피카오프레토 등 복합물의 개별인정 시험법인 총 폴리 페놀 시험법에서 Folin-phenol 시약 선정, 발색시약인 35% 탄산나트륨의 반응시간 및 온도, 시료량 등을 비교 실험 하여 총 폴리페놀에 대한 표준화된 시험법을 확립하였다. 총 폴리페놀의 표준화된 시험법의 밸리데이션을 수행한 결과 회수율은 102.3~112.4%, 정밀도의 표준편차는 0.81~ 3.18%였으며 검량선의 직선성은 결정계수(r2)가 0.999이상 을 나타냈고 검출한계는 5 μg/mL, 정량한계는 15 μg/mL 이었다. 유통제품에 대해 표준화된 총 폴리페놀 시험법의 적용성 검토를 위해 1건의 검체를 수거하여 분석한 결과, 총 폴리페놀 표시 함량 대비 105.6%로 적합하였다.

As generic health functional food items have been expanded, this research project has been conducted to prepare a scientific and systematic standardized analytical method of relevant food item and examine the suitability of the method for health/functional foods on sale. Total polyphenol was necessary for development and verification of standardized analytical method. The method exhibited high linearity in the tannic acid calibration curve (r2 > 0.999) over concentrations of 5-50 μg/mL. The limits of detection and quantitation for tannic acid were 5 μg/mL and 15 μg/mL, respectively, while tannic acid recovery was 102.3-112.4% with standard deviations of 0.8-3.2%. To verify the accuracy of the analytical method, the labeled amounts of purchased health functional foods were monitored. The recovery for tannic acid was 105.6% of the labeled amounts. Thus, the new method was suitable for all cases.

ABSTRACT
 Materials and Methods
  표준품 및 시약
  구아바잎 추출물의 분석
  피카오프레토 등 복합물의 분석
  시험법 표준화
  비타민 C 함량 측정
  밸리데이션 및 적용성 검토
 Results and Discussion
  시험법 표준화
  밸리데이션 및 적용성
  비타민 C 보정
 Acknowledgement
 국문요약
 References

• 허수정(식품의약품안전평가원 식품위해평가부 영양기능연구팀, Nutrition and Functional Food Research Team, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation) | Soojung Hu Correspondence to
• 김지안(식품의약품안전평가원 식품위해평가부 영양기능연구팀, Nutrition and Functional Food Research Team, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation) | Ji-An Kim
• 문명희(식품의약품안전평가원 식품위해평가부 영양기능연구팀, Nutrition and Functional Food Research Team, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation) | Myung-Hee Moon
• 이성혜(식품의약품안전평가원 식품위해평가부 영양기능연구팀, Nutrition and Functional Food Research Team, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation) | Sung-Hye Lee
• 윤혜성(식품의약품안전평가원 식품위해평가부 영양기능연구팀, Nutrition and Functional Food Research Team, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation) | Hae-Seong Yoon
• 홍진환(식품의약품안전평가원 식품위해평가부 영양기능연구팀, Nutrition and Functional Food Research Team, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation) | Jin-Hwan Hong