蓮(Nelumbo mucifera Gaertn)은 그 아름다운 꽃으로 유명하지만 또한 연 식물체의 거의 모든 부위는 식용 및 약용으로 쓰일 수 있다. 본 연구에서는 蓮根을 대상으로 Agrobacterium 세포감염 방법으로 유전적인 형질전환을 시도하였다. 즉, 蓮根을 1 mm 정도의 두께로 얇게 썰어서 절편으로 준비한 후 이에 대해 agroinfiltration 및 동시배양(3일, 22℃, 암 조건)을 수행하였다. 이러한 일반적인 agroinfiltra

Lotus (Nelumbo nucifera Gaertn) is pretty well known for the beauty of its water flowers. And, it is consumed as food as well as medicine. In this study, lotus rhizome was genetically transformed by means of Agrobacterium cell infection. Lotus rhizome was thinly sliced to about 1 mm in depth prior to agroinfiltration and continuing cocultivation for 3 days at 22℃ in the dark. By conventional agroinfiltration process no sign of GUS transformation was detected in the analysis of fluorometric and histochemical assay. Treatment to the lotus slices of chemicals such as NaOH, hydrogen peroxide and sodium dodecyl sulfate greatly enhanced transformation rate. Therefore, for the lotus transformation, cell wall architecture can be considered as a major barrier against Agrobacterium infection and genetic transformation.

• 박희성(대구가톨릭대학교 생명공학과) | Hee Sung Park
• 신동일(대구가톨릭대학교 생명공학과) | Dong Il Shin