Phomopsis seed decay (PSD) is part of a major fungal disease complex found in most soybean production areas of the world. To establish the genetic linkage map and efficient soybean breeding program, DNA markers were surveyed for polymorphism between PSD resistant (cv. PI 417479) and susceptible (cv. Hwaeomput-kong) varieties. F2, BC1F2, BC2F2 and the corresponding F2:3 population families were used to evaluate the correlation between molecular markers and resistance to PSD. In SSR analysis, Sct_033 was the most significantly associated with PSD resistance among selected eight primers. AF1, AF2, and AF3 were also selected in AFLP analysis. And a few promising lines could be selected from two times of back cross. Especially a breeding line SS01415-8-1 was developed, which has high seed quality such a large seed size, bright hilum color, round shape, and etc. It might be considered a good breeding material for new recommend variety having resistance gene to PSD with good seed quality.
This study was carried out to screen high α-glucosidase inhibitor (AGI) activity pepper lines from EMS treated mutants of pepper. The optimal concentration of EMS for inducing mutants to pepper was 0.15M and the optimal treatment time of EMS was 2 hours. With this condition, many mutants which have high AGI activity were screened and especially leaf of two lines were showed 27 times inhibitory activity on α-glucosidase comparing with control. There is no difference between mutants and nornmal plant in growth. These results indicated that AGI activity of pepper leaf would be increased by mutation.
Blast resistance of 18 rice cultivars which recommended as a high quality rice cultivars evaluated by blast nursery test, seedling test, and field screening and sequential planting methods. Sangmibyeo, Hwayeongbyeo, and Dongjin 1 were showed high resistance reaction to leaf blast at the blast nursery continuously, while Chucheongbyeo showed highest disease severity. All of the early maturing cultivars, Odaebyeo, Junghwabyeo, and Sangmibyeo, showed the lowest compatibility against 25 blast isolates. Saechucheongbyeo, a multiline variety of Chucheongbyeo genetic background showed 45.8%, half compatibility of 82.6% in Chucheongbyeo against 25 isolates screened. Diseased leaf areas (DLA, %) of cultivars were very low level at all test sites in the field test. Three cultivars, Sangmibyeo, Ilmibyeo, and Junghwabyeo didn’t show any leaf blast lesions across the test sites. The most variable incidences of panicle blast were observed on Odaebyeo which was 42.4%, 0.4%. and 0.0% at Jaecheon, Naju, and Chuncheon, respectively. In the sequential planting method, four rice cultivars, Nampyeongbyeo, Sindongjinbyeo, Hwayeongbyeo, and Junambyeo showed less than 40% disease leaf area (DLA) from 1st planting to 7th planting, so those cultivars were considered as a durable resistant rice cultivar against rice blast diseases.
This study was conducted to investigate nitrite scavenging and Angiotensin Converting Enzyme (ACE) effects by antioxidant ingredient and the contents of phenolic compounds is isolated and purified from various sweetpotato cultivars. Various sweetpotato cultivars contained 4 phenol compounds including gallic acid, chlorogenic acid, gentisic acid and caffeic acid. The range of total phenol compound contents within cvs. Saengmi, Seonmi, Hongmi and Zami was at 23.0~48.8mg/100g and cv. Zami containing anthocyanins pigment whose total phenol compound content was at 48.8mg/100g was by far higher than the other cultivars. The assay of antioxidant activity using solvent fractionated extraction methods was investigated in various sweetpotato cultivars. Antioxidant activity of ethyl acetate (41.2~86.2%) was much higher than chloroform and butanol. Antioxidant activity from cvs. Sangmi, Seonmi, Hongmi, Shinyulmi, Zami and Sinchunmi was much higher than α-tocopherol well-known as natural antioxidant. Rancidity inhibitory effect about soybean oil of extracts fractionated by ethyl acetate from cvs. Saengmi, Seonmi, Hongmi and Zami was high considerably. Ethyl acetate layer among various solvent fractionated extraction had a good effect on nitrite scavenging rate but not had a effect on AEC inhibitory. Solvent of column chloromatography to isolate antioxidant contents from ethyl acetate layer was even better on 30% (MeOH) : 70% (Water) condition. There are 5 ingredients at HPLC chromatogram and antioxidant activity effect of individual ingredients was slightly better on Rt 11.9 and Rt 16.9 fraction.
Transient expression profiles for several chimeric β-glucuronidase (GUS) gene constructs were determined in microspore-derived embryos of wheat following microprojectile bombardment. The constructs analyzed consisted of the uidA (GUS) reporter gene driven by six different promoters [Cauliflower Mosaic Virus 35S (CaMV35S), Nopaline synthase (NOS), Mannopine synthase (MAS), Chlorella Mosaic Virus Adenin methyltransferase (AMT), maize Ubiquitin 1 (UBI1), and enhanced 35S (E35S)]. The total numbers of GUS blue spot were determined manually under a dissecting microscope after histochemical staining for GUS. Results suggest that the E35S promoter is the most active and UBI1 promoter is the second active in embryos or embryogenic calli derived from wheat microspore. In addition, by flurometric assay on GUS, the E35S promoter was the best. Therefore, both UBI1 and E35S promoter are suitable for constitutive expression of the gene of interest in microspore-derived embryos of wheat. This information describing promoter functionality in wheat will be important when designing gene constructs for traits modification and when choosing appropriate cultivars for improvement through gene transfer experiments.
The genetic diversity was evaluated using RAPD and ISSR among natural populations and Korean wheat cultivars (Triticum aestivum). Understanding the genetic diversity of putative parental and wild stocks would be useful in wheat breeding programs. Ninety three populations were evaluated with fifty RAPD and three ISSR primers. A total of 185 RAPD and ISSR polymorphism were produced. These markers were considered to estimate the genetic distance among accessions. The genetic similarity ranged from 0.41 to 0.86. The dendrogram were constructed by using the UPGMA clustering algorithm based on genetic similarity. The genetic diversity within and among accession was assessed through Principal Component Analysis (PCA) for statistics analysis. In cluster analysis, four groups were clustered and 17 accessions were not clustered. The PCA was corresponded well to the result. This study provides basic information about the genetic relationships for breeding purposes.
Perilla is an oil and vegetable crop growing in Korea, Japan, and China. We have conducted a field expedition for collecting Perilla germplasm in Korea in 1998 and 2002. P. frutescens var. frutescens was found to be grown and cultivated in large scale throughout the country, whereas var. crispa was not seen. Weedy types of both var. frutescens and var. crispa were often found in roadsides, waste lands and areas around the farmer’s fields. Total number of collection was 176 with 123 accessions of cultivated type of var. frutescens, 33 accessions of weedy type of var. frutescens, and 20 accessions of weedy type of var. crispa. The accessions of cultivated var. frutescens had four different seed coat colors; white, gray, brown and dark brown. Brown and dark brown types were more frequently found than the white type and distributed from north to south of Korea, whereas the white type was mainly found in southern part of Korea. The seeds of cultivated var. frutescens were either soft or hard and large in size (> 2mm), whereas seeds of both weedy types were small and hard with dark brown color. The accessions collected will be useful for preserving genetic diversity of this crop for further breeding programs of Perilla crops.
This study was conducted to identify QTLs for some agronomic traits under two different N-fertilizer conditions. A recombinant inbred population (DT-RILs) between Dasanbyeo (a tongil type rice, derived from an indica × japonica cross and similar to indica in its genetic make-up) and TR22183 (a Chinese japonica variety), consisting of 166 F8 lines, was developed and used for mapping. A frame map of 1,409 cM containing 113 SSR and 103 STS markers with an average interval of 6.5 cM between adjacent marker loci was constructed using the DT-RILs. The DT-RILs were cultivated in ordinary N-fertilizer (N-P2O5-K2O=100-80-80 kg/ha) condition and low N-fertilizer (N-P2O5-K2O=50-80-80 kg/ha) condition in order to compare the different N-fertilizer effects. Grain yield was positively correlated with spikelet number in both conditions. Path analysis revealed that spikelet number is the most positive contributor to grain yield. Twenty-nine single QTLs (S-QTLs) and sixty-five pairs of epistatic loci (E-QTLs) were identified for days to heading, culm length, panicle length, panicle number, spikelet number, spikelet fertility, 1000-grains weight and grain yield in both N-fertilizer conditions. The phenotypic variance explained by these S-QTLs and E-QTLs ranged from 8.8% to 61.1% and from 10.0% to 55.6%, respectively. The total phenotypic variance explained by all the QTLs for each trait ranged from 15.4% to 92.3%.
“Hwanggeumchal”, a new covered waxy cultivar derived from the cross between “Chalssalbori” and “Milyang 65” with waxy endosperm and early maturing cultivars, respectively was developed at Honam Agricultural Research Institute (HARI), NICS, RDA, 2006. The origin of “Hwanggeumchal” is “Suwon 403” (SB942090-B-B-B-42-1). The initial cross was made in 1994 and the selected line showed a high yield and good quality characteristics under yield trial test in 2003. “Suwon 403” consistently performed well for three years (2004-2006) from the four locations of regional yield trial (RYT) in Korea and released as “Hwanggeumchal”. The characteristics of “Hwanggeumchal” were the following: rate I growth habit, green leaf and stem, compact spike and with long rough awns. The heading date was April 30 in upland and April 28 in paddy field, which was 1 and 4 days later than that with check cultivar, “Seodunchalbori”. The culm length was 81 cm which was 3 cm shorter than those with check cultivar. It showed spike length of 4.5 cm and 633 spikes per m2, 64 grains per spike, 27.6 g of 1,000-grain weight and 632 g of test weight. It showed stronger winter hardiness and higher resistance to barley yellow mosaic virus (BaYMV) than those with check cultivar. It had yellow aleurone and showed higher water absorption, expansion rate and total phenol content than those of check cultivar, but lower protein, β-glucan content and pearling rate. The average yield of the pearled grain in the RYT was 4.04 ton ha-1 in upland and 4.12 ton ha-1 in paddy field, which was 10% higher and 4% lower than that of the check cultivar, respectively. This cultivar would be suitable for the area above the daily minimum mean temperature of -8℃ in January in Korean peninsula.
Hovenia dulcis var. koreana Nakai is recently known to be a useful tree because of their medicinal function. In 1996, candidate trees were selected from 10 regions in Korea, and a clone bank was established in 1998 by grafted 70 candidate clones of candidate trees in order to breed high-yield superior trees. From 2003 to 2005, five clones were selected and screened by analyzing flowering and fruiting characteristics of 47 clones in the clone bank. “Poong-Sung 1”, a new high-yield superior H. dulcis var. koreana Nakai cultivar was finally selected from the 5 clones. The final selection focused on fruiting characteristics such as the number of fructify lateral (NFL), the number of average bunch per one fructify lateral (NABFL), the number of average bunch per fruiting lateral (NABPFL), the weight of fruit petiole per individual (WFPI) and the yield of individual (YI). Fruit petiole of “Poong-Sung 1” has dark brown skin color and fruit flesh color. Fruiting characteristics of “Poong-Sung 1” showed large better results and selection effects with an average of 19.3Ea (NFL), 4.75Ea (NABFL), 91.68Ea (NABPFL), 4.7 kg (WFPI) and 11.26 kg (YI) which are 158.8%, 147.1%, 233.9%, 401.7% and 412.5% superior compared to the mean of 47 clones, respectively.
A new standard rose cultivar ‘Pink Stone’ was bred from the cross between red standard cultivar ‘Red Queen’ and pink standard cultivar ‘Vivaldi’ at the National Horticulture Research Institute. The cross was made in 2001 and ‘Pink Stone’ was finally selected in 2006 after investigating characteristics for three years from 2003 to 2006. ‘Pink Stone’, a deep pink standard cultivar grows vigorously and has powder mildew resistant. The major characteristics of this cultivar are 115.1 stems/m2/year in yield, 77.7 cm in length of cut flower, 10.5 cm in flower diameter, 67.0 in petal number, and 12.0 days in vase life. This cultivar can be propagated by both cutting and grafting. The consumer’s preference of this cultivar is relatively higher than that of control cultivar, ‘Noblesse’.
‘Seohong’ was bred within the potato breeding program of National Institute of Highland Agriculture (NIHA). It was originated from a cross made in 1997 between ‘Jashim’ as a female and 93K65-1 as a male parent. Seohong was evaluated as a seedling number 97S05-1 for tuber characteristics, growth and yielding ability from 2001 to 2002, and than this clone was renamed as Daegwan 1-82. This clone was evaluated in the regional yield trial and the disease resistance in the five locations for three years. Finally, this clone was named as ‘Seohong’ and registered a new recommended potato cultivar in 2006. This cultivar has a round, shallow eye depth, light red-skinned tuber characteristics and low to medium dry matter content. It showed high resistance to common scab (Streptomyces scabies) and potato leaf roll virus (PLRV), however it was susceptible to potato virus Y (PVY) and late blight (Phytophthora infestans). Its mean tuber yields from the regional yield trial and winter cropping trial were 12 and 49 percent higher than that of ‘Superior’, respectively.
A new bright scarlet gladiolus “Adlib Scarlet” was released by the National Horticultural Research Institute (NHRI) in 2003. A cross was made between early flowering salmon line “94-18 (Arianne×Hongkwang)” and early flowering salmon cultivar “Adlib Salmon”, which was released at the NHRI in 2000. It was crossed in 1999 and finally selected in 2003 after investigation of the characteristics for 4 years from 2000 to 2003. “Adlib Scarlet” has a multiple middle sized bright scarlet florets with good simultaneous flowering. This cultivar is early flowering and moderately resistant against virus and neck rot. It has vigorous growth without stem bending. The width of “Adlib Scarlet” flower is 10.8 cm. The plant height is 132 cm. Days to flowering of “Adlib Scarlet” is average 92days in summer season.
A new bright pink gladiolus “May Queen” was released by the National Horticultural Research Institute (NHRI) in 2004. A cross was made between early flowering pink cultivar “Calista” and early flowering white cultivar “White Friendship”. It was crossed in 1999 and finally selected in 2004 after investigation of the characteristics for 5 years from 2000 to 2004. “May Queen” has a bright pink color with white core as a cut flower. This cultivar is early flowering without stem bending. It has good production of cormels and resistant to Fusarium and Thrips. The width of “May Queen” flower is 11.0 cm. The plant height is 121 cm. Days to flowering of “May Queen” is average 92days in summer season.
A new bright white gladiolus “White Lace” was released by the National Horticultural Research Institute (NHRI) in 2004. A cross was made between late flowering white cultivar “White Goddess” and vigorous white cultivar “Amsterdam”. It was crossed in 1999 and finally selected in 2004 after investigation of the characteristics for 5 years from 2000 to 2004. “White Lace” has a bright white color and multi-flowering florets more than 20 florets with good simultaneous flowering more than 8 florets. This cultivar is middle flowering without stem bending. It has good production of cormlets, vigorous growth and resistant to virus, Fusarium and Thrips. The width of “White Lace” flower is 11.5 cm. The plant height is 137cm. Days to flowering of “White Lace” is average 93days in summer season.
A new white multi-flowering gladiolus with pale greenish core “Wind Ensemble” was released by the National Horticultural Research Institute (NHRI) in 2003. A cross was made between white multi-flowering cultivar “White Nova”, which was released at the NHRI in 2002 and white line “95-24 (True Love × Madame Valdiek)”. It was crossed in 1999 and finally selected in 2003 after investigation of the characteristics for 4 years from 2000 to 2003. “Wind Ensemble” has multiple middle sized florets. This cultivar is middle flowering without stem bending. It has good production of cormlets, vigorous growth and resistant to virus, Fusarium and thrips. The width of “Wind Ensemble” flower is 11.2 cm. The plant height is 130 cm. Days to flowering of “Wind Ensemble” is average 100days in summer season.
A new Saxifraga cultivar “Snowmaple” was bred by crossing between “Jeokhoamugab” with red purple (RP68B) petals and “Hongsaja” with red purple (RPN66A) petals. The selection and flower characterization were conducted from 2004 to 2006 in greenhouse of the National Institute of Highland Agriculture. The flowering of “Snowmaple” starts on October 9 for 16 days, The number of flowers per plant was 315 with strong concurrent flowering habit. The flower shows red purple (RP62C) at opening, but It turns gradually changable from red purple to white. The flower has 5 petals, and the size is 2.0 cm in width and 2.3 cm in length. “Snowmaple” can be used for pot flower. Fifty percent shading is a good cultural practice against direct sunlight and soft rot disease would occurs usually by over-irrigation.
“Greenstar”, a new Saxifraga cultivar was bred by crossing between “Geumgongjak” with green (YG145C) petals and “Rokseon” with red purple (RP62A) petals in 2003. The selection and flower characterization were conducted from 2003 to 2006 in greenhouses of the National Institute of Highland Agriculture. The flowering of “Greenstar” starts on September 17 for 36 days and the number of flowers per plant was about 290. The flower color is a green (G142C) and the number of petals was 5. Flower width is 2.1 cm and flower length is 1.9 cm. “Greenstar” can be used for pot flower. Fifty percent shading is a good cultural practice against direct sunlight and soft rot disease would occur usually by over-irrigation.
“Miso”, A new Asiatic lily cultivar was derived from a cross between “Kinks” and a line “A61” made in 1997. Both “Kinks” and “A61” are yellow flower cultivars, while “A61” is a hybrid line. The cultivar was selected mainly for cut flower. The selection and the survey for the flower characteristics were conducted from 1998 to 2005 in vinyl tunnels and greenhouses of National Institute of Highland Agriculture. The flowering date of “Miso” is July 26. The flower has no fragrance and its medium sized flower is 13.7 cm in width. The yellow (Y9A) petal have no spot. The bulb weight of “Miso” is about 42 g and its bulb size is about 15 cm in circumference.