Bacillus subtilis SH-1 have been isolated and identified from coastal sea, in Pusan. The optimal cultural characterization of Bacillus subtilis SH-1 for the production of bacteriolytic enzyme was determined. Bacillus subtilis SH-1 produced the bacteriolytic enzyme well in the medium consist of 1.0% glucose, 1.0% yeast extract, 1.0% NaCl, 0.02% K₂HPO₄, 0.002% MgSO₄, 7H₂O, 0.001% MnSO₄, 5H₂O, and 0.0001% FeSO₄, 7H₂O. The optimal medium pH, incubation temperature, and shaking time for the highest production of the enzyme were 8.0, 30℃ and 28 hours respectively.

• 류병호(경성대학교 식품공학과) | Beung-Ho Ryu
• 진성현(부산광역시 보건환경연구원) | Sung-Hyun Jin