In this study, ohmic heating was applied for pasteurization of red pepper powder, and investigated its pasteurization effect. After pasteurization, seasoned squid jeotkal was manufactured by using red pepper powder, and its quality changes were investigated. On comparing sensory evaluation in squid jeotkal by pasteurized and conventional red pepper powder during storage at 5, 15 and 25℃, quality changes in squid jeotkal by the pasteurized red pepper powder. But quality change difference between two products were decrease with increasing storage temperature. Viable cell counts in two products stored at 5℃ were increased slowly until 60 days. Viable cell counts in squid jeotkal by conventional red pepper powder were increased up to ca. 10^6 CFU/g at 15, 25℃ after 30, 15 days, respectively, but that by pasteurized were ca. 10^8 CFU/g at 25℃ after 30 days. Changes in pH, VBN and NH₂-N in two products were shown difference significantly, but were decreased by increasing storage temperature. In squid jeotkal by conventional red pepper powder, the main free amino acids were glutamic acid, leucine, glycine, aspartic acid and alanine, and these amino acids held 47.95% of the total free amino acid. But in squid jeotkal by pasteurized red pepper powder, glutamic acid, glycine, aspartic acid, leucine and lysine, and these amino acids held 57.58% of the total free amino acids.