법곤충학은 곤충과 관련된 법적 문제를 해결하는 학문으로써, 미국, 유럽, 한국 등 다양한 국가에서 범죄 수사, 사후경과시간 추정 등에 활용되고 있다. 한국은 최근, 법곤충감정실(경찰청) 개소하여 전국의 법곤충 사건을 감정하고 있다. 한국은 짧은 연구역사를 가짐에도 불구하고, 중앙행정기관 산하의 법곤충감정실을 설립, 운영할 수 있는 것은, 그 특이적인 연구역사에서 기인했다. 한국 법곤충 연구는 2004년경부터 시작되었으나, 중앙행정기 관(경찰청, 국립과학수사연구소, 농림축산검역본부)의 투자로 연구개발이 진행되었다. 특히, 한국의 경찰청은 2016-2020년과 2022-2026년, 10년간의 연구과제 발주를 통해 현장 중심의 법곤충 감정 시스템 구축, 비전문가를 위한 형태 및 분자 유전학적 종 동정 기법 개발, 한국형 DB 확보 등을 진행하고 있으며, 법곤충 감정기법의 세계적 인 선도를 목표하고 있다.

The Animal and Plant Quarantine Agency conducts a targeted sampling plan and analysis for veterinary drugs within the country every year. Target compounds included tetrachlorvinphos as an organophosphate, diminazene as an anti-infective medication, ketoprofen as a nonsteroidal anti-inflammatory drug, triclabendazole and clorsulon as flukicides in 2022. These compounds were not included in National Residues Program (NRP), despite their high sales ranking. A total of 94 bovine muscle samples and 20 equine muscle samples were collected from various locations across the country. The analysis of target compounds in muscle was performed using LC-MS/MS coupled with Food code 8.3.1 revised in 2022. A 2 g sample of muscle tissue was extracted using a water: acetonitrile (1:4, v/v) solution, then cleaned up with C18 and hexane saturated with acetonitrile. Compounds were separated with C18 column and mobile phases consisted of 0.1% formic acid in water (A) and 0.1% formic acid in acetonitrile (B). All analytes exhibited good linearity with correlation coefficients (R2) higher than 0.992. The limit of quantification (LOQ) of these compounds ranged from 0.21 to 2.79 μg/kg except for diminazene (3.85~6.86 μg/kg). The average recoveries of these analytes were 89.45~129.13% in muscle at spiked level of 10 or 20 μg/kg. Relative standard deviations (%) (intra-day and inter-day) were lower than 20% for all target compounds, except for diminazene and triclabendazole, whose intra-day RSD % was slightly higher than 20% in equine muscle. Testing confirmed that all 94 bovine and 20 equine muscle samples from 9 provinces were free from residues of veterinary drugs. Monitoring of compounds not included in the NRP should continue to ensure consumer health and food safety.

This study introduces the licensing process carried out by the regulatory body for construction and operation of the 2nd phase low level radioactive waste disposal facility in Gyeongju. Also, this study presents the experience and lessons learned from this regulatory review for preparing the license review for the next 3rd phase landfill disposal facility. Korea Radioactive Waste Agency (KORAD) submitted a license application to Nuclear Safety and Security commission (NSSC) on December 24, 2015 to obtain permit for construction and operation of the national engineered shallow land disposal facility at Wolsong, Gyeongju. NSSC and Korea Institute of Nuclear Safety (KINS) started the regulatory review process with an initial docket review of the KORAD application including Safety Analysis Report, Radiological Environmental Report and Safety Administration Rules. After reflecting the results of the docket review, the safety review of revised 10 application documents began on November 29, 2016. Total 856 queries and requests for additional information were elicited by thorough technical review until November 16, 2021. As the Gyeongju and Pohang earthquakes occurred in September 2016 and November 2017, respectively, the seismic design of the disposal facility for vault and underground gallery was enhanced from 0.2 g to 0.3 g and the site safety evaluation including groundwater characteristics was re-investigated due to earthquake-induced fault. Also, post-closure safety assessments related to normal/abnormal/human intrusion scenarios were re-performed for reflecting the results of site and design characteristics. Finally, NSSC decided to grant a license of the 2nd phase low level radioactive waste disposal facility under the Nuclear Safety Laws in July 2022. This study introduces important issues and major improvements in terms of safety during the review process and presents the lessons learned from the experience of regulatory review process.

This study aims to establish a modified analytical method with sensitivity and reliability for streptomycin (STP) and dihydrostreptomycin (DHS) of residues level in pig tissues, plasma and urine by LC-MS/MS on the basis of previous studies. The mass parameters of quantitative and qualitative ions for STP and DHS were optimized using multiple reaction monitoring in positive mode. The separation of compounds was conducted using BEH Amide column according to material’s characteristics. The analytes in plasma were extracted with only organic solvents. In muscle and kidney, KH2PO4 buffer solution containing 2% CCl3COOH and EDTA-Na was used as extraction solvent. The WCX cartridege was selected as SPE cartridge in considering high recoveries for STP and DHS. The analytes in urine were extracted by organic solvents with acid and addition of EDTA. The limits of detection (LODs) in STP and DHS ranged 0.45~3.66 μg/kg and 0.22~0.78 μg/kg, respectively. The limits of quantification (LOQs) were 1.35~11.10 μg/kg in STP and 0.66~2.36 μg/kg in DHS. The recoveries (%) were 94.29~104.5% in STP and 92.32~108.45% in DHS except for plasma with lower values (61.45/68.5%, respectively). In the precision evaluation, the coefficient of variation (CV, %) of STP showed <10.50% on intra-day and <18.04% on inter-day. The CV (%) of DHS showed <8.42% on intra-day, whereas <17.98% on inter-day. The modified method is reliable for continuous residual monitoring in pig to ensure food safety for consumer’s health. In addition, this method could be used in study relation to residue depletion and pharmacokinetics of veterinary drug.

In this study, the near-complete genome sequence of the novel reassortant H1N2 influenza A virus strain A/swine/Korea/KS60/2016 is reported. Sequences of the hemagglutinin (HA), neuraminidase (NA), and polymerase basic 2 (PB2) genes were analyzed, revealing that the isolates contain segments from previous Korean swine H1N2 strains. Additionally, the remaining genes of this strain originated from human H1N1 strains in 2009.

Embryonic lethality due to recessive alleles is a major concern in livestock breeding programs. The Online Mendelian Inheritance in Animal (OMIA) is a database of reported recessive mutations in livestock that helps breeders to manage the segregation of these mutations in their population. Recessive alleles are lethal in the offspring of two carrier parents; therefore, identifying and eliminating carrier animals are critical for maintaining the breed. Hanwoo cattle is native to the Korean peninsula and is of great economic importance to Korea. Due to geographical constraints and the controlled breeding program with very few proven bulls, if not monitored periodically, the threat of segregation of recessive lethal alleles in the population remains high. Therefore, identifying potential carriers of lethal recessive alleles is critical in Hanwoo. In this regard, we genotyped 17,325 animals using bovine 50K Illumina SNP chip and also sequenced a further 311 animals which was mapped against the bovine reference genome sequence. We then used the OMIA database to identify reported recessive alleles and calculated the allele frequency of these mutations in the population to determine potential lethal alleles carried by the animals.

우간다에서 쌀은 식량, 농가소득 및 안보를 위한 전략적 작물로 목화, 커피, 옥수수에 이어 우간다의 농업 전략 계획 (ASSP)에서 우선순위12개 작물 중 4번째로 선정될 정도로 매우 중요한 작물이다. 쌀은 생산 측면에서 옥수수에 이어 두 번째로 중요한 곡물이다. 최근 우간다에서는 쌀 소비가 빠르게 증가함과 동시에 매년 쌀 수입 또한 비례적으로 빠르게 증가하고 있다. 쌀의 소비 증가는 우간다 쌀 생산량의 90%를 차지하는 소규모 재배 농민들에게 엄청난 경제적 기회를 제공하고 있는데 이는 쌀은 다른 곡물에 비해 상대적으로 훨씬 높은 금액으로 판매되고 이익 또한 높기 때문에 현금 작물로서 벼 재배 면적이 빠르게 증가하고 있는 실정이다. 그러나 벼 생산성은 여전히 낮은 수준인데, 이는 주로 수확량이 적은 쌀 품종의 사용, 개선 된 품종의 종자에 대한 농부의 접근 제한, 낙후된 재배 기술, 비료 및 농약 사용이 매우 저조하기 때문이다. 우간다의 쌀 재배 현황과 여건에 대하여 조사한 바는 아래와 같이 정리할 수 있다. 1. 쌀 생산을 위한 기후적 여건: 연평균 강수량은 1,180 mm 정도이고 연평균 기온은18도에서 35도 사이로 벼 재배에 좋은 기후적 여건이다. 2. 주요 재배 지역: 우간다에는 3곳의 주요 벼 재배 중심지역이 있다. 동쪽 지역은 주로 관개시설과 강수에 의존하는 밭 벼를 재배하고 있으며, 북쪽과 중서부 지역은 논벼를 주로 재배하고 있다. 우간다 쌀 생산량의 90%를 차지하는 소규모 재배 농민들은 주로 위의 3지역에서 벼를 생산하고 있다. 3. 벼 생산성에 영향을 미치는 요소들: 제한적 관개시설과 기계화, 그리고 양질의 종자 부족과 낮은 수준의 농업기술 등이 낮은 생산성의 주요 요소들이며, 수확 후 관리 기술과 저 장 시설 부족, 충분하지 못한 재정적 지원 그리고 병해충 등의 요소들이 있다. 4. 주요 벼 품종: 우간다 현지에서 주로 재배되는 벼 품종은 9종으로서 다음과 같다. Namche, Komboka, Kaiso, Wita 9, Basmat 370, IR 64, Supa, Buyu 및 NERICA 품종이다. 5. 농촌진흥청 한-아프리카 농식품 기술협력 협의(KAFACI)를 통해 높은 생산성과 질병 저항성을 목적으로 개발 후 육종 된 두 한국 품종(KAF-172-67과 KAF-304-287)은 우간다에서 등록절차가 진행될 예정이다.

Porcine parvovirus 2 (PPV2) was recently detected in the Republic of Korea. This paper reports two near-complete genome sequences of PPV2 identified for the first time in the lung tissue of aborted pig fetuses.