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Anti-inflammation effect of blueberry (Vaccinium ashei) leaf extract on RAW 264.7 macrophages stimulated by lipopolysaccharide KCI 등재 SCOPUS
Lipopolysaccharide에 의해 활성화된 RAW 264.7대식세포에서 블루베리 잎(Vaccinium ashei) 추출물의 항염증 효과
- 언어KOR
- URLhttps://db.koreascholar.com/Article/Detail/344201
pp.107-116
The aim of this study is to investigate the antioxidant and intracellular anti-inflammatory efficacy of blueberry leaf extracted with hot water (BLW), 70% ethanol (BLE), and 70% acetone (BLA) in RAW 264.7 macrophages. In order to evaluate the anti-inflammatory effect of blueberry leaf extracts, RAW 264.7 macrophages were stimulated with lipopolysaccharide (LPS) to induce the production of inflammation-related factors, which were measure by Western blotting and real-time PCR methods. i-NOS, COX-2 protein, and mRNA expression showed concentration-dependent decrease. The decreases in the mRNA expression levels of interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2) were concentration-dependent. Further, the antioxidant effects of blueberry leaf on total polyphenol contents, electron donating ability and ABTS+ radical scavenging activity were evaluated. The total polyphenol contents of BLW, BLE, and BLA were 217.04±2.98, 156.72±3.90, and 182.88±3.02 mg TAE/g, respectively, while the electron donating abilities at 1,000 μg/mL of BLW, BLE, and BLA were 81.7, 79.6, and 79.3%, respectively. The ABTS+ radical scavenging activity was fond to be concentration dependent. The nitric oxide (NO) production inhibition activities at 50 μg/mL of BLW, BLE, and BLA were 35.1, 42.4 and 42.7%, respectively. In conclusion, the antioxidant and anti-inflammatory test results indicate that blueberry leaf extracts (BLW, BLE, and BLA) can be used as potential anti-inflammatory agents.
재료 및 방법
재료 및 시료추출
항산화능 분석 시약
항염증 측정 시약
세포 독성 측정에 사용된 세포주 및 시약
실험에 사용된 기기
총 폴리페놀 화합물 함량 측정
전자공여능 확인
ABTS cation radical scavenging activity 확인
Xanthine oxidase inhibition activity 확인
세포 배양
MTT assay에 의한 세포독성 확인
Nitric oxide 생성량 측정
Western blot
PGE2분비량 측정
cDNA 합성
Real-time PCR
통계처리
결과 및 고찰
총 폴리페놀 화합물 함량
Blueberry(Vaccinium ashei) leaf의 전자공여능
ABTS+ radical scavenging activity assay
Superoxide anion radical 저해활성
Macrophage cell(RAW 264.7)의 세포 독성 확인
Nitric oxide 저해활성
Western blot을 통한 i-NOS 및 COX-2 protein 발현 억제율
PGE2 저해활성
Real-time PCR을 통한 다양한 mRNA 발현 억제 효과
고 찰
요 약
감사의 글
References
