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[논문] 제초제 저항성 형질전환 벼 계통의 농업적 특성과 분자생물학적 평가 KCI 등재

Evaluation of agronomic and molecular biological characteristics of the herbicide resistance transgenic rice

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한국육종학회지 (Korea Journal of Breeding Science)
한국육종학회 (The Korean Breeding Society)
초록

Agronomic characteristics of transgenic herbicide-resistant rice lines were evaluated under field condition. Differences in agronomic traits and rice quality were observed between transgenic plants and the corresponding untransformed controls. Transgenic Ilpumbyeo lines flowered earlier than untransformed controls, whereas transgenic Nagdongbyeo flowered at the same time as untransformed control. With regard to the yield and rice quality, most of the transgenic lines showed lower yield than control except some selected transgenic lines. Selected Nagdongbyeo transgenic line also showed good eating quality comparable to the control although most selected Ilpumbyeo transgenic lines showed slightly lower eating quality and increased white center/belly in the rice grain. In order to find the main factor leading to somaclonal variation among transgenic lines, Tos17, a rice retrotransposon activated by prolonged tissue culture period was investigated. Although the transgenic lines carried only one or two of transformed bar gene, the copy number of Tos17 increased in most transgenic lines compared with control. The activation of Tos17 was not detected in selected promising transgenic lines such as ND115-15-1-B and IP23-3-3, suggesting that the increased copy number of Tos17 may have negatively affected agronomic characteristics of transgenic rice.

저자
  • 박향미(환경생명공학과) | Hyang Mi Park
  • 김율호(작물과학원 환경생명공학과) | Yul Ho Kim
  • 서정필(IKO, 작물과학원) | Jung Pil Suh
  • 최만수(작물과학원 환경생명공학과) | Man Soo Choi
  • 김기종(농촌진흥청 연구관리과) | Kee Jong Kim
  • 신동범(작물과학원 환경생명공학과) | Dong Bum Shin
  • 박장환(작물과학원 환경생명공학과) | Chang Hwan Park
  • 이장용(작물과학원 환경생명공학과) | Jang Yong Lee