We investigated the standard cultivation substrate for Sparassis latifolia “Neowul” bred in Jeollabuk-do Agricultural Research and Extension Services. Cultivation characteristics and yield were assessed by varying the kind of sawdust and additives, and the mixing ratio. The cultivation period in larch sawdust was the shortest at 97 days. The yield was excellent (143.6 g). The findings indicated that larch is a tree species appropriate for the cultivation of S. latifolia. When the additives were varied, the yield and productivity (53.1%) were highest (116.6 g) for the wheat bran treatment. Thus, wheat bran would be an additive appropriate for culturing S. latifolia. Concerning the use of different mixing ratios, larch sawdust:beet-pulp:wheat bran ratios of 80:15:5 and 85:10:5 resulted in yields of 114.4 g and 111.4 g, and productivity of 52.5% and 51.8%, respectively. These yield and productivity values were not statistically different. Thus, the standard cultivation substrate for S. latifolia can comprise larch sawdust, beet pulp, and wheat bran at a ratio of 80:15:5 or 85:10:5. The carbon/nitrogen (C/N) ratio assumed to be appropriate for the cultivation of S. latifolia was 184 –223. Pinheading would be difficult below a C/N substrate ratio of 105. Thus, the C/N ratio of the media, as well as the pH, would be vital factors affecting pinheading during S. latifolia cultivation.

꽃송이버섯은 우리나라에서 2000년대 초반부터 재배를 시작하였고, 국내에서는 전라북도농업기술원이 육성한 ‘너울’이 2016년에 최초로 등록되어 있다. 그러나, 꽃송이버섯에 대한 재배매뉴얼이 없어 여전히 농가마다 자실체 생산성의 차이가 큰 실정이다. 이에 꽃송이버섯 ‘너울’의 발이조건을 구명하고 안정생산 기술을 개발하고자 배지 pH, 배지함수율, 종균접종량 조건에 따른 연구를 수행한 결과, 재배일수, 수확률과 자실체 중량을 고려한 연중 병당 수 량은 pH 3.8 처리구가 363.6 g으로 가장 높게 나타났으나 pH 3.6 조건에서는 수량이 189.5 g으로 급감하는 점, pH 4.0 조건이 배양일수가 짧고 pH 3.8 조건과 비교하여 재배일수가 같으며, 자실체 중량 차이의 유의성이 인정되지 않는 점, 농가에서 활용 시 정밀한 pH 조절이 어려운 점을 고려하여 pH 3.9±1로 조절하여 재배해야 안정생산이 가능할 것으로 판단된다. 아울러 배지함수율을 65%로 조절하고, 배지부피의 4%에 해당하는 액체종균을 접종 후 배양실 내 습도조건을 50% 이하로 설정하면 연중 병당 341.8 g을 생산할 것으로 판단된다.

Background: Visual and somatosensory integration processing is needed to reduce pusher behavior (PB) and improve postural control in hemiplegic patients with acute stroke. Objects: This study aimed to investigate the effects of game-based postural vertical training (GPVT) on PB, postural control, and activity daily living (ADL) in acute stroke patients. Methods: Fourteen participants with acute stroke (<2 months post-stroke) who had PB according to the Burke lateropulsion scale (BLS) (score>2) were randomly divided into the GPVT group (n1=7) and conventional postural vertical training (CPVT) group (n2=7). The GPVT group performed game-based postural vertical training using a whole-body tilt apparatus. while the CPVT group performed conventional postural vertical training to reduce PB (30 minutes/session, 2 times/day, 5 days/week for 3 consecutive weeks). The BLS was evaluated to assess the severity of PB. And each subject’s postural control ability and ADL level were assessed using the postural assessment scale for stroke (PASS), balance posture ratio (BPR), and Korean-modified Barthel index (K-MBI). Outcomes were measured preand post-intervention. Results: Comparison of the pre- and post-intervention assessment results showed that both interventions led to the following significant changes: decreased severity of PB scores and increased PASS, BPR, and K-MBI scores (p<.05). In particular, statistical analysis between the two groups, the BLS score was significantly decreased in the GPVT group (p<.05). And PASS, BPR, and K-MBI scores were significantly improved in the GPVT group than in the CPVT group (p<.01, respectively). Conclusion: This study demonstrated that GPVT lessened PB severity and improved postural control ability and ADL levels in acute stroke patients.

주름버섯목(Agricales), 소똥버섯과(Bolbitiaceae), 볏짚 버섯속(Agrocybe)에 속하는 버들송이버섯은 국내에서 대부분 병 재배용 품종으로 육성되어 있어 수량 증대를 위한 봉지재배용 품종을 개발하고자 본 연구를 수행하였다. 버들송이버섯 33개 유전자원을 수집하고 유전자원의 봉지재배 특성과 수량을 조사한 후 재배일수와 자실체 모양을 고려하여 5개 모본을 선발하고 3개의 교배조합을 작성 하여 7계통을 선발하였다. 선발된 7계통과 대조품종인‘참’을 봉지재배한 결과 재배일수는 ‘JBAC15-1’이 49일, ‘JBAC15-6’이 50일로 대조품종의 재배일수인 53일 대비 각각 4일과 3일이 단축되었으며, 그 외의 계통은 대조품 종보다 긴 재배일수를 나타내었다. 선발계통의 발이율은 ‘JBAC15-1’이 96.1%로 가장 우수하였으며 다음으로 ‘JBAC15-6’이 94.5%를 나타내어 대조품종의 발이율인 93% 대비 각각 3.1%, 1.5% 우수한 결과를 나타내었다. 봉지 당 수량은 ‘JBAC15-1’이 176.8 g으로 가장 우수하였고 ‘JBAC15-6’이 168.7 g으로 대조품종인 '참'의 160.7 g 대비 각각 10%, 5% 증수되었다. 이러한 결과를 토대로 대조품종인 ‘참’대비 재배일수가 짧고 수량이 우수한 ‘JBAC15-1’과 ‘JBAC15-6’을 최종 선발하였다. 선발계통 인 ‘JBAC15-1’, ‘JBAC15-6’의 균사생장 최적 배지는 PDA, 최적 온도는 27.5 o C, 최적 pH는 ‘JBAC15-1’이 pH 5, ‘JBAC15-6’이 pH 6으로 나타났다. 자실체 갓의 색은 ‘JBAC15-1’과 ‘참’이 진갈색을 나타냈으며 ‘JBAC15-6’ 은 연갈색을 나타내었다. 갓 형태는 반구형으로 모두 동일하였으나, 대의 색은 ‘JBAC15-1’이 연갈색을 나타냈으며 ‘JBAC15-6’은 흰색을 나타냈고 대조 품종인 ‘참’은 상아색으로 각각 서로 다른 특성을 나타내었다.

Agrocybe cylindracea was cultured in a bag, in which sawdust culture medium (1 kg) is put in a plastic bag (PE), with poplar sawdust, rice bran, wheat bran, and dried bean curd refuse in the ratio of 70:10:10:10 (v/v). 2% of the culture medium was inoculated with the liquid starter of Agrocybe cylindracea, and this was incubated at 25oC. After incubation, the A. cylindracea was further cultured by cutting the top vinyl portion of the bag down to the level of the culture medium surface of the first inoculation part. The cut culture medium was placed in a growth room at 25oC, and pin-heading was induced under light irradiation at 99% humidity and 1,000 ppm CO2 level for 3days. When the grow the environment was controlled at 95% humidity and 21oC, the bending of the stem was less as compared to that when the cap of the bag had been removed. The number of effective fruiting bodies per bag increased by 140% (28.8), the quantity per bag increased by 29.5%, and 148.5 g A. cylindracea could be potentially harvested.

본 시험은 추위에 강하고 생산성이 우수한 이탈리안 라이그라스 품종을 육성하기 위하여 2009년부터 2017년까지 국립축산과학원 초지사료과에서 수행되었다. 이탈리안 라이그라스 신품종 그린콜은 2배체 작물로 엽색은 녹색이며, 월동전 초형은 반직립형, 봄의 초형은 직립형이다. 그린콜은 4월 25일경에 출수하는 극조생종 품종이고 지엽폭이 8 mm, 지엽길이는 24.9 cm 이며, 출수기의 초장은 96 cm로 플로리다 80호 보다 5 cm 정도 짧다. 줄기 두께는 플로리다80호 보다 0.14 mm 굵고 이삭 길이는 플로리다 80호와 대등하다. 그린콜의 건물수량은 11,592 kg/ha로서 대조품종인 플로리다 80호에 비하여 약간 높았다. 상대적 사료가치는 그린콜이 96.7인데 비해 수입종인 플로리다 80호는 89.8로 다소 높고 가소화양분총량 (TDN)이 60.8 %로서 플로리다 80호에 비해 2.1% 높았고, 산성세제불용섬유 (ADF)와 중성세제불용섬유 (NDF)는 각각 35.5 및 58.9 %로서 대조 품종 보다 약간 낮았다.

Background: It is very difficult for hemiplegic patients to effectively perform the sit-to-stand (STS) movements independently because of several factors. Moreover, the analysis of STS motion in hemiplegic patients has been thus far confined to only muscle strength evaluation with little information available on structural and environmental factors of varying chair height and foot conditions. Objects: This study aimed to analyze the change in biomechanical factors (ground reaction force, center of mass displacement, and the angle and moment of joints) of the joints in the lower extremities with varying chair height and foot conditions in hemiplegic patients while they performed the STS movements. Methods: Nine hemiplegic patients voluntarily participated in this study. Their STS movements was analyzed in a total of nine sessions (one set of three consecutive sessions) with varying chair height and foot conditions. The biomechanical factors of the joints in the lower extremities were measured during the movements. Ground reaction force was measured using a force plate; and the other abovementioned parameters were measured using an infra-red camera. Two-way repeated analysis of variance was performed to determine the changes in biomechanical factors in the lower extremities with varying chair height and foot conditions. Results: No interaction was found between chair height and foot conditions (p>.05). All measured variables with varying chair height showed a significant difference (p<.05). Maximum joint flexion angle, maximum joint moment, and the displacement of the center of mass in foot conditions showed a significant difference (p<.05); however the maximum ground reaction force did not show a significant difference (p>.05). Conclusion: The findings suggest that hemiplegic patients can more stably and efficiently perform the STS movement with increased chair height and while they are bare-foot.

'Dakyeong' (Avena sativa L.), a winter oats for forage use, was developed by the breeding team at National Institute of Crop Science, RDA in 2016. It was derived from a cross between ‘CI7505’(IT133304) and ‘Swan’(IT197920). Subsequent generations followed by the cross were handled in bulk and pedigree selection programs at Iksan and Jeonju, respectively. After preliminary and advanced yield test for 2 years, ‘SO2004009-B-B-10-8-3-9’, designated as a line name of ‘Gwiri91’, were subsequently evaluated for earliness and forage yield during 3 years in four parts such as Jeju (upland), Yesan (upland), Iksan (upland), and Jeonju (paddy), from 2014 to 2016, and finally named as ‘Dakyeong’. Cultivar ‘Dakyong’ has leaves of dark green color, thick diameter culm and long grain of brown color. Over 3 years, the heading date of ‘Dakyeong’ was about 5 days earlier than that of check cultivar ‘Samhan’ (April 30 and May 5, respectively), and their average forage dry matter yield harvested at milk-ripe stage was higher 12% (15.7 tone ha-1) than 14.0 tone ha-1 of check cultivar. Cultivar ‘Dakyeong’ was lower than the check cultivar ‘Samhan’ in terms of the protein content (6.1% and 7.0%, respectively) and total digestible nutrients (62.1%, and 62.5%, respectively), while the TDN yield was more than the check (7.79 tone ha-1 and 7.64 tone ha-1, respectively). Fall sowing cropping of ‘Dakyeong’ is recommended only in areas where average daily minimum mean temperatures in January are higher than -6°C, and it should not be cultivated in mountain areas, where frost damage is likely to occur.

Oats (Avena sativa L.), which are known as one of the forage crops of Korea, have good livestock palatability and are popular to cattle farmers because of their high dry matter. However, the cultivation of double cropping in the rice field was reluctant due to the late maturing for farmers to plant rice continuously. 'Hi-early', a winter oats for forage use, was developed by the breeding team at National Institute of Crop Science, RDA in 2016. It was derived from a cross between ‘517A2-121’(IT133383) and ‘CI7604’ (IT133379). Subsequent generations followed by the cross were handled in bulk and pedigree selection programs at Suwon, Iksan and Jeonju, respectively. After preliminary and advance yield test for 2 years, ‘SO2004015-B-B-23-1-3-7’, designated as a line name of ‘Gwiri92’, were subsequently evaluated for earliness and forage yield during 3 years in four parts such as Jeju (upland), Yesan (upland), Iksan (upland), and Jeonju (paddy), from 2014 to 2016, and finally named as ‘Hi-early’. Cultivar ‘Hi-early’ has the characteristics of medium leaves of green color, thick diameter culm, and medium grain of brown color. Over 3 years, the heading date of ‘Hi-early’ was about 9 days earlier than that of check cultivar ‘Samhan’ (April 26 and May 5, respectively). Average forage fresh yield of ‘Hi-early’ harvested at milk-ripe stage was similar to check cultivar (40.2 tone ha-1 and 40.0 tone ha-1, respectively), and dry matter yield also was similar to check cultivar (14.2 tone ha-1 and 14.0 tone ha-1, respectively). Cultivar ‘Hi-early’ was lower than the check cultivar ‘Samhan’ in terms of the protein content (6.2% and 7.0%, respectively) and total digestible nutrients (61.0%, and 62.5%, respectively), while the TDN yield was more than the check (7.91 tone ha-1 and 7.64 tone ha-1, respectively). Fall sowing cropping of ‘Hi-early’ is recommended only in areas where average daily minimum mean temperatures in January are higher than -6°C, and it should not be cultivated in mountain areas, where frost damage is likely to occur.

In order to determine the possibility of artificial cultivation of Ganoderma lucidum, a study was carried out to optimize the medium composition used for bed culture. Sawdust spawn of medium composition for optimal growth was found to be the combination of 75% oak-sawdust, 10% cotton-seed meal, and 15% beet pulp. The optimal conditions for the formation of fruiting bodies were shown to be a temperature of 28–30oC, light intensity of 1,000–1,500 lux, and CO2 concentration of 1%.

봉지재배에 적합한 버들송이 신품종을 육성하고자 유전자원을 수집하고 재배 후 자실체 특성검정으로 우수자원(5계통)을 선발하였다. 선발자원으로부터 단포자 분리 및 교잡을 통해 JBAC15-1 등 35 교잡계통을 선발하였다. 35계통 중 PDA 평판배지를 이용하여 균사생장과 균총 밀도가 우수한 7계통을 1차 선발하였다. 톱밥 봉지재배 방식을 이용하여 선발 7계통의 재배특성을 조사한 결과, 재배일수가 50일 정도로 다른 계통에 비하여 짧고, 봉지 당 수량이 160g 정도로 높으며, 발이가 안정적인 3계통(JBAC15-1 계통 등)과 갓과 대 품질이 우수한 JBAC15-5 계통 등 총 4계통을 선발하였다. 선발계통의 rDNA ITS 영역 염기서열 분석 을 통한 유전적 다형성과 기존 품종과의 대치배양을 통해 신규성을 확인하였다. 평판배지를 이용한 선발계 통의 균 배양 특성으로, 배지종류는 PDA 배지에서 전반적으로 균사생장과 밀도가 우수하였고, 배양온도는 27.5°C에서 최대 생장 후 늦어지는 경향이었으며, 배지 pH는 5~7 정도의 약산성 배지에서 균 배양이 우수하였다.