Low temperature stress is one of the major negative factors affecting vegetative and reproductive growth of rice. To better understand responses of rice plants to low temperature we analyzed transcriptome expression patterns in glumous flower of cold-tolerant japonica rice variety, Stejaree45, and cold-susceptible variety, HR19621-AC6 at booting stage under cold water irrigation. A total of 2,411 probes were differentially expressed by low temperature in glumous flowers of the two varieties. Some important genes involved in hormone biosynthesis showed variety-specific regulation. Expression of GA20ox3 and GA2ox, among the genes involved in GA biosynthesis, was regulated differentially in the two varieties. Among the genes involved in IAA biosynthesis, YUCCA1 and TAA1:1 showed variety-specific regulation. Among the genes involved in cytokinin biosynthsis and signaling, expression of LOG, HK1 and HK3 was significantly down-regulated only in the cold-susceptible variety. Among the genes involved in ABA biosynthesis, NSY and AAO3 were down-regulated only in the cold-tolerant variety. In general, genes involved in GA, IAA and cytokinin biosynthesis responded to cold temperature in such a way that capacity of those bioactive hormones is maintained at relatively higher levels under cold temperature in the cold-tolerant variety, which can help minimize cold stress imposed to developing reproductive organs in the cold-tolerant variety.
In this study, 15 simple sequence repeat (SSR) markers were used to analyze the population structure of 55 mungbean accessions (34 from South Asia, 20 from West Asia, 1 sample from East Asia). A total of 56 alleles were detected, with an average of 3.73 per locus. The mean of major allele frequency, expected heterozygosity and polymorphic information content for 15 SSR loci were 0.72, 0.07 and 0.33 respectively. The mean of major allele frequency was 0.79 for South Asia, and 0.74 for West Asia. The mean of genetic diversity and polymorphic information content were almost similar for South Asian and West Asian accessions (genetic diversity 0.35 and polymorphic information content 0.29). Model-based structure analysis revealed the presence of three clusters based on genetic distance. Accessions were clearly assigned to a single cluster in which >70% of their inferred ancestry was derived from one of the model-based populations. 47 accessions (85.56%) showed membership with the clusters and 8 accessions (14.54%) were categorized as admixture. The results could be used to understanding the genetic structure of mungbean cultivars from these regions and to support effective breeding programs to broaden the genetic basis of mungbean varieties.
To investigate dose-effect of a chemical mutagen, sodium azide on a rice elite line, Suweon472, seed aliquots were treated with five different concentrations of sodium azide. The degree of mutation levels of each aizde concentration were estimated by using DNA fingerprinting techniques such as RAPD and AFLP. Some selected mutant lines (M4) were also subjected for DNA fingerprinting to estimate their mutation levels by comparing the banding patterns of the wild type, Suweon 472. RAPD and AFLP fingerprinting patterns indicated that dose-effect of different azide concentrations was not clear. With allele description of detected AFLPs among favorable mutant lines, it was possible to discriminate each mutant line from others which have similar phenotypes and reactions against pathogens. AFLP fingerprinting patterns of waxy mutant lines, otherwise, were highly homogeneous as well as their phenotypic and agronomic characters.
The objective of this study was to determine the fatty acid composition of newly developed tissues of germinated soybean seeds. Five soybean accessions with varied fatty acid composition were allowed to germinate in sand under greenhouse conditions. Seedlings were picked up after 4, 6, 8, 10 and 12 days of germination and freeze dried. The fatty acid composition of the newly developed tissues was analyzed by gas chromatography. Significant variation in fatty acid composition was observed between accessions, days of germination, and variety × day of germination in whole and the cotyledons. In the case of newly developed five tissues, significant variation in fatty acid composition were observed between days of germination except oleic acid for root, hypocotyl and epicotyl stem and except stearic acid for hypocotyl and unifoliate leaves while all the parameters were significantly different for accession. Significant interactions of accession and days of germination were observed for palmitic, linoleic and linolenic acid in all tissues; only for oleic acid in hypocotyl, epicotyl and unifoliate leaves; and only for stearic acid in root, hypocotyl, epicotyl and unifoliate leaves. During germination, the fatty acid composition of newly developed tissues changed dramatically but whole seedlings and cotyledons changed slightly. These tissues contained five major fatty acids as found in original seeds, but compositions were totally different from that of the seed: higher in palmitic, stearic and linolenic acid and lower in oleic and linoleic acid. New tissues conserved their fatty acid compositions regardless of genotypic variation in the original seeds.
본 연구는 모로베레칸의 잎도열병 저항성유전자를 탐색하고, 이 저항성유전자와 연관된 분자표지를 탐색하기 위해 수행되었다. 도열병에 이병성인 일품벼와 도열병에 강한 모로베레칸을 교잡하여 육성된 140개 BC3F3 계통을 도열병 균계 반응을 통한 저항성 유전자 탐색에 이용하였다.
반수체 육성은 육종연한을 단축시키기 위해 유용한 도구이다. 본 연구는 밀 품종 조기 육성을 위한 반수체 육종 시스템을 개발하기 위해 반수체 육성시 식물생장조절제 처리가 배 배양에 미치는 영향에 대해서 조사한 것이다. 밀 이삭의 제웅 작업을 수행하고 옥수수와 원연교잡한 후 종자결실은 2,4-D 및 2,4,5-T 150 mg/L, dicamba 50 mg/L 처리에서 가장 좋았으며, 배형성은 2,4-D 및 2,4,5-T 100 mg/L, dicamba 50
벼멸구 저항성 Bph18 유전자를 가진 SR30071-3-7-23-6-1-1-1계통과 감수성인 '새누리'를 교배하여 육성한 약배양집단 192계통에 대해 벼멸구 저항성 유전자 Bph18이 벼의 수량구성요소에 미치는 영향을 검토하였다. 벼멸구 저항성 유전자 Bph18과 밀접히 연관된 7312.T4A 마커의 유전자형을 분석한 결과, 저항성 유전자형을 가진 계통은 24계통이었고 감수성 유전자형을 가진 계통은 168계통이었다. 벼멸구 저항성 유전자 Bph18은
식용 찰옥수수에 대해 소비자의 기호성과 기능성이 고루 높고 가공적성이 우수한 복합 신품종을 육성하고자 현재 재배되고 있는 장려품종을 대조구로 하여 총 15개 교잡종을 아밀로그램 특성, 항산화성 및 식미관련 종실특성을 분석한 결과는 다음과 같다. 1. 공시된 교잡종에 대한 아밀로그램 분석결과 식미에 유리한 최고 점도 및 강하 점도는 CNU08H-69 교잡종에서 각각 115.9와 53.8RVU로 가장 높았고 CNU08H-h121과 일미 찰이 각각 51.6와
열대지역에 적응하는 온대 자포니카 벼 품종 개발을 위한 이삭형질 개선의 육종 방향을 제시하고자 한국과 필리핀 우기재배에서의 이삭형질 변이 및 특성에 대해서 조사하였다. 이삭형질의 변이는 2차 지경 착생립수가 차이가 가장 많이 나는 형질이었고 1차 지경 평균립수가 가장 적게 나는 형질이었다. 2차 지경 관련 형질이 1차 지경 관련 형질보다 수당립수와의 상관관계가 높았다. 지역간 차이가 많이 난 형질은 2차 지경 립수, 2차 지경수, 수당립수, 총지경수 순
야생벼은 재배벼의 친환경적성을 강화시킬 수 있는 병해충 저항성 및 불량환경에 견딜 수 있는 유용한 유전자들의 보고로 알려져 왔다. 국내에서 육성된 벼 품종인 '화성'(AA게놈)와 야생벼인 Oryza. minuta(BBCC 게놈; Acc.=101141)간의 교잡을 통하여 종간잡종 후대들이 육성되었다. 불화합성과 초기분리세대의 극심한 불임을 극복하기 위해 배주배양으로 F1 개체를 확보하였으며, '화성'으로의 여교잡을 수 차례 실시하였다. 확립된 계통들에 대