Lectin protein and Kunitz trypsin inhibitor (KTI) protein of mature soybean seed are a main antinutritional factor in soybean seed. The Le gene controls a lectin protein and Ti gene controls the KTI protein in soybean. Ti locus has been located on linkage group 9 in the classical linkage map of soybean. Position of Le locus on linkage map was not identified. Genetic relationship between Ti locus and Le locus could be useful in soybean breeding program for the genetic elimination of these factors. The objective of this study was to determine the independent inheritance or linkage between Ti locus and Le locus in soybean seed. Two F2 populations were developed from three parents (Gaechuck#1, T102, and PI548415). The F1 seeds from Gaechuck#1 (titiLeLe) x T102 (TiTilele) and Gaechuck#1 (titiLeLe) x PI548415 (TiTilele) were obtained. The lectin and KTI protein were analysed from F2 seeds harvested from the F1 plants to find independent assortment or linkage between Ti locus and Le locus. The segregation ratios of 3 : 1 for Le locus (129 Le_ : 44 lele) and Ti locus (132 Ti_ : 41 titi) and were observed. The segregation ratios of 9 : 3 : 3 : 1 (95 Le_Li_ : 34 Le_titi: 37 leleTi_ : 7 leletiti) between Le gene and Ti gene in F2 seeds were observed. This data showed that Ti gene was inherited independently with the Le gene in soybean. These results will be helpful in breeding program for selecting the line with lacking both KTI and lectin protein in soybean.
The germplasm of 246 rice cultivars was analysed for iron and zinc contents using a Inductively Coupled Argon Plasma (ICP) at International Rice Research Institute (IRRI) Philippines. Iron contents ranged from 2.0 to 12.0, and zinc ranged from 10.0 to 33.0 (mg/kg), showing with the mean values of 4.3 and 22.8 (mg/kg), respectively. In genotypes tested, there was approximately a two-fold difference in iron and zinc concentrations, suggesting a genetic potential to increase these micronutrients in rice grain. A highly significant positive correlation (r2=0.503) was found between iron and zinc contents. Iron contents decreased drastically as polishing time increased, whereas zinc decreased only slightly. In the interaction between genotype and environment on iron contents, genotype (G), environment (E), and the G × E interactions accounted for 69%, 5% and 26% of the sums of squares, respectively. Indicating that genotype is would be the most significant factor for the to improve iron contents of rice in rice breeding, suggesting that therefore identifying genotypes with relatively stable performance across various environments is important as staple food crops.
International conferences to block the spread of Avian bird flu occurred in Beijing, 2006 and others warned of the seriousness of the H5N1 strain. The meetings succeeded in generating billions of dollars from USA, EU and World Bank. Migratory birds seem to play a major role in the spread of the aggressive strain globally from Asia to Europe and Africa. Experiences of tolerance breeding of maize (Zea mays L.) for four decades against 20 biotic stresses suggest that the prime cause of the occurrence of H5N1 strain was due to the human beings’counter‐efforts against nature. Excessive use of chemicals (spray and injection) in the commercial poultry farms had created high selection pressure on virus. The new strain had mutated for survival. Attempting to eliminate the virus by chemicals for 100% control is a dangerous way to control biotic stresses. This can create more aggressive strains. A solution would be to build up tolerability of the commercial animals against the virus. Improvement of poultry cage environments and respect for nature must be integrated. Potential foes must be watched.
This study was carried out to investigate the chemical composition of peanut mutants induced by gamma radiation (Co-60) at 300 ㏉ in seeds of the cultivar Shinnamkwang. The protein contents of twenty-eight peanut mutants ranged from 23.3% to 31.7% and were increased by from 0.5% to 8.4% in fifteen mutants lines from the 26.8% of the original variety, cv. Shinnamkwang. Lipid contents of in mutants ranged from 43.2% to 53.5%, an increase of 0.2% to 5.7% from the 47.8% of the original variety. The range of unsaturated oleic acid in 28 mutants was from 38.9% to 56.9% an increase of from 1.3% to 14.0% from the 50.6% in the original variety. Linoleic acid, the highest unsaturated fatty acid, constituted 32.6% meanly of mutants, it was 17.4% lesser than oleic acid, ranging from 25.9% to 42.0%. Palmitic acid (16:0) contents ranged from 8.6% to 11.1%, and the mutant line-9 had the highest content. The ratio of oleic to linoleic acid was 0.9 to 2.2. A negative coefficient (r=-0.98**) was obtained between oleic and linoleic acid, but for other fatty acids, no significant relation was observed. Similarly, a negative coefficient of r=-0.68**was observed between saturated and unsaturated acids. The sucrose compositions of mutants ranged from 2.6% to 6.2%.
A total of 41 microsatellite markers were used with 29 genotypes to examine the relationship between SSR polymorphisms and N‐use efficiency related traits with a goal to identify the putative QTLs related to these traits. These primers yielded a total of 183 alleles (average 4.46 alleles per primer), and polymorphism information content (PIC) values of the SSRs ranged from 0.119 to 0.805 with mean value of 0.425. Correlation coefficients were obtained among the four N‐use efficiency traits in the 34 accessions and significant positive correlations of relative ratios between grain yield and harvest index (r=0.3404) and total dry matter (r=0.7976), while N uptake showed a moderate level of correlation with the ratios of the grain yield and total dry matter, respectively. 36.5% (15/41) SSR markers were monomorphic among the 25 japonica accessions out of the 29 accessions. Association between SSR genotypes and phenotypic performances from the total (29) or japonica (25) accessions was tested based on a single point analysis. Three putative QTL regions were detected for the ratio of grain yield. These include the chromosomal region containing the RM283 locus on chromosome 1 and RM25 on chromosome 8 (all and japonica accessions) and the region with the SSR marker, RM206 on chromosome 11 (the japonica accessions). For the total dry matter ratio, two chromosomal regions were identified as the putative QTL region. One is the region with the SSR marker, RM162 on chromosome 6 (all and japonica accessions) and the other was the one with the SSR marker RM25 on chromosome 8 (the japonica accessions). Among these markers, RM25 showed associations with both traits.
Herbicide resistance is the most common trait being tested and thus herbicide‐resistant genetically modified plants are now the most widely cultivated worldwide. Here we developed herbicide‐resistant transgenic Agrostis mongolica Roshev. by employing an efficient Agrobacterium‐mediated transformation procedure with 25.2% of transformation efficiency. The identification and employment of regenerable and reproducible type of callus was one of the most critical factors to ensure success in this study. PCR analysis confirmed that the bar transgene was integrated into the genome of transgenic plants. The expression of 35S‐bar gene was confirmed by Northern blot analysis. The transgenic plants showed complete resistance to herbicide, indicating that the bar gene is functional in transgenic plants.
We conducted this study to compare physiological response according to growing conditions between superior and inferior family of Pinus densiflora. In 1981, open-pollinated (OP) progenies of P. densiflora were planted in Chuncheon (CC) and Naju (NJ). We selected KW2 as a superior family and KW23 as a inferior family based on volume index among 30 OP progenies. We calculated general combining ability of each 30 OP progenies in each sites, and compared family growth rank. We collected needles of selected families in CC and NJ, and analyzed chlorophyll content, nitrated reductase (NR) activity, nitrogen content, and superoxide dismutase (SOD) acitivity. In CC, chlorophyll content and nitrogen content were more in needles of KW2 than those of KW23. In NJ, KW2 showed higher NR activity than KW23, and according to site, families in CC showed higher NR activity than those in NJ. SOD activities of both families were higher in NJ, and KW23 showed higher activity than KW2 in both sites. Consequently, inconsistency of the growth performance of two families was caused by different genetic and physiological responses.
This study was conducted to obtain basic information on mainstem, branch and leaf characteristics related to canopy for development of high yielding cultivar using 70 Korean soybean cultivars developed from 1913 to 2000. Variations of canopy width, branch length, and canopy width/length ratio were higher compared to other characteristics among 12 mainstem and branch characters. Variations of petiole angle, leaflet width/length ratio and compound leaf dry weight were higher than other characteristics among eight leaf characters related to plant canopy. Three classifications of soybean cultivars were used based on usage: I)soy sauce and tofu, II)bean sprout, and III)cooking with rice. Canopy width/length ratio was higher in group III, cooking with rice than group I, soy sauce and tofu, and group II, bean sprout, and there was no difference between the two, group I and group II. The total branch length/main stem height ratio was higher in group II, bean sprout and group III, cooking with rice than group I, soy sauce and tofu. Mainstem and branch characteristics related to plant canopy were classified into four groups by ratio of canopy width/length and total branch length/main stem length, respectively. Soybean cultivars with narrow canopy and high dependence of mainstem were Danweonkong, Keumkangkong, Shelby, and Shinpaldalkong. Soybean cultivars with broad canopy and high dependence of mainstem were Kanglim, Keumkangdaelip, and Jinyulkong, and a cultivar with broad canopy and high dependence of branch were Geomjeongkong 2. Leaflet length/width ratio was lowest in cooking with rice and there was no difference between soy sauce and tofu and bean sprout. Compound leaf area was largest in cooking with rice and smallest in bean sprout. Leaf petiole length was short in bean sprout and there was no difference between soy sauce and tofu and bean sprout. Leaf petiole angle was highest in cooking with rice and lowest in bean sprout. Leaf type was classified into four groups based on leaflet width/length ratio and compound leaf area, respectivly. Buseok and Taekwangkong had an oval leaflet and largest area of compound leaf. Eunhakong and Sohokong had extreme narrow leaflet and smallest area of compound leaf. Leaf petiole type was classified into three and four groups based on leaf petiole length and angle, respectively. A soybean cultivar with the shortest petiole length and smallest petiole angle was Eunhakong and cultivars with short petiole length and large petiole angle were Alchankong, Muhankong, and Pureunkong. A soybean cultivar with long petiole length and small petiole angle was Sinpaldalkong 2. Among a total of 70 Korean soybean cultivars, Eunhakong had an extreme narrow type in leaf, smallest compound leaf area, shortest petiole length, and smallest petiole angle of compound leaf.
“aegjoong” a white winter wheat (Triticum aestivum L.) cultivar was developed by the National Institute of Crop Science, RDA. It was derived from the cross “eumkang”“lgeuru”during 1996. “aegjoong”was evaluated as “ksan307”in Advanced Yield Trial Test in 2004. It was tested in the regional yield trial test between 2005 and 2007. “aegjoong”is an awned, semi-dwarf and soft white winter wheat, similar to “eumkang”(check cultivar). The heading and maturing date of “aegjoong” were similar to “eumkang” Culm and spike length of “aegjoong”were 77 cm and 7.5 cm, similar to “eumkang” “aegjoong”had lower test weight (802 g) and lower 1,000-grain weight (39.8 g) than “eumkang”(811 g and 44.0 g, respectively). It had resistance to winter hardiness, wet-soil tolerance and lodging tolerance. “aegjoong”showed moderate to pre-harvest sprouting (23.9%) although “eumkang”is susceptible to pre-harvest sprouting (38.9%). “aegjoong”had similar flour yield (72.4%) and ash content (0.41%) to “eumkang”(72.0% and 0.41%, respectively) and similar flour color to “eumkang” It showed lower protein content (8.8%) and SDS-sedimentation volume (35.3 ml) and shorter mixograph mixing time (3.8 min) than “eumkang”(11.0%, 59.7 ml and 4.5 min, respectively). Amylose content and pasting properties of “aegjoong”were similar to “eumkang” “aegjoong”had softer and more elastic texture of cooked noodles than “eumkang” Average yield of “aegjoong”in the regional adaptation yield trial was 5.88 MT ha-1 in upland and 5.35 MT ha-1 in paddy field, which was 13% and 17% higher than those of “eumkang”(5.21 MT ha-1 and 4.58 MT ha-1, respectively). “aegjoong” would be suitable for the area above the daily minimum temperature of -10℃ in January in Korean peninsula.
A New spary chrysanthemum (Dendranthema grandiflorum) cultivar ‘ater Fog’was bred by open pollination of ‘ngaesoguk’ Its characteristics were investigated three times from 2004 to 2006 under forcing culture in spring and retarding culture in autumn. The natural flowering time of ‘ater Fog’was 24 October, but year-round production was possible by shading and lighting. It had flower diameter of 2.3 cm, 46.5 petals and 34.4 flowers per stem in autumn. The petal was narrow(0.4cm) and the color of petal was white with presence of green at flower center. Days to flower from short-day start for ‘ater Fog’ was about 48 in spring. ‘ater Fog’showed the vase life of 22.6 days in autumn. This cultivar was registered for a commercialization in 2007. ‘ater Fog’would be cultured under greenhouse and rain shelter condition in Korea.
A rose cultivar “aedoji”developed at the Jeollanamdo Agricultural Research and Extention Services (JARES) is a standard type with deep Red flower. From 2000, two cultivars ‘ital’and 'Cardinal' were examined for horticultural characteristics and were included in cross-combination in 2003. Cultivar ‘ital’ a vigorous standard type, first preference with red flower, was used as a mother plant and cultivar ‘ardinal’ a red flower standard type with light color, was used as a pollen-donor plant. seventeen six breeding lines were selected in 2004 based on flower shape, flower color and abundance of petal numbers on the first selection. two breeding lines were selected in 2004 based on powderly mildew and vigor on the second selection. For three years, from 2005 till 2007, one breeding lines were selected and examined for their productivity, and then cultivar “aedoji”was developed because of distinctive characters such as light red color flower, upright stem, long vase-life and Resistance to the Powderly Mildew. A standard type with intermediate sized flower, cultivar “aedoji”shows in average 9.2 cm flower diameter, 62.0 cm flower stem length, and 9.5 days vase-life. The average productivity for three years was 167.3 stems ㆍm-2 per year and increased 2% compare to cultivar ‘iamo’with 163.9 stemsㆍm-2 per year.
A new peanut variety “harmpyeong”(Arachis hypogaea ssp. hypogaea L.) was developed at the Yeongnam Agricultural Research Institute, NICS, in Milyang in 2007. It was developed from the cross between the high-yielding cultivar “hindaekwang”and the very short stem cultivar “CGV94216” This Virginia plant typed “harmpyeong”has 33 cm stem height and 18 branch number Each pod with ellipse-shaped large kernel has two grains with brown testa and 100 seed weight was 88 g in the regional yield trials (RYT). This variety also showed more resistant to web blotch compared with check one. Especially it has resistance to lodging until harvesting owing to short stem. In the regional yield trials “harmpyeong”was outyielded than check variety by 13% with 4.35 MT/ha for grain.
A new peanut variety “ungsan”(Arachis hypogaea ssp. hypogaea L.) was developed at the Yeongnam Agricultural Research Institute, NICS, in Milyang in 2007. It was derived from a cross between a short stem cultivar “atonoka”and a high-yielding local cultivar “echeon” “ungsan”is the Virginia plant type. It has 18 branches per plant and 40 cm of main stem height. Each pod with long-ellipse shaped large kernel has two grains with light-brown testa and 100 seed weight was 95g in the regional yield trials (RYT). “ungsan”showed more resistant to web blotch compared with check variety. In the regional yield trials “ungsan”was outyielded in grain yield to the check variety by 16% with 4.45 MT/ha for grain.
A new peanut variety “eonan”(Arachis hypogaea ssp. hypogaea L.) was developed at the Yeongnam Agricultural Research Institute, NICS, in Milyang in 2007. It was developed from the cross between the erect short stem cultivar “P9128” and the high-yielding cultivar “aekwang” “eanan”which is Virginia plant type has 18 branch number per plant with early maturing and long-ellipse shaped large kernel. Each pod has two grains with brown testa and 100 seed weight was 87 g in the regional yield trials (RYT). Especially this variety has resistance to lodging owing to short stem. The free sugar and tannin content of fresh peanut are 3.7% and 0.48%, respectively, similar to check variety. In the regional yield trials “eonan”was outyielded than check variety by 14% with 8.59 MT/ha for fresh pod and by 7% with 3.75 MT/ha for grain.
A new early maturing satsuma mandarin (Citrus unshiu Marc.) ‘'Haryejosaeng’' was developed as a nucellar seedling selection of ‘'Tachima Wase’' (C. unshiu Marc.) that was crossed with C. natsudaidai Hayata at the National Institute of Subtropical Agriculture in Jeju island in 1992. The ‘'Jegam ga No. 2’', a first selection from the seedlings fruited in 2001 was finally named “"Haryejosaeng”" through field evaluation trials at three locations in Jeju island from 2003 to 2004. ‘'Haryejosaeng’' produces seedless fruit maturing in early November, and has higher soluble solids and lower acidity than ‘'Miyagawa Wase’', the leading early-maturing satsuma mandarin cultivar in Jeju island. Fruit weight is moderate at about 80~90 g and its shape is compressed-oblate globose with a light orange color. The rind thickness of about 2 mm provides easy peeling. The flesh shows light orange colored and contains 10 to 11 Brix and 1 to 1.1% acidity when mature. Tree shows vigorous growth and spreading thornless twigs. Alternate bearing is similar to ‘iyagawa Wase’ ‘aryejosaeng’is susceptible to citrus scab disease and melanose, but resistant to citrus canker.
‘'Hwangkeumnuri’' is a japonica rice variety developed and registered by the rice breeding team of Honam Agricultural Research Institute, NICS, RDA in 2006. This variety was derived from a cross between ‘'Milyang 165’' (Junambyeo) with good quality and high yield and HR14732-B-67-2-3 with multi-disease resistance. This variety has about 125 days growth duration from transplanting to harvesting in west-southern coast, Honam and Youngnam plain of Korea. It is about 76 cm in culm length and tolerance to lodging. In reaction to biotic and abiotic stresses, it shows resistance to blast, bacterial blight pathogen from K1 to K3 and stripe virus, but susceptible to other major diseases and insect pests. The milled rice of ‘'Hwangkeumnuri’' exhibits translucent, relatively clear non-glutinous endosperm and midium short grain. It has similar amylose content of 18.9% and lower protein content of 6.2%, and good palatability of cooked rice compared with ‘'Nampyeongbyeo’'. The milled rice yield performance of this variety is about 5.74 MT/ha in local adaptability test for three years. ‘'Hwangkeumnuri’' would be adaptable to west-southern coast, Honam and Yeongnam plain of Korea.
‘'Sinunbong 1’' is a japonica rice cultivar developed from the cross between “"Sangjubyeo”", high yield cultivar and ‘'Unbong 17’', cold tolerance at Honam Agricultural Research Institute (HARI), NICS, RDA, in 2005. This cultivar has a short grain shape and about 111 days growth duration from transplanting to harvesting in Korean climate condition. This cultivar shows high resistant reaction to the blast, but susceptible to bacterial blight and strip virus. This variety has cold tolerance, compared to Odaebyeo. The milled kernels of ‘'Sinunbong 1’' are translucent with non-glutinous endosperm. It has about 19.2% of amylose content and better palatability of cooked rice compared with ‘'Odaebyeo’'. The milled rice yield of ‘'Sinunbong 1’' is about 5.46 MT/ha under the standard fertilizer level of the ordinary transplanting cultivation. ‘'Sinunbong 1’' would be adaptable to the northern plain, mid-mountainous, southern mountainous of Korea.
A new spary chrysanthemum (Dendranthema grandiflorum) cultivar ‘Pinky’ was bred from a cross between ‘Rima’ and ‘Yen’. Progenies were investigated from 2004 to 2006 under shading culture in spring and retarding culture in autumn and the promising line was selected based on the performance of year-round production, white rust resistance and vase life. Pinky flowered around 20 October, and year-round production was possible by shading and lighting. Its flower was 3.1 cm in diameter, and had 38.4 petals and 26.8 flowers per stem in autumn. Its petal was narrow (0.4 cm) and pink with green in color at the flower center. Days to flower from short-day start for ‘Pinky’ was about 47 in spring. ‘Pinky’ showed a vase life of 20.9 days in autumn. This cultivar was commercially registered in 2007. ‘Pinky’ would be suitable for culture under the greenhouse and rain shelter condition in Korea.
‘'Morningpurple’' is a new sweetpotato variety developed by Mokpo Experiment Station, National Institute of Crop Science (NICS), RDA in 2007, for flowering. This variety was selected from the cross between Sinchunmi and Hi-dry in 2004, seedling and line selections were practiced from 2005 to 2006. ‘'Morningpurple’' has morning glory type flowers with purple color. It has triangular leaf, green with purple vine and petiole, elliptic storage root, red skin and yellow flesh color of storage root. The average yield of storage root was 15.0 ton/ha. Number of storage roots over 50 gram per plant was 2.6, and the average weight of storage root was 143 gram.