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Antioxidant activities and anti-inflammatory effects of fresh and air-dried Abeliophyllum distichum Nakai leaves KCI 등재 SCOPUS
건조방법에 따른 미선나무 잎의 항산화 및 항염증 효과
- 언어KOR
- URLhttps://db.koreascholar.com/Article/Detail/344192
pp.27-35
In this study, we evaluated the antioxidant activity and anti-inflammatory effects of Abeliophyllum distichum (A. distichum) leaves that were prepared via air-drying. Fresh and air-dried A. distichum leaves were examined via 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical, and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging assay and measurements of the reducing power. The suppression effects on inflammation of the leaves were analyzed by a western blot and RT-PCR on LPS-induced RAW 264.7 cells. As a result, the antioxidant activity of the fresh leaves was found to be more effective than that of the air-dried leaves. Also, the fresh leaves were more effective in suppressing the protein and mRNA levels of iNOS and COX-2 than the air-dried leaves, thereby indicating the better anti-inflammatory effects. In addition, the contents of phenolic compounds and acteoside were analyzed by high-performance liquid chromatography (HPLC). The results showed that the acteoside content decreased with the use of the air-drying method, while there was no change in the content of phenolic compounds. Therefore, this study indicated that fresh A. distichum leaves potential antioxidant and suppression activities of various factors that are involved in the production of NO, which were found to be better than those of air-dried A. distichum leaves. These biological activities were also found to be independent of the content of phonolic compounds and were assumed to be directly or indirectly related to the content of acteoside.
재료 및 방법
실험재료
가공 및 저장 조건
DPPH 라디칼 소거활성
환원력
총 페놀 함량 분석
Acteoside 함량 분석
세포 배양
세포 생존율
NO 생성량 측정
Western blot
Total RNA 추출 및 cDNA 합성
RT-PCR
통계학적 분석
결과 및 고찰
항산화 활성
페놀류 화합물 함량 분석
Acteoside 함량 분석
세포 생존율
항염증 활성
요 약
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