Isoflavone is synthesized in several different organs such as root, leaf and developing and germinating seed. Isoflavone content reflects overall history of physiological event given to a plant during certain period of time. Amount of isoflavone in a orga
This study was conducted to assess genetic diversity among 16 genotypes of boxthorn (Lycium chinensis Mill.) using inter-simple sequence repeat (ISSR) markers. The 18 ISSR primers out of 100 primers showed the amplification of 101 reproducible fragments. A total of 56 DNA fragments were polymorphic with an average 3.1 polymorphic bands per primer. The polymorphic primers were divided into 16 anchored primers and 2 non-anchored primers. All of the anchored primers were di-nucleotide repeat motif, and polymorphism level was higher in the primers with poly GA and CT motif than CA and GT motif primers. Based on polymorphism, cluster analysis was conducted by the unweighted pair-group method with arithmetic average (UPGMA) methods. Sixteen boxthorn varieties and accessions were separated into 2 distinctive groups and genetic distance of cluster ranged from 0.82 to 0.97. Eighteen markers were able to distinguish every variety. Therefore, ISSR markers may be suitable for characterizing the large numbers of germplasms and fingerprinting of boxthorn varieties.
Total of 69 open-pollinated family seeds were harvested from the clonal seed orchard of Pinus thunbergii, and planted at Jeju and Anmyon in 1992. When the progenies were 16 years old, height, DBH and volume growth were measured. The results showed that al
The sprout characteristics for 783 sprout soybean lines screened by pure line selection method were evaluated. Those developed lines were originally collected on farmers fields all around the country. Some of the lines showed higher yield rateof soybean
Ostrinia furnacalis Guenee is the most important pest of Zea may L. Little is known about combining ability for stem damage to this insect. The objective of this study was to estimate of resistance to stem damage by the Oriental corn borer. The importance
This study was carried out to evaluate the variation of physiological nitrogen-use efficiency (PNUE: Grains yield/total nitrogen content of shoot) in rice under different nitrogen (N) fertilizer levels, and to investigate the relationship of PNUE with agr
Twenty-five promising lowland rice genotypes of eastern India were studied for genetic variability in twelve quality characters and grain yield. Analysis of variance for all the characters indicated significant differences among genotypes for all the phys
Saponin and ginsenosides are very important as medicinal components of ginseng. These components are significantly different according to Panax species, cultivation area, or manufactured products. In this research, we comparatively analyzed the characteri
Oil and fatty acid composition of 648 soybean germplasms of different categories including Korean and American source were analyzed by NIRS (Near Infrared Reflectance Spectrophotometer) method at Yeongnam Agricultural Research Institute, Milyang, Korea. A
Male and female strobilus production was assessed annually over a five-year period for a clonal seed orchard of sugi (Cryptomeria japonica D. Don) in Korea. Clonal fertility and fertility variation, expressed by both sibling coefficient and coefficient of variation in strobilus production among 40 orchard clones, were reported. Fertility varied among clones and among years, producing five-year averages per ramet of 464.3 and 971.1 for female and male strobili, respectively. The correlation between female and male strobilus production was positive in each of the five years studied and statistically significant with the exception of one poor flowering year. The clonal status number (Ns), a measure of genetic diversity, was calculated based on the observed clonal fertility variation, varying from 27.6 (N = 40) in the poorest flowering year (2002) to 35.8 in the best year (2005). On average (pooled), the relative status number was 95% of the census number (N). Variation of female fertility was higher than that of male fertility for the five consecutive years, and the status numbers of female and male parents reflected this variation. The pooled Ns estimation from all five years was higher than any single year, implying that genetic diversity would increase when seeds collected from different years are pooled. Sexual asymmetry calculations showed that clonal contributions would be balanced between genders
A reaction of 214 lines and varieties to BB korean race K1, resistance and moderately resistance was 33 varieties which were bred before in 1990, susceptible were 5 varieties (Milyang23, Dongjinbyeo, Sinseonchalbyeo, Changseongbyeo and Cheongmyeongbyeo). Among bred after in 1990 resistance appeared 58 varieties, 18 varieties were susceptible. Bred after in 2000 were 50 varieties appeared resistance and 5 varieties were susceptible (Manho, Goun, Jinbong, Josaengheugchal, and Manna). Accordingly most varieties bred in south korea seemed to possession resistance to BB korean race K1. As a result of whether or not having Xa1 gene of rice varieties by SNP marker appeared that 43 varieties and the other 10 lines and varieties have Xa1 gene. Varieties bred before in 1990 have 18 (62.1%) varieties out of 29 varieties possession Xa1 gene to showed resistance to BB korean race K1, 19 (47.5%) varieties bred in 1990~2000, 6 (12.8%) varieties (Boseogchal, Daepyong, Hanareumbyeo, Hopyeongbyeo, Jongnam, Kuman) bred after in 2000 seemed to possession Xa1 gene.
The 5S rRNA gene is considered as valuable resource for chromosome landmarks and evolutionary studies. In this study, the tandem repeat unit of 5S rRNA gene containing the coding sequence and non‐transcribed spacer (NTS) region was amplified by PCR, cloned, and sequenced from Allium wakegi (2n=XY=16) and the two ancestors, A. cepa and A. fistulosum. Although there were intraspecific variations among the clones of each species, we could construct each consensus sequences for A. cepa and A. fistulosum and two consensus sequences for A. wakegi. The sequence of the 120 bp coding region was completely homologous among the consensus sequences from the Allium species examined. However, the sequence in the NTS region was significantly variable and informative in genome analysis. The variations were found to be clustered between the positions 230 and 269, and also distributed broadly as single nucleotide. A. wakegi could be divided into two classes by the 5S rRNA sequences (AWAK‐1 and AWAK‐2). AWAK‐1 showed high homology to the genome of A. cepa and AWAK‐2 to that of A. fistulosum. Fluorescence in situ hybridization technique was applied to analyze the distribution of 5S gene loci. In A. wakegi, 5S rRNA sequences were detected in two different chromosomes, each one showing the pattern identical to the chromosome donated from each genome of both ancestors.
The direct use of mutation is a valuable approach to generate variability in crops. The electron beam, one of the ionizing radiations, has been applied to evaluate its effect on seed germination and early seedling growth of creeping bentgrass (Agrostis palustris Huds., cv Penn-A1). The mature dry seeds were irradiated with various electron beam energies (0.3, 1.0, 1.3, and 2 MeV) and current levels (0.03 and 0.06 mA). Although large variability was existed within each dose, distinct difference of germiability and seedling vigor were not found at 0.3 MeV / 0.03 mA and 0.3 MeV / 0.06 mA beam condition. However, 1.0 MeV / 0.06 mA application most effectively inhibited and retarded seed germination and most severely restricted cotyledon and root growth in early seedling growth. The direct use of electron beam would be a valuable supplementary approach to generate mutants suitable for breeding purposes.
A new sprout-soybean cultivar, “Wonhwang” was developed at the Honam Agricultural Research Institute (HARI) in 2005. Wonhwang was selected from a cross between Camp and Myeongjunamulkong. The preliminary, advanced, and regional yield trials for evaluation and selection of Iksan45 were carried out from 2001 to 2005. This cultivar has a determinate growth habit with purple flower, grayish brown pubescence, grayish brow hilum, lanceolate leaflet shape and small seed size (10.0 grams per 100 seeds). The maturity date of Wonhwang is 7 days earlier than that of the check variety, Pungsan. It has good seed quality for soybean-sprout, and resistance to lodging. It has also been identified to have resistance to soybean mosaic virus (SMV) and necrotic symptom(SMV-N). The average yield of “Wonhwang” was 2.80MT/ha, which was higher by 4% than “Pungsannamulkong” at the regional yield trials.
“Ahyun”, an Asiatic lily cultivar was released by the National Horticultural Research Institute (NHRI), in 2001. A crossing was made between Asiatic lily “Make up”, a spotted light pink colored variety, and “Anabelle”, a spotted deep orange variety in 1993. It was selected in natural cultivation condition from the progenies of a cross combination between “Make up” and “Anabelle” in 1995. The flower characteristics and performance of “Ahyun” were investigated from 1999 to 2001. The natural flowering time of “Ahyun” was late June, but year-round cut flower production was possible by storing the bulbs under -1 to -2℃ conditions. It has light orange petals with a little spots. The plant height is 104 cm. The width of flower is 15.6 cm. The weight and circumstance of bulb are 41.3 g and 14.8 cm, respectively. For forcing culture, it is necessary to add calcium to the fertilizer or remove side scales to prevent leaf scorch. Botrytis disease should be properly controlled especially in wet season.
A new yellow gerbera (Gerbera hybrida Hort.) cultivar “Gold Eye” was developed from a cross between “Songsongee” and “White Day” followed by seedling and line selections at the Flower Breeding Research Institute, Gyeongnam Agricultural Research and Extention Services (ARES) in 2001. Characteristic trials were conducted three times from 2003 to 2004. Newly bred “Gold Eye” have shown yellow color (RHS, 55-C), semi-double type, and large flower. It also was in good harmony in ray floret color and flower center color with stable flower shape. The vase life was 10.2 days. The average yield of “Gold Eye” was 48.8 flowers per plant in an year in greenhouse yield trails during 2003 and 2004. This cultivar was registered for a commercialization in 2005. “Gold Eye” would be cultured under greenhouse condition in Korea.
A new pink gerbera (Gerbera hybrida Hort.) cultivar “Pink Giant” was developed from a cross between “Salina” and “Marita” followed by seedling and line selections at the Flower Breeding Research Institute, Gyeongnam Agricultural Research and Extention Services (ARES) in 1999. Characteristic trials were conducted three times from 2002 to 2004. Newly bred “Pink Giant” have shown pink color (RHS, 65-B), semi-double type, and large flower. It also was in good harmony in ray floret color and flower center color with stable flower shape. The vase life was 19.2 days. The average yield of “Pink Giant” was 45.8 flowers per plant in an year in greenhouse yield trails during 2003 and 2004. This cultivar was registered for a commercialization in 2005. “Pink Giant” would be cultured under greenhouse condition in Korea.
A new chestnut cultivar, “Mipung” was released from Korean native chestnut trees by chestnut laboratory of the Korea Forest Research Institute (KFRI) in 2006. This cultivar was selected first from a natural population in 1984, and the preliminary, advanced, and regional yield trials for evaluation were carried out from 1985 to 2005. “Mipung” is a late-ripening cultivar, and its optimal ripening date is late September. It has good nut quality for eating raw and processing. Its average nut weight is 27.3 g, much heavier than that of the check cultivar, “Arima”. It had also been identified to have resistance to chestnut blight disease and chestnut gall wasp threatening to chestnut trees. The average yield of 8 year-old “Mipung” was 8.8kg per tree, which was superior to that of “Arima”.
“Bora” is a new perilla (Perilla frutescens (L.) Britton) cultivar developed from a cross between Ipdeulkkae1 and YCPL199 at National Yeongnam Agricultural Experiment Station, RDA, in 2001. Purple backside leaf color is one of the most important characteristics for the high quality perilla leaf. “Bora” has purple backside leaf color in all climate condition, while a check variety, Ipdeulkkae1 has too light color during summer season or in high temperature condition. “Bora” matures at October 18th and has 79 cm of the stem length, shorter than that of Ipdeulkkae1. Although leaf yield is 11% less than that of a check variety Ipdeulkkae1, the harvested leaf number of “Bora” is similar with that of Ipdeulkkae1. The cultivar is appropriable in all area of Korea for the leaf harvesting cultivation whereas it is restricted to southern part of Korea South Gyeongsang and South Jeola provinces, for the seed harvesting cultivation.
“Ilyeop” (Perilla frutescens (L.) Britton), a new cultivar for leaf vegetable, was developed from a cross between Ipdeulkkae1 and YCPL187 at the National Yeongnam Agricultural Experiment Station, RDA, in 2001. For the cultivation of leaf vegetable perilla, the plants are grown with a spacing of 10 × 5 cm between rows and plants and all branches are removed to harvest uniform leaves from main stem, indicating that the amount of the next harvesting leaf is a important characteristic for the growth of the plant. “Ilyeop” grows vigorously and yields high quality leaf with higher amount of the next harvesting leaves composed with a check cultivar, “Ipdeulkkae1”. “Ilyeop” has five days of earlier seed maturity (Oct. 24) than that of “Ipdeulkkae1” under natural condition, less chance of frost damage is expected. The flowering date of “Ilyeop” is same with that of “Ipdeulkkae1” in winter cultivation with night break treatment.