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pp.50-55
반수체 육성은 육종연한을 단축시키기 위해 유용한 도구이다. 본 연구는 밀 품종 조기 육성을 위한 반수체 육종 시스템을 개발하기 위해 반수체 육성시 식물생장조절제 처리가 배 배양에 미치는 영향에 대해서 조사한 것이다. 밀 이삭의 제웅 작업을 수행하고 옥수수와 원연교잡한 후 종자결실은 2,4-D 및 2,4,5-T 150 mg/L, dicamba 50 mg/L 처리에서 가장 좋았으며, 배형성은 2,4-D 및 2,4,5-T 100 mg/L, dicamba 50
The introduction of doubled haploid (DH) approach into breeding programs has reduced the times and population sizes required for the production of pure lines. We carried out the experiment for development on effective method of producing haploid in wheat. Emasculated spikelets of wheat were pollinated with maize pollen and cultured in the solution containing 40 g/L sucrose and 2,4-D, NAA, 2,4,5-T and dicamba 24 h after pollination, and then incubated until embryo rescue. Fourteen to sixteen days after pollination, the embryos are excised and cultured in half-strength MS basal medium supplemented with 20 g/L sucrose and 1 mg/L NAA. The type of plant growth regulators was found to be most significant in production of haploid plants. The application of synthetic auxins to pollinated florets, stimulates haploid embryo development to a stage where the embryos can be rescued onto nutrient media. The percentage of seeded florets was significantly affected by 100 mg/L 2,4-D, 150 mg/L 2,4,5-T and 50 mg/L dicamba. The percentage of embryos formed was significantly increased by treatment with 2,4-D and 2,4,5-T at 100 mg/L, and dicamba at 50 mg/L, but the treatments with 150 mg/L 2,4-D inhibited embryo development and plant regeneration. The optimum application time of plant growth regulators was 24 hrs after pollination.
