의학적 중요성에도 불구하고 다년생 약용식물의 하나인 황기(Astragalus membranaceus)와 관련된 분자육종 연구의 전무한 편이다. 본 연구에서는 황기의 유묘를 대상으로 agroinfiltration법을 이용한 in planta 형질전환을 시도하였다. 형광 및 염색법에 의한 GUS발현 분석에서 황기는 Agrobacterium 감염이 쉽지 않은 것으로 나타났다. 그러나 발아 후 2-3일이 결과한 유묘에 대하여 NaOH나 과산화수소를 적절히 처리

In spite of medicinal importance, Astragalus membranaceus, a perennial herbal plant, has been little studied for its molecular breeding. In this study, in planta genetic transformation using its seedling was performed by means of agroinfiltration. In the analysis of fluorometric and histochemical GUS assay, it was determined that A. membranaceus seemed not very susceptible to Agrobacterium infection. However, a successful transformation could be achieved when 2 to 3 days-old seedlings, prior to agroinfiltration, were moderately wounded by NaOH and hydrogen peroxide treatment. The results can suggest an efficient way for developing a more desirable A. membranaceus cultivars in light of cultivation as well as functional properties.

• 신동일(대구가톨릭대학교 생명공학과) | Dong Il Shin
• 박희성(대구가톨릭대학교 생명공학과) | Hee Sung Park