This study aimed to prepare kombucha, a fermented tea beverage, containing Dendropanax morbiferus (DM) leaves and roots, and analyze its antioxidant and intracellular activities. We compared the pH change, total acidity, radical scavenging activity, and oxygen radical absorbance capacity (ORAC) of kombucha fermented with black tea alone and that with added DM leaves or roots during fermentation. Using RAW 264.7, we evaluated the effects of kombucha containing different DM parts on nitric oxide (NO) production and inflammation-related cytokine content in cells. Kombucha containing ethanol extracts of DM leaves (BTK-E-DML) and roots (BTK-E-DMR) showed higher radical scavenging activity and ORAC 3 d after fermentation than that prepared from black tea alone (BTK-Ori). In an in vitro experiment using RAW 264.7, samples were treated with 8 mg/mL kombucha considering cytotoxicity; the lipopolysaccharide (LPS)-induced NO content significantly reduced after BTK-E-DML and BTK-EDMR treatments compared with that after BTK-Ori treatment. Additionally, the levels of interleukin-6 and tumor necrosis factor-alpha, which were LPS-stimulated inflammatory cytokines, significantly decreased in cells treated with BTK-E-DML and BTK-E-DMR 15 d after fermentation compared with those treated with BTK-Ori. In conclusion, these results demonstrate that kombucha fermented with the leaves and roots of DM increases antioxidant activity and can significantly regulate inflammatory responses at the cellular level.

Alpha activities can be used for categorization, transportation, and disposal of radioactive waste generated from the operation of nuclear facilities including nuclear power plants. In order to transport and dispose of such low- and intermediate-level radioactive waste (LILW) to the Wolsong LILW Disposal Center (WLDC) at Gyeongju, the gross alpha concentration of an individual drum should be determined according to the acceptance criteria. In addition, when the gross alpha concentration exceeds 10 Bq/g, the inventory of the comprising alpha emitters in the waste is to be identified. Gross alpha measurements using a proportional counter are usually straightforward, inexpensive, and high-throughput, so they are broadly used to assay the total alpha activity for environmental, health physics, and emergency-response assessments. However, several factors are thoughtfully considered to obtain a reliable approximate for the entire alpha emitters in a sample, which include the alpha particle energy of a particular radionuclide, the radionuclide that is used as a calibration standard, the uniformity of film in a planchet, time between sample collection and sample preparation, and time between sample preparation and counting. Korea Atomic Energy Research Institute (KAERI) have evaluated the inventory of radionuclides in low-level radioactive waste drums to send every year hundreds of them to the WLDC. In this presentation, we revisit the gross alpha measurement results of the drums transported to WLDC in the past few years and compare them with the concentrations of alpha emitters measured from alpha spectrometry and gamma spectrometry. This study offers an insight into the gross alpha measurement for radioactive waste regarding calibration source, self-absorption effect, composition of alpha emitters, etc.

The physicochemical similarities of hydrogen isotopes have made their separation a challenging task. Conventional methods such as cryogenic distillation, Girdler sulfide process, chromatography, and thermal cycling absorption have low separation factors and are energy-intensive. To overcome these limitations, research has focused on kinetic quantum sieving (KQS) and chemical affinity quantum sieving (CAQS) effects for selective separation of hydrogen isotopes. Porous materials such as metal-organic frameworks (MOF), covalent organic frameworks (COF), zeolites, carbon, and organic cages have been studied for hydrogen separation. In this study, we focus the enhancement for CAQS to provide the cations due to the chemical affinity between hydrogen isotope and unsaturated sites by cations in zeolite beads. Cation exchanged zeolite beads was synthesized with cobalt, copper, nickel, iron and silver in zeolite 4A beads. Synthesized cation exchanged zeolite was analyzed for the surface area and pore size in N2 and adsorption behaviors of hydrogen isotopes (D2/H2) for various cation exchanged zeolite beads using BET at 77 K. The study predicts the D2/H2 adsorption selectivity based on the results obtained with BET. These hydrogen isotope adsorption results will provide a foundation for future processes for tritium separation.

Spent ion exchange resins have been generated during the operation of nuclear facilities. These resins include radioactive nuclides. It is needed to fabricate them into a stable form for final disposal. Cement solidification process is a useful method for the fabrication of them into a waste form for final disposal. In this study, proper conditions for the fabrication of them into a stable waste form were determined using the cement solidification process. In-drum waste forms were then produced at the conditions, where the stability of representative samples was evaluated for final disposal. The samples were satisfied to the Waste Acceptance Criteria for low and intermediate level radioactive waste disposal sites. This result can be utilized to derive optimal conditions for the fabrication of spent ion exchange resins into a final disposal form.

Advanced countries in the field of nuclear research and technology are currently examining the feasibility of deep geological disposal as the most appropriate method for the permanent management of high-level radioactive waste, with no intention of future retrieval. Deep geological disposal involves the placement of such waste deep underground within a stable geological formation, ensuring its permanent isolation from the human environment. To guarantee the enduring isolation and retardation of radionuclides with half-lives spanning tens of thousands to millions of years from the broader ecosystem, it is imperative to comprehend the long-term evolution of deep disposal systems, especially the role of natural barriers. These natural barriers, typically consisting of bedrock, encase the repository and undergo long-term evolutions due to tectonic movements and climate variations. For the effective disposal of high-level radioactive waste, a thorough assessment of the site’s long-term geological stability is essential. This necessitates a comprehensive understanding of its tectonic evolution and development characteristics, including susceptibility to seismic and magmatic events like earthquakes and intrusions. Furthermore, a detailed analysis of alterations in the hydrogeological and geochemical environment resulting from tectonic movements over extended time frames is required to assess the potential for the migration of radionuclides. In this paper, we have examined international evaluation methodologies employed to elucidate the predictive long-term evolution of natural barriers within disposal systems. We have extracted relevant methods from international case studies and applied a preliminary scenario illustrating the long-term evolution of the geological environment at the KURT (KAERI Underground Research Tunnel) site. Nevertheless, unlike international instances, the scarcity of quantitative data limits the depth of our interpretation. To present a dependable scenario in the future, it is imperative to develop predictive technologies aimed at comprehensively studying the geological evolution processes in the Korean peninsula, particularly within the context of radioactive waste disposal.

The seven-year research project entitled “Development of workflow for integrated 3D geological site descriptive modeling” is being carried out from 2023. This research is funded by Ministry of Trade, Industry, and Energy (MOTIE). Progress of the research is discussed here. The integrated 3D geological SDM (site descriptive model; GSDM hereafter) consists of three part; 1) three dimensional representation of geologic elements, 2) database for material properties and modeling results from SDMs of other disciplines (e.g., rock mechanics), and 3) a visualization tool for geology, material properties and modeling results. The GSDM is comparable to the GDSMs of SKB and POSIVA in its representation of geology by volume of geologic elements. However, our GSDM is different in that extra information of material properties and an extra tool for visualization is included in the GDSM. The rationale for incorporating material properties and a visualization tool into the GSDM is to expedite the development of the GSDM and SDMs of other disciplines by allowing single institution to integrate database and visualization with the GSDM. SKUA-GOCAD is used for representation of geologic surfaces for ductile and brittle shear zones, and also for surfaces for delineation of volumes of rock units. We have adopted SKUAGOCAD because the program offers powerful functions of interpolation including borehole data and geophysical prospecting. So far, we have tested the program for five different geologies, including sedimentary, high-grade metamorphic, and intrusive igneous geology. The test results are promising. Incorporation of data and modeling results for the SDMs of other disciplines is at conceptual stage. The working conceptual model involves the following steps, 1) to provide the modeler of other disciplines with surface information representing geologic elements, 2) the modeler returns not only material properties but the results of numerical analysis, and 3) incorporation of material properties and modeling results into database. Since the numerical codes in other disciplines adopt different types of formats for 3D geology, we plan to adopt the widely used FEM format prepared by Gmsh. The visualization tool will also adopt Gmsh for graphical representation of 3D geology as well as database for material properties and modeling results. When the working model of GSDM becomes available, rapid and significant progress is expected in the SDMs of other disciplines and related areas, for example, geotechnical investigation for deep geological repository.

The effectiveness of a crystalline natural barrier in providing sealing capabilities is based on the behavior of numerous fractures and their intersections within the rock mass. It is important to evaluate the evolving characteristics of fractured rock, as the hydro-mechanical coupled processes occurring through these fractures play a dominant role. KAERI is actively developing a true tri-axial compression test system and concurrently conducting hydro-mechanical experiments using replicated fractured rock samples. This research is focused on a comprehensive examination of coupled processes within fractures, with a particular emphasis on the development of true tri-axial testing equipment. The designed test system has the capability to account for three-dimensional stress conditions, including vertical and both maximum and minimum horizontal principal stresses, realizing the disposal conditions at specific underground depths. Notably, the KAERI-designed test system employs the mixed true tri-axial concept, also known as the Mogi-type, which allows for fluid flow into fractures under tri-axial compression conditions. This system utilizes a hydraulic chamber to maintain constant stress in one direction through the application of oil pressure, while the other two directional stresses are applied using rigid platens with varying magnitudes. Once these mechanical stress conditions are established, control over fluid flow is achieved through the rigid platens in contact with the specimen section. This pioneering approach effectively replicates in-situ mechanical conditions while concurrently observing the internal fluid flow patterns within fractures, thereby enhancing our capacity to study these coupled phenomena. As future research, numerical modeling efforts will be proceeding with experimental data-driven approaches to simulate the coupled behavior within the fractures. In these numerical studies, two distinct fracture geometry domains will be generated, one employing simplified rough-walled fractures and the other utilizing mismatched rough-walled fractures. These investigations mark the preliminary steps in the process of selecting and validating an appropriate numerical model for understanding the hydro-mechanical evolution within fractures.

It is crucial to understand the hydro-mechanical behavior of rock mass to assess the performance of natural barriers. As rock fractures serve as both mechanically weak planes and prominent pathways for hydraulic flow, they significantly influence the hydro-mechanical behavior of the rock mass. Hence, understanding the characteristics of rock fractures is necessary to analyze the long-term behavior of natural barriers. In particular, fracture apertures are crucial parameters directly associated with groundwater flow and consequently hold significant importance in determining the hydro-mechanical behavior of natural barriers. Fracture apertures are defined as mechanical and hydraulic apertures, and various studies have been conducted to measure and analyze them. However, direct measurement of mechanical aperture according to changes in normal stress is known to be a challenging task. For this reason, there has been a scarcity of direct comparative findings between mechanical and hydraulic apertures under various normal stress conditions. This study aims to analyze the characteristics of the mechanical and hydraulic apertures according to changes in normal stress based on experimental results. A digital analysis technique using a pressure film image was applied to analyze the mechanical aperture characteristics of the fracture. This technique can be applied by performing a pressure film compression test and a normal stiffness test on a fracture specimen, and has the advantage of being able to derive mechanical apertures under various normal stress conditions. The hydraulic aperture characteristics of the fracture were analyzed based on Cubic law after measuring the flow rate by performing a constant pressure injection test under triaxial compression conditions. By applying various confining pressures, it was possible to examine the hydraulic apertures according to changes in normal stress conditions. Through the experimental results, the relationship between the mechanical and hydraulic apertures of the fracture was summarized under various normal stress conditions. In addition, the experimental results were used to examine the applicability of various empirical equations for mechanical and hydraulic apertures proposed in previous studies. The characteristics of the fracture aperture resulting from this study are significant because they are required in the hydro-mechanical model of natural barriers. Future studies will entail further experiments, with the objective of establishing novel relationships based on the accumulation of experimental data.

The nuclide management process for reducing the environmental burden being developed by the Korea Atomic Energy Research Institute is performed in molten salts, resulting in contaminated salt wastes containing fission products such as Cs, Sr, Ba, and rare-earth nuclides. In addition, the spent fuel of a molten salt reactor (MSR) contains a variety of fission products, and a purification process may be required for the reuse of the salt and the separation and disposal of the fission products in the spent nuclear fuel. The melt-crystallization method is a technique used for the purification and separation of chemicals or metals based on the different melting points of the different substances. In a recent study, our group developed a reactive-crystallization method using Li2CO3 precipitation agent to precipitate metal corrosion from the reactor through a chlorination reaction by HCl and Cl2, which may occur in chloride molten salt, and successfully precipitated the metal precipitate and purified and recovered LiCl salt. In this study, reactive-crystallization method has been established for removing fission products and corrosive materials. Using the reactive crystallization method, white LiCl-KCl salt that was not discolored by metal corrosion was recovered through the crystallization plates, and fission products and metal elements were shown to be suppressed to several ppm in the purified salt. Consequently, high-purity salts were recovered with high nuclide and corrosive separation efficiencies. The reactive crystallization procedure can also be applied to other salt waste systems, such as MSR nuclear fuel treatment and molten salt chemistry for the elimination of corrosive substances.

The solid-state chemistry of uranium is essential to the nuclear fuel cycle. Uranyl nitrate is a key compound that is produced at various stages of the nuclear fuel cycle, both in front-end and backend cycles. It is typically formed by dissolving spent nuclear fuel in nitric acid or through a wet conversion process for the preparation of UF6. Additionally, uranium oxides are a primary consideration in the nuclear fuel cycle because they are the most commonly used nuclear fuel in commercial nuclear reactors. Therefore, it is crucial to understand the oxidation and thermal behavior of uranium oxides and uranyl nitrates. Under the ‘2023 Nuclear Global Researcher Training Program for the Back-end Nuclear Fuel Cycle,’ supported by KONICOF, several experiments were conducted at IMRAM (Institute of Multidisciplinary Research for Advanced Materials) at Tohoku University. First, the recovery ratio of uranium was analyzed during the synthesis of uranyl nitrate by dissolving the actual radioisotope, U3O8, in a nitric acid solution. Second, thermogravimetric-differential thermal analysis (TG-DTA) of uranyl nitrate (UO2(NO3)2) and hyper-stoichiometric uranium dioxide (UO2+X) was performed. The enthalpy change was discussed to confirm the mechanism of thermal decomposition of uranyl nitrate under heating conditions and to determine the chemical hydrate form of uranyl nitrate. In the case of UO2+X, the value of ‘x’ was determined through the calculation of weight change data, and the initial form was verified using the phase diagram for the U-O system. Finally, the formation of a few UO2+X compounds was observed with heat treatment of uranyl nitrate and uranium dioxide at different temperature intervals (450°C-600°C). As a result of these studies, a deeper understanding of the thermal and chemical behavior of uranium compounds was achieved. This knowledge is vital for improving the efficiency and safety of nuclear fuel cycle processes and contributes to advancements in nuclear science and technology.

In nuclear fuel development research, consideration of the back-end cycle is essential. In particular, a review of an in-reactor performance of nuclear fuel related to the various degradation phenomena that can occur during spent fuel dry storage is an important area. The important factors affecting the degradation of zirconium-based cladding during dry storage are the cladding’s hydrogen concentration and rod internal pressure after irradiation. In this study, a preliminary analysis of the in-reactor behavior of the HANA cladding, which has been developed and is currently undergoing licensing review, was performed, and based on this result, a comparative analysis between nuclear fuel with HANA cladding and current commercial fuel under storage conditions was performed. The results show that the rod internal pressure of nuclear fuel with HANA cladding is not significantly different from that of commercial cladding, and the hydrogen concentration in the cladding tends to reduce due to the increased corrosion resistance, so fuel integrity in a dry storage conditions is not expected to be a major problem. Although the lack of cladding creep data under dry storage conditions, the results from the Halden research reactor test comparing in-reactor creep behavior with Zircaloy-4 showed that there is sufficient margin for degradation due to creep during storage.

Once discharged, spent nuclear fuel undergoes an initial cooling process within deactivation pools situated at the reactor site. This cooling step is crucial for reducing the fuel’s temperature. Once the heat has sufficiently diminished, two viable options emerge: reprocessing or interim storage. A method known as PUREX, for aqueous nuclear reprocessing, involves a chemical procedure aimed at separating uranium and plutonium from the spent nuclear fuel. This separation not only minimizes waste volume but also facilitates the reuse of the extracted materials as fuel for nuclear reactors. The transformation of uranium oxides through dissolution in nitric acid followed by drying results in uranium taking the form of UO2(NO3)2 + 6H2O, which can then be converted into various solid-state configurations through different heat treatments. This study specifically focuses on investigating the phase transitions of artificially synthesized UO2(NO3)2 + 6H2O subjected to heat treatment at various temperatures (450, 500, 550, 600°C) using X-ray Diffraction (XRD) analysis. Heat treatments were also conducted on UO2 to analyze its phase transformations. Additionally, the study utilized XRD analysis on an unidentified oxidized uranium oxide, UO2+X, and employed lattice parameters and Bragg’s law to ascertain the oxidation state of the unknown sample. To synthesize UO2(NO3)2 + 6H2O, U3O8 powder is first dissolved in a 20% HNO3 solution. The solid UO2(NO3)2 + 6H2O is obtained after drying on a hotplate and is subsequently subjected to heat treatment at temperatures of 450, 500, 550, and 600°C. As the heat treatment temperature increases, the color of the samples transitions from orange to dark green, indicating the formation of different phases at different temperatures. XRD analysis confirms that uranyl nitrate, when heattreated at 500 and 550°C, oxidizes to UO3, while the sample subjected to 600°C heat treatment transforms into U3O8 due to the higher temperature. All samples exhibit sharp crystal peaks in their XRD spectra, except for the one heat-treated at 450°C. In the second experiment, the XRD spectra of the heat-treated UO2 consistently indicate the presence of U3O8 rather than UO3, regardless of the temperature. Under an oxidizing atmosphere within a temperature range of 300 to 700°C, UO2 can be oxidized to form U3O8. In the final experiment, the oxidation state of the unknown UO2+X was determined using Bragg’s law and lattice parameters, revealing that it was a material in which UO2 had been oxidized, resulting in an oxidation state of UO2.24.

The developmental biology and morphological characteristics of the immature stages of Hypolimnas bolina were studied in the laboratory using host plant, Ipomoea batatas (L.) Lam. This species has six larval stadia, with an egg period of 2.4 ± 0.54 days, a larval period of 16.8 ± 2.3 days, and a pupal period of 10 ± 1 days. It takes approximately 30 days to mature from egg to adult. Illustrations and descriptions of the various immature stages and their behaviors are provided.

농가 현장에서는 미소 해충의 약제저항성 발달로 인하여 방제에 어려움을 겪고 있다. 본 연구는 약제저항성 수준을 효율적으로 진단하고, 화학적 방제 의사결정을 지원할 수 있는 약제저항성 관리 플랫폼을 구축하고자 수행하였다. 플랫폼은 크게 ⅰ) 농가 맞춤형 약제 추천, ⅱ) 지역별 약제저항성 지도로 구성되어 있다. 이용자는 잔류접촉법 기반의 생물검정법 RCVpW(Residual Contact Vial plus Water) 결과를 현장에서 입력하면 PLS에 부합 한 약제 정보를 제공받을 수 있다. 또한 생물검정 결과는 DB화를 통해 지역별 약제 저항성 정보 지도를 보여줌으 로써 해충별, 작물별, 연도별 저항성 패턴 결과를 확인할 수 있다. 본 플랫폼을 활용한다면 약제의 오남용을 줄임 은 물론 해충의 약제저항성 발달 지연 및 약제저항성 발달 양상을 확인할 수 있어 향후 방제전략 수립에 활용할 수 있을 것으로 생각된다.