, 개인회원 유료
The storage stability from powdered anchovy and its preparation cooperated with three additives (water extract from green tea and boxthorn and BHT) was studied. The results are as follows: 1. When the additives were added to powdered anchovy, the storage stability was improved. The antioxidative activities of water extract from green tea and boxthorn were considerably higher than BHT. The antioxidative activities were decreased in the rank order boxthorn $gt; green tea $gt; BHT. 2. The more the concentration of natural additives was increased, the more the storage stability was improved. The rank order was 60° $gt; 40° $gt; 20° Bx. 3. The AV and POV of powdered anchovy were increased in the rank order to 6 $lt; 12 $lt; 18 $lt; 24 $lt; 30 months by storage term. 4. This clearly suggested that water extract at 20° Bx level from boxthorn was possible utilization as a new natural antioxidant for storage stability of powdered anchovy.
4,000원
4.
1999.09 구독 인증기관 무료, 개인회원 유료
This study was carried out to determine the contents of sodium saccharin, sodium benzoate and potassium sorbate in danmooji, jangachi, puffed cereal, mixed beverages, carbonated beverages, fruit and vegertable juices (excepted unheated fruit and vegertable juices) and dried fishery products(seasoned) in the market. Sodium saccharin, sodium benzoate and potassium sorbate were analyzed by HPLC at the wavelength of 215 nm with 0.05% ammonium phosphate dibasic acetonitril mobil phase(94 : 6), and recovery rates were 96.2-100.3%, 95.8-100.9%, 96.1-99.8%, respectively. The contents of sodium saccharin were N.D.-1234.8 mg/kg in danmoogi, jangachi, puffed cereal and mixed beverages and sodium benzoate in mixed beverages, carbonated beverages, fruit and vegertable juices were N.D.-663.2 mg/kg, and potassium sorbate in danmooji, jangachi and dried fishery products were N.D.-2725.2 mg/kg.
4,000원
5.
1999.09 구독 인증기관 무료, 개인회원 유료
The present study was performed to investigate biodegradation rate of BPMC(2-sec-butylphenyl methyl carbamate) and chlorothalonil. In the biodegradation test of two pesticides by the modified river die-away method from June 17 to August 22, 1998, the biodegradation rate constants and half-life were determined in Nakdong(A) and Kumho River(B). Bio- degradation rate of BPMC was 27% in A sampling point, 40% in B sampling point after 7 days. Biodegradation rate constants and half-life of BPMC were 0.0460 and 15.1 days in A sampling point, 0.0749 and 9.3 days in B sampling point, respectively. Biodegradation rate of chlorothalonil was 100% in A and B sampling points after 7 days. Biodegradation rate constants and half-life of chlorothalonil were 0.1416 and 4.9 hours in A sampling point, 0.1803 and 3.8 hours in B sampling point, respectively. Biodegradation rate of chlorothalonil was faster than that of BPMC. Correlation analysis between biodegradation rate constants of pesticides and water quality(DO, BOD, SS, ABS, NH₃-N and NO₃-N) showed significant correlation with BOD, SS and NH,-N. Furthermore, regression analysis with BOD, SS and NH₃-N as independent variable and biodegradation rate constant as independent variable showed a significant linear equation. These results suggested that BPMC and chlorothalonil were mainly degraded by biodegradation, and the difference in biodegradation of two pesticides was due to difference of water quality.
4,000원
6.
1999.09 구독 인증기관 무료, 개인회원 유료
The acute toxicity of xylooligosaccharide(XO) was evaluated in SD rats. Groups of 15 male and 15 female rats were orally administered XO (0, 5000 or 10000 ㎎/㎏). The changes of body weight and clinical signs were investigated for 14 days after treatments. No death and toxic effects were observed for 14 days. Soft stool and diarrhea appeared right after treatment for over dose and non-digestive feature of XO but these clinical signs disappeared on the next day. No significant changes in body weight and abnormal gross findings were observed in relation to XO. According to the results, XO has no special toxic effects and LD50 values of XO are above 10000 ㎎/㎏ in male and female rats.
3,000원
7.
1999.09 구독 인증기관 무료, 개인회원 유료
To evaluate the bacterial reverse mutation of xylooligosaccharide(XO)s the in vitro Ames test using Salmonella typhimurium (TA98, TA100, TA1535, TA1537) and Escherichia coli (WP2 uvrA) was performed. XO was negative in Ames test with Salmonella typhimurium and Escherichia coli with and without rat liver microsomal enzyme (S-9 fraction). According to the results, XO does not cause bacterial reverse mutation.
4,000원
8.
1999.09 구독 인증기관 무료, 개인회원 유료
Paralytic shellfish poison threatens public health most seriously from April to early May every year and gives adverse effects on export of these products. Major shellfish products exported were canned oyster, Crassostrea gigas and blue mussel, Mytilus edulis. Toxicities of canned shellfishes with toxin of low levels were mostly inactivated during the processing; in contrast, residual toxicity was of great concern with canned products from highly toxic shellfishes. This study was to provide basic data to establish food safety measure by evaluating the changes of toxicity during 2 year storage of canned products with toxic blue mussel and oyster. Any significant difference was not observed between two samples. Boiled can and smoked can showed inactivation of toxicity to some extent, whereas acidified can did not show reduction of toxicity even after 2 year storage. In case the initial toxicity of shellfish was high long term storage could not inactivate the toxicity of the canned product.
4,000원
9.
1999.09 구독 인증기관 무료, 개인회원 유료
In previous reports, the authors isolated the algicidal marine bacterium, Alteromonas sp. SR-14 and demonstrated its growth inhibition of diatom, Chaetoceros calcitrans (C. calcitrans). In this paper, we studied the effects of cell free culture filtrate of Alteromonas sp. SR-14 on the growth of C. calcitrans, and the characteristics of the algal growth inhibition substance. The culture filtrate of Alteromonas sp. SR-14 grown in peptone broth showed growth inhibition activity against C. calcitrans. The reasonable culture conditions of the bacterium for producing of algal growth inhibition substances were 15-20℃ in temperature, 7.0-9.0 in pH and 23-30‰ in salinity, respectively. The algal growth inhibition activity of culture filtrate was increased from stationary phase in growth curve of Alteromonas sp. SR-14. The molecular weights of algal growth inhibition substances produced by Alteromonas sp. SR-14 were ranged about from 3 KDa to 12 KDa. Among the substances, less than 10 KDa fraction were stable by heating at 100℃ for 10 minutes, while more than 10 KDa fraction were heat labile. According to the experimental results, the algal growth inhibition substance produced by the bacterium was not a single compound.
4,000원
10.
1999.09 구독 인증기관 무료, 개인회원 유료
Chemical composition of imported table salts from China (sun dried), Thailand (sun dried) and Australia (rock salt) were compared the mineral content and heavy metal contamination with Korean sun dried salt. Kimchies prepared by those salts were analyzed the quality by pH, acidity and sensory evaluation. Chemical compositions were as follows : moisture-from 1.7% (Australia) to 8.7% (Korea), sodium chloride-from 86.7% (China, summer) to 97% (Australia), insoluble matter in water-from 0.02% (Australia) to 0.18 (China), and sulfate ion-from 0.03% (Australia) to 1.65% (China, summer). Judging by Korean Standard(KS) of salt, salt from Australia and Thailand belong to lst grade, Korean and Chinese (winter) to 2nd grade, and China (summer) were out of 3rd grade. Ca and Mg content of the salts tested were 0.022% (Australia) to 0.246 (China, winter), 0.036% (Australia) to 0.$41% (China, summer) respectively and Pb was 1.23 ppm(China, summer)-0.61ppm(Korea) which is within the Korean regulation. The other heavy metals, Pb, Cd, As and Hg were not detected in all salts tested. The quality of Kimchi prepared by those salts were significantly no difference with Korean sun dried salt.
4,000원
11.
1999.09 구독 인증기관 무료, 개인회원 유료
Effect of quinolinedione derivative (OQ-21) on phenylephrine induced vasoconstriction was investigated using aortic rings in organ bath isolated from rats. Treatment with OQ-21 resulted in moderate increase in vasoconstriction in a dose-dependent manner. In addition we studied acute intraperitoneal toxicity of OQ-21 in male and female ICR mice. The changes of body weight and clinical signs were observed for 7 days after single dose of OQ-21 from 50 mg/kg to 500 mg/kg. There were no significant changes in body weight and clinical signs. Any mouse didn't die even at maximal dose. Autopsy of OQ-21 treated mice revealed no abnormal difference from contol mice, These results suggest that OQ-21 be moderately safe and could be developed as effective drug.
4,000원
12.
1999.09 구독 인증기관 무료, 개인회원 유료
Every yaar approximately 12,000 people die from severe burns and thermal injury. Distinguishing a minor burn from a more serious burn involves determining the degree of damage to the tissues of the body. The major lethal problems are massive fluid losses and microbial infections. Xanthan gum and algin are known as natural polymers. we prepared the official burn ointment which were made by xanthan gum and algin. This burn ointment was covered on the skin wound of artificial burned and their effect of healing were investigated by the evaluation of histological and hematological change as a function of time. The result of rats test showed that burn ointments made from xanthan gum and algin were more effective in formation of the new tissue and reduction of inflammation than the controls. From the results obtained in this study, natural polymer ointment which were prepared from xanthan gum and algin were found to satisfy most of the existing ointment substitute.
4,000원
13.
1999.09 구독 인증기관 무료, 개인회원 유료
The purpose of this study was to evaluate the microbiological quality, and to assure the hygienic safety of the food production in the university foodservice facility located in Seoul in accordance with the Hazard Analysis Critical Control Point(HACCP) concepts. In the hygienic state assessment of kitchen, it has revealed that it was very important to remove water from the kitchen floor and to establish standard method for disinfection of cooking utensils. And foodservice workers were required to have training program for the safe handling of food and utensils since they did treat food without hygienic gloves. The kitchen layout had to be improved because the near distance of table with heating unit and shelf might cause the growth of microorganisms when prepared food was kept on the shelf. In terms of the time-temperature measurement and microbiological quality assessment during each of the food production phases, most of sengchae (raw vegetable dish) and namul (cooked vegetable dish) were treated within danger zone for food safeness (5-60℃). It has shown that the microbiological quality of raw materials was very much inferior at the time of receiving based on the TPC(10^5-10^7), coliform(10³-10^6), which was not acceptable level(TPC:10^6, coliform:l0³) suggested by Solberg. Microbiological growth has increased in the both of sengchae and namul considerably during most of food production phase. Therefore, it is extremely important to reduce holding and serving time and to avoid treating food within the danger zone for food safeness. In addition, the prevention of cross-contamination during mixing the ingredients with improper equipments and with insanitary treatments by workers was also important to keep the food safety in this speciqic university foodservice facility.
4,300원
14.
1999.09 구독 인증기관 무료, 개인회원 유료
This study were conducted to estimate the values of the heavy metal in the fresh water fish and shellfish collected from fish market. The levels of the heavy metal were determined in a total of 319 samples of 11 species of the fresh water fish and 14 species of the shellfish by mercury analyzer and atomic absorption spectrophotometer. The results of this study were summerized as follows; The average contents of the heavy metal in the fresh water fish were Pb 0.075, Hg 0.053, Cd 0.002, Cr 0.135, Fe 8.695, Mn 1.078, Zn 9.491, Cu 0.548 mg/ kg. The average contents of the heavy metal in the shellfish were Pb 0.059, Hg 0.007, Cd 0.146, Cr 0.147, Fe 40.808, Mn 7.738, Zn 13.943, Cu 2.731 mg/kg. Being compared the average contents of the heavy metal of the fresh water fish with those of shellfish, the average contents of Cd, Fe, Mn, Cu in the shellfish were significantly higher than in the fresh water fish(P$lt;0.001). Depending on the sampling areas, the average contents of the heavy metal were different by districts. The contents of the heavy metal were changed with the seasons(P$lt;0.05).
4,600원