In this study, we investigated the response of freshwater oligochaete, Tubifex tubifex, to the water temperature changes and the differences of substrate composition in a laboratory condition. The changes of body shape were observed in a test cage according to the water temperature change ranging from 10℃ to 30℃ with 2℃ interval every 10 minutes. The substrate preference was observed with four different substrate composition from silt-clay to coarse sand. Our results displayed that T. tubifex preferred substrates with the smallest particle size (<0.063 mm). The water temperature influenced on the activity and body shape of T. tubifex, showing low activity with the coiled and constricted body shapes at lower temperature and high activity with relaxed linear body shapes at higher temperature.

Abstract
 서 론
 재료 및 방 법
  1. 실험동물
  2. 수온 증가에 따른 행동 반응
  3. 참실지렁이의 하상선호도 실험
  4. 통계 분석
 결 과
  1. 수온 증가에 따른 행동 반응
  2. 하상 선호성
 토의 및 결 론
 적 요
 References

• 강혜진(경희대학교 생물학과) | Kang, Hyejin (Department of Biology, Kyung Hee University)
• 배미정(국립낙동강생물자원관 담수생물다양성연구실) | Mi-Jung Bae (Freshwater Biodiversity Research Division, Nakdonggang National Institute of Biological Resources)
• 박영석(경희대학교 나노의약생명과학과) Corresponding Author