간행물

한국식품위생안전성학회지 KCI 등재 Journal of Food Hygiene and Safety

권호리스트/논문검색
이 간행물 논문 검색

권호

Vol.10 No.4 (1995년 12월) 11

1.
1995.12 구독 인증기관 무료, 개인회원 유료
The antifungal effects of polyphosphates on the growth and T-2 toxin production of Fusarium sporotrichioides M-1-1 were investigated. The growth of the strain was significantly inhibited in the potatoes dextrose agar medium treated with 1.5% polyphosphates or more. When we checked T-2 toxin by the indirect competitive ELISA, the strain produced 11.25 ug/ml and 10.90 g/ml levels of T-2 toxin in rice and corn containing 50% moisture contents, respectively. However, T-2 toxin was little detected in rice medium and corn medium with 1.5% polyphosphates addition for short(14 days) and prolonged incubation time(45 days). We also observed the destruction of cell wall and outflow of cell ingredients with 1% polyphosphates treatment to the strain. Therefore, moisture and polyphosphates greatly effected on the growth and T-2 toxin production of the strain.
4,000원
2.
1995.12 구독 인증기관 무료, 개인회원 유료
To investigate the antimicrobial effect of polyphosphates as a food additive, the growth and structural change of Listeria monocytogenes Scott A were examined in relation to polyphosphates concentration and incubation temperature. Up to 10,000 ppm of polyphosphates, the growth rate of strain was gradually inhibited with increasing polyphosphates concentration and decreasing the incubation temperature. Minimal inhibitory concentration of polyphosphates to the growth of strain was about 12,000 ppm. It was observed, using both scanning electron microscopy(SEM) and transmission electron microscopy(TEM), that 0.9% polyphosphates treatment was resulted in the destruction of cell wall and outflow of cell ingredients. The antimicrobial effects of polyphosphates were more effective than those of dehydroacetate and potassium sorbate at 13℃ and 4℃. The growth rate of the strain in beef was significantly inhibited by the treatment of 0.9% polyphosphates and storaged at cooling temperature.
4,000원
3.
1995.12 구독 인증기관 무료, 개인회원 유료
A screening method has been developed for detecting sulfamethazine(SMZ) contamination of meat or feeds by using horseradish pero×idase (HRP) labeled protein A (Prot A-HRP)and an indirect competitive enzyme-linked immunosorbent assay(ELISA). The assay is based on competitive binding of guinea pig anti-SMZ with SMZ in sample and SMZ-gelatin con$lt;jugate(SMZ.GEL) followed by the uptake of prot A-HR,P onto polystyrene microwell plate coated with SMZ.GEL. Percent binding B/Bo × 100) was calculated from the absorbance in the absence (BO) and presence (B) of SMZ. By the sandard curve prepared by plotting log(SMZ) vs percent binding of each lmown reference solution, the detection limit was 1.0 ppb or leas. Cross reaction with sulfadimethoxine, sulfaguaniding, sulfamerazine, sulfamthoaypyridazine, sulfanilamide, aulfisomidine and aulfisoxazole were not observed. But sulfamerazine crosareacted in the test. The EC-50 value (concentration causing 50% inhibition of color development compared with blank) of sulfamerazine was 2.0 ppm. Further quality control will make the ELISA system ideal for the detection of SMZ in meat or feeds.
4,000원
4.
1995.12 구독 인증기관 무료, 개인회원 유료
The antimutagenic effects of 46 kinds of medicinal plants that have been used as traditional folk antitumor agents in Korea were studied by using Ames mutagenicity teat. Most of the methanolic extracts from the plants which were used in this experiment showed strong antimutagenic activity toward atlatoxin B₁(AFB₁) in Salmonella typhimurium TA100 and TA98. However, N-methyl-N'-vitro-N-nitrosoguanidine (MNNG) induced mutagenicity was not blocked by adding the methanolic extracts of the plants except persimmon leaves (Diospyros kaki Thunberg) and Elaeagnus umbellata.
4,000원
5.
1995.12 구독 인증기관 무료, 개인회원 유료
The ethanol extract from the root bark of Morus alba showed the strongest antimicrobial activity on the growth of almost all the tested microorganisms which were food-borne pathogens and food-related microorganisms. In order to isolate and purify of antimicrobial substance extracted from the root bark of Morus albs, the antimicrobial substance from the ethanol extract which exhibited a strong antimicrobial activity was purified by solvent fractionation, silica gel column chromatography, TLC and HPLC. Among the fractions fractionated by 4 kinds of solvents from the ethanol extract, the antimicrobial activity of ethyl acetate fraction had the strongest antimicrobial activity against B. subtilis. Unknown compounds were isolated from the ethyl acetate fraction by silica gel column chromatography, TLC and HPLC and the compounds showed strong absorbance at 207, 217 and 285 nm, therefore, it was supposed to be a kinds of aromatic compound.
4,000원
6.
1995.12 구독 인증기관 무료, 개인회원 유료
Bacillus subtilis SH-1 have been isolated and identified from coastal sea, in Pusan. The optimal cultural characterization of Bacillus subtilis SH-1 for the production of bacteriolytic enzyme was determined. Bacillus subtilis SH-1 produced the bacteriolytic enzyme well in the medium consist of 1.0% glucose, 1.0% yeast extract, 1.0% NaCl, 0.02% K₂HPO₄, 0.002% MgSO₄, 7H₂O, 0.001% MnSO₄, 5H₂O, and 0.0001% FeSO₄, 7H₂O. The optimal medium pH, incubation temperature, and shaking time for the highest production of the enzyme were 8.0, 30℃ and 28 hours respectively.
4,000원
7.
1995.12 구독 인증기관 무료, 개인회원 유료
Analytical method for saccharin in foods was developed using gas chromatography/mass spectrometry(GC/MS). Methylation with diazomethane, acetylation with MBTFA, and silylation with MSTFA and MTBSTFA were compared. Methylation of saccharin produced N- methylated saccharin as the major product and O-methylated saccharin as the minor one. Silylation of saccharin with MSTFA and MTBSTFA resulted in the formation of the corresponding O-silylated products, respectively. The derivatization of saccharin was optimized with MSTFA. The ions at m/z 240, 255, and 166 were monitored to characterize saccharin.
4,000원
8.
1995.12 구독 인증기관 무료, 개인회원 유료
In order to study the effects of Aloe vera Linne treatment on the clinical chemistry in patients with liver disease, seven patients were administered orally with 800-1, 600 mg of Aloe vern Linne four times a day for six months. The high levels of serum AST, ALT, ALP, γ-GTP and total bilirubin in patients were significantly reduced by administration of Aloe vem L.. The reduced serum albumin/globulin value was increased by Aloe vera L. treatment. But other blood parameters of clinical chemistry values were not affected by Aloe very L. treatment. These data suggest that Aloe very L. can be effective in treatment of the patients with liver disease.
4,000원
9.
1995.12 구독 인증기관 무료, 개인회원 유료
In order to promote the prevention of microbial and enzymatic spoilage and to retain the freshness, sweet persimmons harvested in Gyeongsangnam-do were treated with grapefruit seed extract(GFSE)-CaCO₃ mixture and stored in the proper packaging conditions. A low concentration of GFSE showed effective growth inhibition of plant pathological bacteria and fungi, Enterobacter pyrinus and Fusarium sp., which were involved in the decay of fruits and vegetables. GFSE was stable to heat treatment; its antimicrobial activity was not changed by heat treatment upto 100℃. However, when the temperature was raised to 120℃, about 90% of total activity was retained within 30 min. GFSE was also highly stable to broad pH changes; its activity was not changed in the range of pH 2.0 to pH 12.0. The physiological function of cell membrane in the spores of Bacillus cereus and the hyphae of Fusarium sp. was destroyed by treating with GFSE. It was observed that treating sweet persimmons with GFSE mixture and storing them in strech-wrapped packages could prolong the freshness of sweet persimmons and reduce quality deterioration.
4,000원
10.
1995.12 구독 인증기관 무료, 개인회원 유료
Total 29 Monascus strains were isolated from Ang-Khak and 4 of them were selects based on the relative intensity of soluble red pigment and growth rate. The optimum growth temperature of all the selected isolates was 32.5℃ on malt extract agar(MEA) plate. The optimum growth pH was 5.0 on czapek yeast extract agar plate, while it was pH 6.2 or 6.5 on MEA plate. Isolate No. 116, especially, showed the strongest antimicrobial activity aganist Bacillus subtilis and Staphylococcus aureus but much less aganist Escherichia coli and Enterobacter aerogenes. The maximun antimicrobial activity of isolate No. 116 against St. aureus was achieved at initial pH 5.3 on rice extract broth. The acitivity was increased with increasing amount of culture broth concentrate of isolate No. 116.
4,000원
11.
1995.12 구독 인증기관 무료, 개인회원 유료
Eight natural or semisynthesized monoterpenes were examined for their effects on rat brain monoamine oxidase(MAO) using benzylamine as substrate. Thujone and 3-carene were found to have the inhibition effects on rat brain MAO activity; 38% and 95% inhibition at 10^(-3) M respectively. The kinetic study on 3-carene, the most potent inhibitor tested in this study, showed that its MAO inhibition effect was confirmed as uncompetetive type. But (+) pulegon and (-) isopulegon was found to activate MAO slightly.
3,000원