dlm mutants controlling disease lesion mimic leaf trait may be useful in basic research of disease hypersensitive response and programmed cell death in soybean. The study on genetic relationship between dlm trait and other morphological C trait, position of dlm allele on classical linkage group, and a molecular marker linked to dlm allele was little reported. Two populations [T173 (ffDlmDlm) x T363 (FFdlmdlm), T363 (dlmdlmY9Y9) x T135 (DlmDlmy9y9)] were made to find independent assortment or linkage between dlm locus and f locus or between dlm locus and y9 locus. The segregation ratios of 3 : 1 were observed in the F2 population and the Chi-square values suggested that the disease lesion mimic leaf, fasciation stem, and chlorophyll-deficient leaf traits were controlled by a single recessive gene. Segregation ratios of 78 Dlm_F_: 19 Dlm_ff: 17 dlmdlmF_ : 3 dlmdlmff based on F2 phenotype showed that dlm allele was inherited independently with the f allele controlling fasciation stem trait in soybean. Also, segregation ratios of from 149 Dlm_Y9_: 41 Dlm_y9y9: 38 dlmdlmY9_ : 5 dlmdlmy9y9 based on F2 phenotype confirmed that dlm allele was inherited independently with the y9 allele controlling chlorophyll- deficient leaf trait in soybean. From these results, dlm allele would not be located on linkage group 11 (molecular linkage group: D1b+W) and linkage group 14 (molecular linkage group: E) in soybean. This results will helpful to attempt to position the Dlm locus on the soybean molecular linkage map.

dlm allele controlling disease lesion mimic trait is useful in basic research aimed at better understanding disease hypersensitive response and programmed cell death in soybean. Inheritance between dlm trait and any morphological trait, position of dlm allele on classical linkage group, molecular marker linked to dlm allele were not reported. Two populations [T255 (lf2lf2DlmDlm) x T363 (Lf2Lf2dlmdlm), T363 (dlmdlmp1p1) x T43 (DlmDlmP1P1)] were made to find independent assortment or linkage between dlm locus and lf2 or between dlm locus and P1 locus. The segregation ratios of 3 : 1 were observed in the F2 population and the Chi-square values strongly suggested that the disease lesion mimic and seven-leaflet trait was controlled by a single recessive gene. Glabrousness trait was controlled by a single dominant gene. Segregation ratios of 48 Lf2_Dlm_: 30 Lf2_dlmdlm: 21 lf2f2Dlm_ : 8 lf2lf2dlmdlm based on F2 phenotype showed that dlm allele was inherited independently with the lf2 allele controlling seven-leaflet trait in soybean. However, more F2 plants will be needed to confirm this result. Also, segregation ratios of 137 P1_Dlm_: 46 P1_dlmdlm: 49 p1p1Dlm_ : 16 p1p1dlmdlm from F2 phenotype confirmed strongly that dlm allele was inherited independently with the P1 allele controlling glabrous trait in soybean. This results indicate that dlm allele will not located in soybean classical linkage group 2 (molecular linkage group K) and soybean classical linkage group 16. This observation will helpful to attempt to position the Dlm locus on the soybean molecular linkage map.

Leaflet number of soybean controlled by Lf2 locus is the important trait in photosynthesis and plant type. The objective of this research was to identity molecular markers linked to the lf2 locus. A total of 115F2 plants were derived from a cross between normal three-leaflet type Sinpaldalkong (Lf2Lf2) and seven-leaflet mutant type T255 (lf2lf2). All leaflet counts of parents and F2 individual plants were made in the field on fully expanded leaves on the main stem when terminal growth of the main stem had ceased. One-thousand 10-mer oligonucleotide RAPD primers and 664 SSR primers were used. The segregation ratios of 3 : 1 were observed in the F2 population and the Chi-square values strongly suggested that the seven-leaflet was controlled by a single recessive gene. A genetic map was constructed from the 15 segregating markers (9 RAPDs, 5 SSRs, 1 lf2 locus). OPAD03 and OPAI13 RAPD markers were linked to the lf2 locus that controlled seven-leaflet type at a distance of 20.5 and 23.5 cM, respectively. Molecular markers identified in this study linked with lf2 locus will be helpful to locate lf2 locus on the public soybean molecular linkage map and would be useful for tagging the lf2 locus that controls seven-leaflet trait.

Leaf chlorophyll-deficient mutants controlled by y9 locus have been observed frequently and are useful in genetic stud-y9 locus. A mapping population con-sisting of 94 F2 progeny was derived from a cross between normal green leaf Clark (Y9 ) and chlorophy