간행물

한국육종학회지 KCI 등재 Korea Journal of Breeding Science

권호리스트/논문검색
이 간행물 논문 검색

권호

Vol. 39 No. 2 (2007년 6월) 28

1.
2007.06 서비스 종료(열람 제한)
In general, since partial pollen is derived to sporophyte, anther culture efficiency is low, and practical application that is introduced in actuality breeding technology is not many. This study was carried out to improve regeneration of green plants through some culture environments contol in anther culture of naked brley. Among the factors related with plant regeneration, medium was effective in component containing L-glutamine 256 mg/L, L-proline 250 mg/L, IAA 1 mg/L and BAP 2 mg/L controlled in addition sucrose 30 g/L or maltose and sucrose mixed each 30 g/L to increase both plant regeneration and green plant regeneration rate. Also, adequate content of CuSO4 was the best at 1.25 mg/L (fifty-fold), it was tendency to decrease albino production rate. Starvation was effective at 30℃, 7 days in case of Saessalbori for plant regeneration and Dooweonchapssalbori at 30℃, 10 days with increasing green plant regeneration against albino. After plant regeneration, under acclimation by hydroponics, roots and shoots were well developed at 20℃, light control as 2,000~5,000 Lux and photoperiodic reaction by 14/10 as dark/light in the early growth stage. In acclimation method, plants acclimated in the modified Yoshida solution filled-vermiculite in GP pot is superior (100%), in which was controlled by temperature 20℃, pH 6.0 and relative humid 90% over, especially, after transplanting in pot growth of root, sheath and leaf are more active in 20℃ and 5,000 Lux control. For Vernalization, plants derived from anther culture of F1 naked barley was different from their parents with normal heading plant even about 50% in the F1 hybrids whose vernalization was strong, whereas the rest of plants derived from anther culture formed rosette, showing that normal growth were impossible.
2.
2007.06 서비스 종료(열람 제한)
Agronomic characteristics of transgenic herbicide-resistant rice lines were evaluated under field condition. Differences in agronomic traits and rice quality were observed between transgenic plants and the corresponding untransformed controls. Transgenic Ilpumbyeo lines flowered earlier than untransformed controls, whereas transgenic Nagdongbyeo flowered at the same time as untransformed control. With regard to the yield and rice quality, most of the transgenic lines showed lower yield than control except some selected transgenic lines. Selected Nagdongbyeo transgenic line also showed good eating quality comparable to the control although most selected Ilpumbyeo transgenic lines showed slightly lower eating quality and increased white center/belly in the rice grain. In order to find the main factor leading to somaclonal variation among transgenic lines, Tos17, a rice retrotransposon activated by prolonged tissue culture period was investigated. Although the transgenic lines carried only one or two of transformed bar gene, the copy number of Tos17 increased in most transgenic lines compared with control. The activation of Tos17 was not detected in selected promising transgenic lines such as ND115-15-1-B and IP23-3-3, suggesting that the increased copy number of Tos17 may have negatively affected agronomic characteristics of transgenic rice.
3.
2007.06 서비스 종료(열람 제한)
This study was carried to examine the changes in kernel-greenness under different storage conditions in green-kerneled rices, Saedongchalbyeo (Korea) and Hexi41 (China). Rough and brown-rice grains were stored just after harvest under different storage conditions; low (15℃) and room temperature storage, and light and dark storage at 21℃, 24℃, and 27℃. Kernel- greenness was measured by using colorimeter. When the grains were stored in brown rice at room temperature under light condition after harvest, kernel greenness was maintained up to 4 months and gradually faded away until 8 months. However, kernel greenness was maintained up to 10 months storage after harvest at room temperature under dark condition, and up to more than 11 months at low temperature under dark. Rough-rice grains kept the greenness up to more than 11 months regardless of the light and temperature. The higher the storage temperature, the less kernel-greenness when stored in brown rice. Light was the critical factor for degradation of kernel-greenness. For long-term storage of green-kerneled rices, rough-rice storage, or brown-rice storage under low-temperature and dark condition seemed to be desirable for the maintenance of kernel-greenness.
4.
2007.06 서비스 종료(열람 제한)
Nitrogen (N) fertilization is essential for alleviating nutrient deficiencies of the world’s population by increasing rice production, one of the most important food crops of our time. Here we established an in vivo hydroponics rice seedling culture system to investigate the physio-biochemical and molecular responses of various rice genotypes to low nitrogen application. Yoshida’s nutrient solution (YS) was used to grow rice seedlings, and at three-week-old the seedlings manifested highly stable and reproducible symptoms, such as reduced shoot growth and length. Out of 12 genetically selected or tested genotypes, almost all (11 genotypes) showed varied degrees of growth reduction response to applied nitrogen (4 and 40 ppm N for treatment and control, respectively), but SR19663-B-B-34-3-3-3-1 showed similar growth as the control though its leaf width was smaller than the control. The leaves of a 11 representative low nitrogen-responsive genotype as BG90-2 were sampled for revealing the protein profiles between low and normal (control) nitrogen application by using two-dimensional gel electrophoresis (2-DGE) followed by staining of separated proteins with silver. Fifty differentially expressed silver stained protein spots were excised from 2-D gels and 41 proteins identified using high-throughput mass spectrometry (MS) using matrix-assisted laser desorption/ionization-time of flight-MS and nano electrospray ionization liquid chromatography tandem MS. These proteins could be assigned as major (energy metabolism, photosynthesis and oxidative stress) and minor functional categories, revealing many novel low N-responsive proteins, including those having energy/photosynthesis, and defense/stress, and iron homeostasis-related functions.
6.
2007.06 서비스 종료(열람 제한)
This study was conducted to clarify the relation of the species of genus Sorbus in Korea based on multivariate analysis for the morphological characteristics and DNA polymorphism. Twenty-eight quantitative characters were assessed and analyzed by the principal component analysis and UPGMA cluster analysis. From the principal component analysis of 28 quantitative characters, three principal components (PC’s) explained the variation of inter-specific relations among the genus Sorbus. The first PC’s explained 58.95% of the variation with the Eigenvalue of 16.5, and the second and third PC’s showed the Eigenvalue of 8.3 and 3.1 and explained 88.74% and 100.0% of the variation, respectively. Especially, the first PC’s was related in order of the fruit width (FW) and length of terminal leaflet (LTL), petal length (PL), width of terminal leaflet (WTL), and diameter of winter bud (DWB). The second and third PC’s were involved in order of the No. of leaflet (NL), No. of fruit per fruiting lateral (NFL), length of upper rachis (LUR), and diameter of rachis (DR), No. of pistil (NP), respectively. Cluster analysis using them UPGMA method based on the principal components of four species of the genus Sorbus divided into two groups. Group Ⅰ comprises Sorbus commixta and S. sambucifolia var. pseudogracilis, and Group Ⅱ consists of S. amurensis and S. aucuparia. The pattern of DNA polymorphism of the 56 inter-simple sequence repeat (ISSR) markers revealed that different taxa shared different sets of bands, and DNA analysis is useful for taxonomic study on the genus Sorbus.
7.
2007.06 서비스 종료(열람 제한)
This study was done to understand the G x E interaction of rice blast reaction for Japonica high quality rice varieties and to observe blast pattern for high quality varieties. Twenty one percent of the total sum of squares (SST) in blast reaction data of high quality Japonica varieties is attributed to genotype (G) by environment (E) interaction variation. This portion of blast response is higher than 8~12% of G x E effect in blast severity data obtained from various ecotypes of rice varieties. Blast response scores obtained from high quality Japonica varieties group were more severely affected by environment condition than mixed groups with Japonica and Indica varieties. Interaction Principal Component Analysis (IPCA) scores obtained from AMMI analysis for the leaf blast response implied variation of G x E interaction. Correlation analysis suggested that IPCA1 was associated with latitude, maximum mean temperature, precipitation and mean cloud amount. IPCA2 was associated with mean relative humidity, and IPCA3 was associated with precipitation and minimum relative humidity. Pattern analysis generated nine genotype clusters according to blast reaction over 11 regions. Collectively, the A, B, C, and D groups were susceptible to rice blast, where as the E, F, G, H, and I groups were relatively resistant to rice blast through multi-location blast nursery test. Relationship between the identified genes of high quality varieties and blast scores at each test site in the level of group could be analyzed based on the results from G x E Interaction analysis.
8.
2007.06 서비스 종료(열람 제한)
We performed field trials to assess levels of changes in intrinsic properties and resistance against soft rot of the potato cultivar ‘Irish Cobbler’ upon the introduction of the Shiva-1 gene. Each five lines, transformed with Shiva-1 gene controlled by the PAL5 promoter (P) and by the CaMV 35S promoter (E) were evaluated in the field. In based on evaluation of 10 defined morphological characteristics, all the transgenic clones of both lines proved to be true to type. When five agronomic characteristics were taken into account as well, all the transgenic lines except E8 were considered to be true to type. According to the result of northern blot analysis, seven (P1, P3, P4, P6, E10, E12, and E16) transgenic clones could be distinguished clearly from corresponding untransformed clones. But in three lines (P8, E5, and E8), no hybridization signal was detected. There seems to be positive correlation between the levels of resistance to soft rot and the transgenic expression at mRNA levels in P lines. But in the case of E lines, however, there doesn’t seem to be any correlation between the levels of disease resistance and mRNA expression
9.
2007.06 서비스 종료(열람 제한)
The production of hybrid onion (Allium cepa L.) seed is economically feasible using systems of cytoplasmic-genic male sterility(CMS). The identification of cytoplasm types might be helpful to development of sterile and maintainer lines. Due to this reason, a knowledge of the cytoplasm type is essential for hybrid breeding in onion. Especially, due to the biennial generation time of onion, to distinguish of onion cytoplasm type in individual plant may be helpful to reduce the efforts in a breeding program, when new sterile lines and the corresponding maintainer lines be developed. In our study, we applied the PCR-marker (orfA-501) to identify the cytoplasmic genotypes of collected 100 accessions of bulb onion. Among accessions, S-cytoplasm was found in 57 accessions. Nineteen accessions possessed only N-cytoplasm and twenty four accessions possessed both S- and N-cytoplasm.
10.
2007.06 서비스 종료(열람 제한)
The effect of osmotic condition on β-glucuronidase (GUS) transient expression was evaluated in microspore-derived embryos of wheat. Microspore explants were treated on medium containing various mannitol concentrations prior to and post bombardment with plasmid DNA pAHC25 containing uidA gene controlled by maize ubiquitin 1 (UBI1) promoter. GUS expression in the bombarded explants was examined by histochemical and fluorometric assays. Increased GUS expression was observed with mannitol treatment when compared to untreated explants. The histochemical study showed that the number of blue (GUS) foci were the highest in the bombarded explants treated with 0.6 M mannitol medium. The fluorometric assay of bombarded explants also proved 3.5-fold increase in GUS activity with 0.6 M mannitol treatment when compared to without mannitol treatment. These results indicate that 0.6 M mannitol is beneficial for improving transformation efficiency of wheat microspore-derived embryos or embryogenic calli through biolistic transformation.
11.
2007.06 서비스 종료(열람 제한)
The genus Arachis L. is distributed widely throughout South America. This genus encompasses 80 described species, and 31 species belong to section Arachis, which is divided into diploid and tetraploid species. The objective of this study was to determine the morphological characteristics and genetic relationships among A. hypogaea L. cv. Daekwang and thirteen germplasms, using RAPD techniques. Peanut plants were cultivated in pots (1.2×0.8 m2) with clay loam in a glass-covered greenhouse and genetic relationship was identified by DNA polymorphisms using PCR. Thirteen germplasms bloomed between June 18th and June 25th, the majority of which bloomed 3∼10 days later than Daekwang (June 15th). The leaf size of A. batizocoi Krapov. & W. C. Gregory was similar to that of Daekwang, three germplasms, A. monticola Krapov. & Rig., A. paraguariensis Chodat & Hassler, and A. dardani Krapov. & W. C. Gregory, were bigger than Daekwang, but the others were smaller. Twelve germplasms evidenced peduncle longer than those of A. hypogaea L. cv. Daekwang. All of the seeds of the germplasms were smaller than those of Daekwang (76.2 g), and the weight of 100 mature seeds ranged from 6.0∼36.1 g in sections Arachis, Erectoides, Procumbentes, and Heteranthaes. The genetic relationship distance was determined among Daekwang, A. sylvestris, A. monticola, and A. batizocoi.
12.
2007.06 서비스 종료(열람 제한)
Wild peanut species have proven to be an important germplasm resource. The objective of this study was to characterize interspecific crossability among 3 cultivars and 5 wild species of the peanut. Hybrids were acquired from crosses among three cultivars and five wild diploid species, respectively. A. sylvestirs A. Chev. evidenced the highest degree of crossability among the three cultivars. In the Valencia type, the combination of A. hypogaea L. cv. IT187846 and A. sylvestirs A. Chev. formed 32.7% pegs. Pods were developed in 98.8%, and seeds were produced at a rate of 49.4%, a higher percentage than was observed with the Virginia or Spanish types. Peg formation from pollinated flowers was increased by 18.4∼62.1% via the application of GA3, as opposed to the 26.3∼34.7% control value. The combination of A. hypogaea L. cv. Daekwang and A. batizocoi Krapov. & W. C. Gregory resulted in 93.9% peg formation. Seeds set in 24.2∼26.9% from the pegs, representing a 4.2∼16.3% increase over the 10.8∼24.3% control values. As the result of GA3 treatment of flowers after pollination, the peg emergence ratio increased significantly, but seed formation increased only by a small amount. The pods of the interspecific hybrid were smaller than those of A. hypogaea L. cv. Daekwang, and a portion of the pod had immature seeds or abnormal seeds. Via PCR-RAPD method, we were able to determine that the exotic DNA had been integrated from A. batizocoi Krapov. & W. C. Gregory to A. hypogaea L. cv. Daekwang.
13.
2007.06 서비스 종료(열람 제한)
Sweetpotato tips are known to be very useful parts as a vegetable because of their high nutritive values and great biomass yield. This study was carried out to select a variety that is suitable to the tips of sweetpotatoes as vegetables. Twenty-two varieties were planted in polyethylene (PE) houses and upland field. The tips were cropped, tested and their tips' external characteristics, quantity, sweetness, toughness and taste were likewise tested. This research found that the external characteristics of the sweetpotato tips were mostly colored green or greenish purple and the leaves were of heart shape. When it comes to texture, the crops from the PE houses presented softer sense than those from the upland field, and due to the blanching, the degree of hardness was reduced by 60 percent. The varieties that presented soft texture after blanching were Yulmi, Geonmi, Hwangmi, Sinchunmi, M301, Eunmi, Juhwangmi, Singeonmi and Gogeonmi. The sweetness, which gives a great influence to the taste, was found to be 3.8 Brix°% on the average for raw tips of sweetpotatoes cropped in P.E. houses, lower than the crops from the upland field which yielded 4.6 Brix°%. By varieties, those that were commonly high in both P.E. houses and upland field crops were three kinds - Geonmi, Sincheonmi and Juhwangmi. The yield derived was on the average 150 tons/ha, which was considerably high yield, compared to the greens like the Korean leeks, which were repeatedly cropped once planted, like the tips. The investigation into the external appearances, texture, sweetness and acceptability found that the varieties that are suitable for sweetpotato tips production are Yulmi, Geonmi, Hwangmi, Yeonmi, Chinmi and Juhwangmi.
14.
2007.06 서비스 종료(열람 제한)
dlm allele controlling disease lesion mimic trait is useful in basic research aimed at better understanding disease hypersensitive response and programmed cell death in soybean. Inheritance between dlm trait and any morphological trait, position of dlm allele on classical linkage group, molecular marker linked to dlm allele were not reported. Two populations [T255 (lf2lf2DlmDlm) x T363 (Lf2Lf2dlmdlm), T363 (dlmdlmp1p1) x T43 (DlmDlmP1P1)] were made to find independent assortment or linkage between dlm locus and lf2 or between dlm locus and P1 locus. The segregation ratios of 3 : 1 were observed in the F2 population and the Chi-square values strongly suggested that the disease lesion mimic and seven-leaflet trait was controlled by a single recessive gene. Glabrousness trait was controlled by a single dominant gene. Segregation ratios of 48 Lf2_Dlm_: 30 Lf2_dlmdlm: 21 lf2f2Dlm_ : 8 lf2lf2dlmdlm based on F2 phenotype showed that dlm allele was inherited independently with the lf2 allele controlling seven-leaflet trait in soybean. However, more F2 plants will be needed to confirm this result. Also, segregation ratios of 137 P1_Dlm_: 46 P1_dlmdlm: 49 p1p1Dlm_ : 16 p1p1dlmdlm from F2 phenotype confirmed strongly that dlm allele was inherited independently with the P1 allele controlling glabrous trait in soybean. This results indicate that dlm allele will not located in soybean classical linkage group 2 (molecular linkage group K) and soybean classical linkage group 16. This observation will helpful to attempt to position the Dlm locus on the soybean molecular linkage map.
15.
2007.06 서비스 종료(열람 제한)
Cheonsu is a new japonica rice cultivar tolerant to drought. This cultivar was developed from the cross Ilmibyeo// Juanbyeo/Salshare, where the parent Ilmibyeo is a japonica cultivar with high eating quality, Juanbyeo is a japonica for direct-seeding cultivation, and Salshare is a japonica weedy rice from Korea. The preliminary and replicated yield trail on Cheonsu was carried out at Yeungnam University in 2001, 2002 and 2003, and the local adaptability test was carried out by the National Seed Management Office (NSMO), Korea, in 2004 and 2005. This cultivar was registered to NSMO, Korea, as variety Cheonsu in 2006. Cheonsu has about 122 days growth duration from transplanting to harvesting. This cultivar has stature with 81cm of culm length. The milled rice yield of Cheonsu is about 5.10 MT/ha under standard fertilizer level of the ordinary transplanting cultivation. Amylose content of this cultivar is 18.3%.
16.
2007.06 서비스 종료(열람 제한)
A new barley (Hordeum Vulgare L.) cultivar ‘Wooho’ was developed by Honam National Agricultural Research Institute (HARI) in 2005. It had good forage characteristics (smooth awn) and showed high forage production in the 5 locations of paddy field. ‘Wooho’ was developed from the cross between SB87125〔Oweol//(SB77011)/SB79124 (Bengei//Hagane/ Bunong) ///Y7213-SD607-CM67-Milyang12〕and SB83024 (1012.2-IB65/Ol//Samheung/Suwon18-Gang) made in 1994. Subsequent selections were made throuhg bulked and pedigree selection methods. SB94104-B-B-B-B-79 was selected for smooth awn and agronomic performance in 2001 and placed in preliminary yield trial. In 2003, it was designated as ‘Suwon 396’ and performed regional yield trials. The average forage yield of ‘Wooho’ were 11.0 MT/ha and 10.9 MT/ha at May 20 and drought stage, respectively. The shattering rate of ‘Wooho’ was lower than that of ‘Ol’. It showed superior forage quality to the check variety when harvest early drought stage. ‘Wooho’ would be recommended as a cultivar of barley for whole crop forage with smooth awn.
17.
2007.06 서비스 종료(열람 제한)
A ruminant-palatable barley cultivar, Soman (Hordeum vulgare L.), with early maturing, high silage quality and high yield, was developed from the cross between SB79124〔Bengei-Ginomeo-Buheung-Morsen-Hagane/Gangbori〕and SB77189 〔Samjug/Riso82//Olbori〕in 1994. A promising line “SB941067-B-B-B-45” was selected in 1998. It performed well in the preliminary and advanced yield trials for two years from 2002 to 2003. The line was designated ‘Suwon 404’ through the regional yield trials (RYT) at six sites for three years from 2004 to 2006. Its growth habit was winter type (Ⅲ) with greenish leaves, medium culm and compact density of spike setting. Its heading date and early dough stage were Apr. 23 and May 26 respectively, which were 6 days earlier than those of check cultivar ‘Olbori’. ‘Soman’ showed weaker winter hardiness, but better resistance to lodging, shattering, and BaYMV (Barley yellow mosaic virus) than those of check cultivar ‘Sunwoo’. It showed 1.7% higher TDN content on whole-crop-silage than that of check cultivar ‘Sunwoo’. Its average of forage yield in regional yield trials was 10.31 MT/ha, which was 4% higher than that of the check cultivar.
18.
2007.06 서비스 종료(열람 제한)
‘Yuyeon’, a new ruminant-palatable barley cultivar, was developed from the cross between ‘Suwon311’ and SB86648〔CMB81A-2149 / Saeolbori // Buheung / Gangbori / 5 / Bera-olbori / Horisee-Buheung // bengei / 3 / Olbori-Daechi-Hagane / 4 / T.beared〕by the Honam Agricultural Research Institute (HARI), NICS, RDA in 2006. Among the cross made in 1996, a promising line, SB961012-B-B-1, showed good characteristics in potential forage yield in the yield trial tested in Suwon in 2001. In 2004, it was designated as ‘Suwon 406’ and placed in regional yield trials at five locations around Korea for three years from 2004 to 2006, and was released as the name of ‘Yuyeon’. It belong to maturity group Ⅲ and shows erect leaf type and compact spike with types of hoods. Its heading date was Apr. 27, which was 2 days earlier than that of the check cultivar ‘Olbori’ (‘Sunwoo’), and early dough stage, which was May. 30 which was same date of that of the check cultivar. ‘Yuyeon’ showed weaker winter hardiness, but better resistance to lodging, shattering and BaYMV than those of check cultivar. It showed higher crude protein content, grade of silage quality than those of check cultivar. The average forage yield in the regional yield trial was 10.47 MT/ha, which was 1% lower than that of the check cultivar. Cattles fed with silage from ‘Yuyeon’ (hood type) showed higher feed concentrate requirement (12.9 kg/day/body) and daily weight gain (1.46 kg/day) than those of cattles fed with silage from ‘Olbori’ (rough awn). This cultivar would be suitable for the area whose daily minimum temperature was above -8℃ in January in Korean peninsula.
19.
2007.06 서비스 종료(열람 제한)
‘Zasoojeongchal’ is a new colored naked barley cultivar developed from the cross between HB83072〔Tapgolbori / HB 78100-B-17-3-2〕, a disease resistant and good quality line and ‘Dokusima mochi hadaka’, colored naked cultivar with waxy endosperm by the Honam Agricultural Research Institute (HARI), NICS, RDA in 2006. An elite line CNB7-13-37-2-5-3, was selected in 2002 and designated as ‘Iksan 81’. It showed good agronomic performance in the regional yield trials (RYT) from 2005 to 2006 and was released with the name of ‘Zasoojeongchal’ possessing purple colored waxy endosperm. The average heading and maturing dates of ‘Zasoojeongchal’ were May 2 and Jun. 7, which were two and five days later than those of the check cultivar ‘Saechalssalbori’ respectively. The new cultivar, ‘Zasoojeongchal’, had 75cm of culm length that was 4cm shorter than that of ‘Saechalssalbori’, and 5.4 cm of spike length. It showed 607 spikes per m2, 63 grains per spike, 27.2 g of 1,000-grain weight, and 771 g of test weight. ‘Zasoojeongchal’ showed weaker winter hardiness, but better resistance to powdery mildew and BaYMV (Barley yellow mosaic virus) than those of the check cultivar, ‘Saechalssalbori’. It showed higher ß-glucan content, anthocyanin content, water absorption rate and expansion rate than those of the check cultivar, ‘Saechalssalbori’. Its average yield of the pearled grain in the regional yield trial was 2.67 MT/ha in upland, and 3.49 MT/ha in paddy field, which were 26% and 6% lower than those of the check cultivar, respectively. This cultivar would be suitable for the area above the daily minimum temperature of -6℃ in January in Korean peninsula.
20.
2007.06 서비스 종료(열람 제한)
Hopyeongbyeo is an anther culture derived Japonica rice (Oriza sativa L.) cultivar from the cross between Hitomebore and Hwajinbyeo by the rice breeding team of Honam Agricultural Research Institute (HARI), NICS, RDA, in 2003. This cultivar has a about 126 days growth duration from transplanting to harvesting. The short and round grain shape of Hopyeongbyeo is translucent with non-glutinous endosperm. Amylose content of rice grain is about 17.8% and high palatability of cooked rice compared with Nampyeongbyeo. This cultivar shows resistant to bacterial blight pathogenes of K1. The milled rice yield of Hopyeongbyeo is about 5.13MT/ha at ordinary transplanting culture in the local adaptability test. Hopyeongbyeo would be adaptable for the southern plain area of Korea.
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