The objective of this study is investigation of contamination levels and assessment of health risk effects of heavy metals in herbal pills. 31 Items and 93 samples were obtained for this investigation from major herbal medicine producing areas, herbal markets and on-line supermarkets from Jan to Jun in 2010. Inductively coupled plasma mass spectrometer method was conducted for the quantitative analysis of Pb, Cd and As. In addition, the mercury analyzer system was conducted for that of Hg without sample digestion. The average contents of heavy metals in samples were as follows : 0.87 mg/kg for Pb, 0.08 mg/kg for Cd, 2.87 mg/kg for As and 0.16 mg/kg for Hg, respectively. In addition, the average contents of heavy metals in different parts of plants, including cortex, fructus, herba, radix, seed, algae and others were 0.63 mg/kg, 3.94 mg/kg, 1.42 mg/kg, 1.05 mg/kg, 0.16 mg/kg, 22.31 mg/kg and 10.17 mg/kg, respectively. After the estimations of dietary exposure, the acceptable daily intake (ADI), the average daily dose (ADD), the provisional tolerable weekly intake (PTWI) and the relative hazard of heavy metals were evaluated. As the results, the relative hazards compared to PTWI in samples were below the recommended standard of JECFA as Pb 3.1%, Cd 0.9%, Hg 0.5%. Cancer risks through slope factor (SF) by Ministry of Environment Republic Korea and Environmental Protection Agency was 4.24 × 10−7 for Pb and 3.38 × 10−4 for As (assuming that the total arsenic content was equal to the inorganic arsenic). Based on our results, possible Pb-induced cancer risks in herbal pills according to parts used including cortex, fructus, herba, radix, seed, algae and others were 1.95 × 10−7, 1.45 × 10−6, 2.14 × 10−7, 6.27 × 10−7, 1.99 × 10−8, 3.61 × 10−7 and 9.64 × 10−8, respectively. Possible As-induced cancer risks in herbal pills by parts used including cortex, fructus, herba, radix, seed, algae and others were 1.54 × 10−5, 7.24 × 10−5, 1.23 × 10−4, 2.02 × 10−5, 3.25 × 10−6, 2.18 × 10−3 and 5.67 × 10−6 respectively. Taken together, these results indicate that the majority of samples except for some samples with relative high contents of heavy metals were safe.

This study was carried out to investigate antibiotic resistances of 181 isolates in Staphylococcus aureus isolated from 450 specimens which were a General Hospital personnel in Cheonan province. Of the 181 isolates, 89 isolates (49.2%) were methicillin resistant and 92 isolates were susceptible. They were resistant to penicillin (80.1%), clindamycin (18.8%), oxacillin (49.2%), cefepime (28.2%), cefotetan (34.3%), ampicillin (66.3%), gentamicin (27.6%), tetracycline (17.7%) and erythromycine (43.1%), also. In resistant rates of the 89 MRSA, 5 antibiotics were more than 50%, but in those of the 92 MSSA, only 1 antibiotics was. Also they showed very highly multi-drug resistance patterns to 15 antibiotics as follow : One hundred and forty five isolates(82.3%) showed antibiotic resistance to more than one kind of the 15 kinds antibiotics. In the multiple resistant patterns, one drug resistant isolates were 18 isolates (9.9%), 2 drugs 29 (16.0%), 3 drugs 21 (11.6%), 4 drugs 29 (16.0%), and more than 5 drugs were 52 isolates (28.7%). The number of more than 5 drugs resistant strains in MSSA were only 1 strain, but those in MRSA were 51 isolates (57.3%). Forty five isolates (24.9%) produced enterotoxin serotype C and all MRSA.

In order to investigate phenotype and genotype of Salmonella enterica subsp. enterica serovar Enteritidis, Forty-eight S. Enteritidis isolates from diarrhea patients were analysed using antimicrobial resistance typing, Phage typing, and Pulsed field gel electrophoresis in Seoul from 2004 to 2005. All of S. Enteritidis were resistant to streptomycin(SM, 37.5%), ampicillin(AM, 43.8%), t icarcillin(TIC, 43.8%), chloramphenicol(CM, 29.2%), t etracycline(TE, 10 .4%) and nalidixic acid(NA, 18.8%) among 16 antimicrobial drugs. Of 48 S. Enteritidis, 8 isolates(16.7%) were resistant to 1 drug, 3 isolates( 6.3%) to 2 drugs, 1 isolate (2.1%) to 3 drugs and 17 isolates(35.7%) to 4 drugs. The basic pattern of 4 drugs resistance was SM, TIC, TE, and CM but 1 drug resistant isolates represent all nalidixic acid resistance. Among 30 antibiotic r esistant S . Enteritidis, 2 1 isolates(70 %) were phage type 2 1, 8 i solates(26.7%) were phage t ype 2 3 and 1 isolate( 3.3%) was RDNC, respectively. Of the phage types observed, all of phage type 23(8 isolates) were nalidixic acid resistant and phage type 21 were AM-TIC-SM-CM multi-resistance(13 isolates; 43.3%), AM-TIC-SM-TE(4 isolates; 13.3%), AM-TIC-SM(1 isolate; 3.3%), AM-TIC-CM(1 isolate; 3.3%), and AM-TIC(2 isolates; 6.7%) resistance and 1 isolate of RDNC was NA-TE resistance. PFGE divided the isolates into two major clusters, A(n=14) and B(n=14). There were four different resistance profiles with resistance to AM, TIC, SM, TE, NA within PFGE A. Also resistance to AM, TIC, SM, CM was common within PFGE B. The PFGE A strains typed as PT21(n=5), PT23(n=8), and RDNC(n=1), While all the PFGE B strains typed as PT21(n=14). In consequence, there was the highly significant concurrence between resistance typing, phage typing and PFGE.

To investigate antimicrobial susceptibility and relationships of the multidrug resistance and phage types of 49 Salmonella enterica serovar Typhimurium isolated from diarrhea patients in Seoul between 1999 and 2002, we analyed stranis by serotyping, antimicrobial susceptibility test and phage typing. Out of 2,705 samples examined for the causative agent from diarrhea patients, total of 512 isolates were confirmed to be genus Salmonella. Out of 512 isolates, 49 isolates(9.6%) were identified as S. Typhimurium. All of the S. Typhimurium strains represented 100% susceptibility to ceftriaxone, cefoxitin, amikacin and ciprofloxacin and were over 90% susceptibility to gentamicin, cephalothin and kanamycin, but were resistant to tetracycline(75.5%), streptomycin(59.2%), sulfonamides(55.1%), ticarcillin(36.7%), ampicillin( 28.6%), and chloramphenicol(20.4%). Out of 49 S. Typhimurium, only 3 isolates(20.4%) were resistant to one drug, 6 isolates(12.2%) to two drugs and 12 isolates(24.5%) to 3 drugs, 1 isolate(2.0%) to 4 drugs, 2 isolates (4.1%) to 5 drugs, and 6 isolates(12.2%) to 6 drugs and 5 isolates (10.2%) to 7 drugs. Out of 49 S. Typhimurium, 10 isolates(20.4%) were DT195, 5 isolates(10.2%) were DT193 and DT206, 4 isolates(8.2%) were DT104L, DT146, DT203 and RDNC, respectively. Phage types observed the resistant patterns of more than 6 drugs were DT104L, DT193, DT194, DT195 and DT67, but those of 3 drugs representing S-S3-TE type was DT195.

In order to investigate the classification and antibiotic resistance of Salmonella species 718 isolates were isolated from patient in Seoul from 1996 to 2001. The two hundred and ninety eight isolates (41.5%) were identified as Sal. Enteritidis, followed by Sal. Typhi 218 isolates (30.4%), and Sal. Typhimurium 87 isolates (12.1%). The identified Salmonella species were most resistant to tetracycline (32.7%), followed by streptomycin (28.0%), ticarcillin (18.1%) and ampicillin (12.4%). Among isolates, 34.7% of Sal. Enteritidis were resistant to tetracycline, 32.3% to streptomycin, 23.2% to ticarcillin, 13.5% to ampicillin, respectively. 13.8% of Sal. Typhi were resistant to streptomycin, 10.6% to tetracycline, respectively. 66.7% of Sal. Typhimurium were resistant to tetracycline, 42.5% to streptomycin, 28.7% to ticarcillin, 26.4% to ampicillin and 17.2% to chloramphenicol, respectively. Of 718 isolates, 324 isolates (45.1%) were resistant to 1 or more drugs and 64 isolates (19.8%) were resistant to 1 drug, 132 isolates (40.7%) were resistant to 2 drugs, 50 isolates (15.4%) were resistant to 3 drugs, 27 isolates (8.3%) to 4 drugs, 27 isolates (8.3%) to 5 drugs, 22 isolates (6.8%) to 6 drugs. The most prevalent multiple resistant pattern was tetracycline-kanamycin (35.5%), followed by tetracycline-kanamycin-ticarcillin (8.3%), and tetracycline-kanamycin-ticarcillin-ampicillin (7.4%). Antibiotic resistant rate of Sal. Typhimurium was 73.6%, followed by Sal. Enteritidis 53.7% and Sal. Typhi 19.3%. Most Sal. Enteritidis was resistant to 1 drug or 2 drugs, whereas Sal. Typhi and Sal. Typhimurium were more resistant to 5 (16.7%) or 6 drugs (26.6%). The old generation antibiotics such as ampicillin, tetracycline, and streptomycin were annually more resistant than the new generation antibiotics such as ceftriaxone, ciprofloxacin or cefoxitin.

In order to investigate the microbiological contamination of spring water, we performed the standard plate count, coliform and psychrotrophilic bacteria in 109 spring waters in Seoul. Of 109 spring waters, geometric mean of standard plate count was 0.19 CFU/ml, and the highest in Mt. Boolam, 4.43 CFU/ml and Mt. Dobong, 3.86 CFU/ml, but not detected in Mt. Woomyun and Mt. Cheonggye. Four spring waters have shown over 100 CFU/ml in standard plate count. The geometric mean of psychrotrophilic bacteria was 49.2 CFU/ml, the most prevalent spring water was Mt. Nam, 125 CFU/ml, the lowest Mt. Woomyeun. Among a total of 109, coliform was detected from 21 spring waters(19.3%) and the geometric mean of colirorm was 0.005 MPN/100ml. The isolated genera of coliform were 7 isolates of E. coli(33%), 5 Klebsiella(24%), 4 Enterobacter(19%), 3 Citrobacter(14%) and 2 Serratia(10%), respectively. The 22 spring waters(20.2%) failed to meet the standard limits of drinking water based on regulation in Korea. The inappropriate rate of standard plate count in spring water was 4.5%, that of coliform was 81.1% and both of them was 13.6%. There was the significant correlation between standard plate count and psychrotrophilic bacteria in spring water(r=0.95, p<0.01).

Staphylococcus aureus is gram positive, facultatively anaerobic, non-sporulative coccus, and positive for coagulase and DNase. The food oisoning outbrealc of Staphylococcus aureus increases in the world, and third occurnn happened in our country. Of 105 isolates (25.4%) obtained 413 fecal samples of food-poisoning suspicious patients. In those cases, the enterotoxins were detected firom a total of 45 isolates (42.9%), 9 isolates(20.0%) were A type, 33 isolates (73.3%) we H types, 2 isolates (4.4%) were G type and 1 isolate was a I type enterotoxin. Among the isolates possessing staphylococcal enterotoxins, 29 isolates had H type only(64.4%), 5 isolates had A type only and 4 isolates had both A and H type. 'Iieo isolates had G type only and 1 isolate had I type only. In the antibiotic susceptibility, 48 isolates (46%) had at least one antibiotic resistance among 105 isolates, 34 isolates (70.8%) were resistant to penicillin, 1 isolate (2.1%) to ampicillin, 3 isolates (6.3%) to erythromycin and kanamycin. Seven were resistant to more than two antibiotics and especially 1 isolate was resistant to penicillin-ampicillin-nitrofurantoin.

A total of 136 coliform bacteria isolated from spring water (112 strains) and ground water (24 strains), submitted to Seoul Health and Environmental Research Institute from June to July in 1997, were characterized biochemically and microbiologically. Colonical characteristics of each isolate were also noted, including color and texture on EMB agar. Among the 136 isolates, 50.7% were greenish metallic sheen color, 44.2% were pink and 5.1% were violet. The sixty four percent were smooth, 34.6% were mucoid and 0.7%. were rough. Twenty three bacterial species were identified by IMViC and API 20E test. Among the 136 coliform bacteria known to species, 39 isolates (28.6%) were Escherichia spp., 32 isolates (23.5%) were Klebsiella ssp., 30 isolates (22.1%) were Enterobacter spp., 19 isolates (14.0%) were Serratia spp., 6 isolates (4.4%) were Citrobacter spp., 4 isolates (3.0%) were Kluyuera spp. and 7 isolates (5.1%) were other bacterial species. Strains, which were gas-positive in lactose broth but gas-negative in Kligler Iron Agar were Ent. intermedium, Ser. liquefaciens, Ser. marcescenes and Salmonella arizoae. Strains, which were H2S production were also Kleb. pneumoniae, Kleb. oxytoca, Kleb. ornithinolytica, Ent. sakazakii, Ent. cloacae, Ser. liquefaciens, Ser. ficaria, Cit. freundii and Sal. arizoae. In the present study, most of coliform isolated from spring and ground water were E. coli, Klebsiella spp. and Enterobacter spp. Since coliform with pink colony in EMB agar was isolated as frequent as coliform with greenish metallic sheen colony, coliform with pink colony should be considered as important colony. Our results suggested that new coliform strains may be emerging on the basis of biochemical and microbiological testes.

This study was carried out to isolate and identify Salmonella species from poultry slaughterhouses and pork meat processing plants during the period from January 1997 to August 1998, and analyze resistance of antimicrobial agents and plasmid profiles of isolated Salmonella strains. A total of 15 Salmonella strains was isolated from poultry carcasses, swine carcasses, pork meats and cutting boards. Identified Salmonella strains were S. typhimurium, S. heidelberg, S. hilingdon, S. mbandaka, and S. virginia. Ten (66.7%) of 15 Salmonella strains showed resistance to antimicrobial agents and five strains (33.3%) of them were resistant to two or more antimicrobial agents. Plasmids were isolated from three Salmonella isolates which had two or more plasmids.

There were few data for the distribution of the indicator organisms in the commercial plant foods, and for the normal flora and for the foodborne agents within the country. First of all it must be investigated the distribution of the indicator organisms. And also it is very important to prepare the sanitation criteria for the plant foods through the microbiological e×amination and the investigation of tendency to change of the indicator organisms according to the storage temperature and period. The average number of total viable counts for grains was 2.9 × 10^5/g, psychrophilic bacteria 2.9 × 10^6/g, heterotrophic bacteria 3.1 × 10³/g, heat-resistant bacteria 2.1 × 10³/g Pseudomonas aeruginosa 23/g. That for beans was 6.3 × 10²/g, psychrophile 34/g, heterotroph 1.7 × 10²/g. That for sesames was 1.4 × 10^5/g, coliform 350/g, psychrophile 7.4 × 10⁴/g, heterotroph 5.8 × 10⁴/g, Pseud. aeruginosa 2.3 × 10³/g. heat-resistant bacteria 150/g. That for potatoes was 2.0 × 10^7/g, coliform 5.0 × 10⁴/g, psychrophile 1.8 × 10^7/g, heterotroph 1.4 × 10^7/g, heatresistant bacteria 3.3 × 10¹/g, Staphylococcus 2.7 × 10^5/g, fecal streptococcus 4.5 × 10³/g, Pseud. aeruginosa 7.0 × 10³/g. That for mushrooms was 1.2 × 10^8/g, psychrophile 9.4 × 10^7/g, heterotroph 1.0 × 10^9/g, heat-resistant bacteria 1.6 × 10^5/g, Pseud. aeruginosa 1.3 × 10³/g. That for vegetables was 5.9 × 10^(11)/g, coliform 1.8 × 10^6/g, psychrophile 1.1 × 10²/g, heterotroph 8.4 × 10^(11)/g, heatresistant bacteria 7.6 × 10^6/g, Staphylococcus 1.1 × 10^7/g, fecal streptococcus l.1 × 10⁴/g, Pseud. aeruginosa 5.2 × 10⁴/g. That for nuts 3.9 × 10⁴/g, coliform 3.9 × 10³/g, psychrophile 4.0 × 10^8/g, heterotroph 3.2 × 10^8/g, heat-resistant bacteria 400/g. In commercial grains and beans, SPC, psychrophile, heterotroph and heat-resistant bacteria stored at 10℃, 20℃, 30℃ were constant. Staphylococcus, coliform, Pseud. aeruginosa were decreased a little in grains, but were not detected in beans. In mushrooms, all indicator organisms were increased as time goes on and were increased rapidly at 20. In sesames, coliform was not detected at all temperature. psychrophile was increased for 7 days, the otners were constant. In potatoes, SPC, psychrophile, heat-resistant bacteria, heterotroph had a tendency to increase and the others were constant. In vegetables, indicator organisms were had a tendency to increase, psychrophile, heterotroph were rapidly increased after 7 days. In nuts, SPC, coliform, psychrophile, heterotroph, heat-resistant bacteria, Pseud. aeruginosa were constant, staphylococcus and fecal streptococcus were not detected.

The average number of total viable counts for the commercial pork tested was 19/g, coliform 1.8/g, psychrophilic bacteria 15/g, heterotrophic bacteria 12/g, fecal streptococcus 6.2/100 g, Pseudomonas aeruginosa 13/100 g and none of heat-resistant bacteria and Staphylococcus was detected. That for the commercial beef tested was 130/g, coliform 5.2/g, psychrophile 140/g, heterotroph 28/g, Staphylococcus 1.2/g, fecal streptococcus 9.5/100 g, Pseud. aeruginosa 1.9/100 g and heat-resistant bacteria was not detected. That for the commercial chicken tested was 8800/g, coliform 53/g, psychrophile 4600/g, heterotroph 4700/g, fecal streptococcus 9.9/100 g, Pseud. aeruginosa 2.5/100 g. That for milk was 4700/ml, psychrophile 120/ml, heterotroph 420/ml and the others were not detected. That for the commercial cheese was 3.2/g, psychrophile 2.3/g, heterotroph 1.6/g, Staphylococcus 1/g, fecal streptococcus 9.1/g. That for fermented milk was 10^7/ml, heatresistant bacteria 10^6/ml, fecal streptococcus 2400/100 ml, lactobacillus 3.2 × 10^(15)/ml, in accordance with lactic acid bacteria and the others were not detected. There was not detected any indicator organisms from ham, sausage, butter, eggs and quails in the commercial fooods tested. SPC, coliform, psychrophile and heterotroph in commercial meats stored at 10℃ were increased rapidly as time goes on but heat-resistant bacteria, staphylococcus, fecal streptococcus and Pseud. aeruginosa were constant. At 20℃, SPC, coliform, psychrophile, heterotroph and fecal streptococcus were the highest at 7 days and heat-resistant bacteria, staphylococcus and Pseud. aeruginosa were increased a little. At 30℃, all indicators were increased rapidly for 3 and 7 days and then decreased rapidly. All indicator organisms were increased at the level of 10/g for 14 days in meat products stored at 10℃, but SPC, psychrophile and heterotroph in meat products stored at 20℃ were increased at the level of 10^5/g. It showed that the indicators in meat products stored at 30℃ had a tendency to increase at the level of l0₂/g relative to those stored at 20℃. SPC, psychrophile and heterotroph in milk stored at 10 increased up to the level of 10₄/ml, but coliform, staphylococcus, fecal streptococcus and Pseud. aeruginosa were not detected. As stored at 20℃ and 30℃, they were increased rapidly for 1 or 3 days and then constant for a long time.

In order to investigate the species, serobiological characteristics and antibiotic sensitivity of Pseudomonas spp, we isolated Pseudomonas spp from 57 spring waters around Seoul area for spring, summer and autumn and identified Pseudomonas spp by biochemical characteristics and serological method. And also we tested the antibiotic sensitivity test by discdiffusion method. Of 57 spring waters tested, Pseudomonas spp were isolated from 33 spring waters(57.9%). Isolation rate of Pseudomonas spp in spring season was 28.1%, summer 21.1% and autumn 28.1%. Only 1 spring water was detected Pseudomonas spp in all seasons and 9 (15. 8%) were detected for 2 seasons and 13 (22.8%) were for only 1 season. Isolation rate of Pseudomonas spp at Mt. Cheonggye was 50% and followed by Mt. Bookhan 35.7%, Mt. Daemo 33.3%, Mt. Dobong 29.6%, Mt. Surak 25.9%, Mt. Woomyun 22.2% and Mt. Bulam 7.4%. Of 44 Pseudomonas spp, 22 strains (50%) were identified by Ps. putida, Ps. aeruginosa, Ps. tluorescens and Ps. mendocina were identified 6 strains (13.6%), respectively. 4 strains (9.1%) were identified by Ps. aureofaciens. Of 6 Ps. aieruginosa, serotype A was 2 strains, B, E, G, and K was 1 strain, respectively. Of 44 Pseudomonas spp, resistance rate to amoxicillin was 90.9% and followed by chloramphenicol 84.1%, tetracycline 84.1%, carbenicillin 81.8%, nalidixic acid 68.2%, neomycin 38.6%, streptomycin 31.8%, gentamicin 4.6%, kanamycin 4.6% and colistin 2.3%. Ps. aseruginosa was more sensitive to carbenicillin than other Pseudomonas spp isolated from spring waters in Seoul area but more resistant to kanamycin, and Ps. aureofaciens was no resistant to streptomycin. Among multiple drug resistance, resistance to 5 drugs was 31.8%, 4 drugs 15.9%, 7 drugs 13.6%, 1 drug and 2 drugs 4.6%, and 8 drugs 2.3%, respectively. The multiple resistance patterns detected highestly were NA-CB-C-TE-AMC (18.2%), NA-CB-N-C-TE-AMC (13.6%), CB-C-TE-AMC (11.4%) and NA-CB-N-C-TE-AMC-S (9.1%).

In order to investigate bacterial sanitary condition of fish and shellfish, we examined the normal flora in the 25 species of commercial fish and shellfish, and also proportional change of normal flora by storage period and temperature. Isolated 334 were isolated in the normal fish and shellfish and predominant genera were Pseudomonas (25.2%), Staphylococcus (10%), Acinetobacter (7.2%), Vibrio (6.9%), Micrococcus (5.4%), Aeromonas (5.2%), and Enterobacter (5.2%). In accordance with storage period and temperature, Pseudomonas grew on high ratio at 10℃ steadily, but Proteus had increased proportionally at 20℃ and 30℃. Additionally, Citrobacter, Moganella, and Pasteuralla had increasd, while Achromobacter, Acinetobacter, Yeast, and Micrococcus had decreased by period.

This paper intends to investigate commercial fish and shellfish 25 species (fish 8 species, shellfish 7 species, crustacean 3 species, molusc 4 species and echinodermata) for the distribution of sanitary indicator organisms (total viable counts, coliforms, staphylococci, vibrios, and enterococci) and diatributional change of indicator organisms according to storage temperature and period. The logarithmic mean of total viable counts for total commercial fish and shellfish 25 species was 5.41±0.26 CFU/g, and in accordance with fish and shellfishes, crustacean 6.76±0.67 CFU/g, shellfish 5.67±0.56 CFU/g, echinodermata 5.47±0.50 CFU/g, fish 5.021±0.38 CFU/g, and mollusc 5.03±0.65 CFU/g. The logarithmic mean of enterococci was 2.36±0.37 CFU/g, and in accordance with fish and shellfish, crustacean 3.44±0.12 CFU/g, shellfish 3.87±0.45 CFU/g, echinodermata 3.38±0.0 CFU/g, fish 2.16±0.41 CFU/g and mollusc 0.01±0.0 CFUIg. The logarithmic mean of vibrios was 1.60±0.59 CFUIg, and in accordance with fish and shellfish, crustacean 4.23±0.11 CFU/g, shellfish 3.58±0.90 CFUIg, echinodermata 1.64±0.34 CFU/g, fish 1.79±0.67 CFU/g and mollusc 1.07±0.61 CFU/g. The logarithmic mean of staphylococci was 1.60±0.59 CFU/g, and in accordance with fish and shellfish, shellfish 0.01±0.00 CFU/g, echinodermata 3.51±0.60 CFU/g, fish 1.68±0.64 CFU/g, crustacean 0.34±0.33 and mollusc 2.90±0.11 CFU/g. The logarithmic mean of coliforma was 2.2±0.32 CFU/g, and in accordance with fish and shellfish, echinodermata 3.58±0.89 CFU/g was highest, shellfish 3.25±0.30 CFU/g, crustacean 3.23±0.49 CFU/g, fish 2.18±0.63 CFU/g, peeled shellfish 1.80±0.51 CFU/ g and mollusc 1.55±0.95 CFU/g. As the results of research of the change of the contaminated indicator microflora in working with storage period at 10℃, 20℃ and 30℃, total viable counts was increased without storage temperature and enterococci were decreased slowly at 10℃, but increased at 20 and 30℃ Vibrios were decreased slowly at 10℃, decreased at 20℃ and 30℃ in 2 days after increased rapidly. Staphylococci were increased promptly without storage temperature in 2 days, then the total viable counts were maintained. Coliforms were increased at 10℃ by 7 days, then decreased or maintained after 14 days, changed at 20℃ in accordance with fish species in 2 days, then returned to the initial total viable count, and decreased rapidly at 30℃ on 2 days. By the way, there were no difference among the species.

목장에서 가축음용수 빛 세척수로 사용하는 목장용수에 대한 위생화학척 상태률 계절척으로 조사하기 위하여 강훤도 및 충청도에 소재한 유우목장 58 곳에서 채취한 목장용수에 대하여 pH, 총경도, 과망간산칼륨소비량, 질산성 질소, 암모니아성 질소, 염소이온, 황산이온, 불소, 철, 납, 카드융, 아연, 망간, 동 및 크롬올 봄과 여름 2 회 조사하였다. 충남지역과 강원지역 목장용수의 pH 는각각 6.49:t 0.09, 6.70 :t 0.06 이었으며， 총경도 9O.2l:t 7.07, 64.53:t 6.38 mg/l, KMn04 소비량 4.13:t 0.62, 4.34 :t 0.26 mg/l , 질산성질소 6.51:t 0.55, 3.6l:t 0.58 mg/l , 염소이온 20.51 :t 1.99, 5.41 :t 1.36 mg/l 그리고 황산이온 6.61 :t 1.02, 7.28:t 1.30 m g/l이었으며, 암모니아성 질소는 거의 검출되지 않았다. 검사한 중금속 불소, 철, 납, 카드륨, 아연, 망간, 동 및 크롬은 검출되지 않았거나 극미량만 검출되었다. 검사항목간의 관계를 비교하면 총갱도, 철산성질소, 염소이용 및 황산이 옹은 검사항목간 상관관계를 나타내었다. 검사항목 중 충남과 강훤 목장용수간 지역켜 차이률 나타낸 항목은 총갱도, 질산성질소 및 염소이온이였으며, 봄과 여름에 차이를 나타낸 항목은 질산성질소 뿐이었다.