NSF developed NSF p477, which covers POU active media systems. Exact types and quantities of cyanotoxins vary. In 2015 USEPA set recommended health advisories for microcystins in drinking water at or below 0.3 μg/L for children less than 6 years old, and at or below 1.6 μg/L for older children and adults. NSF developed and characterized a natural source for microcystin compounds to be used as a contaminant challenge for the POU active media systems being tested. Analytical methodologies were refined to reliably identify and quantify microcystin at levels in the sub part per billion range. Laboratory column studies using various activated carbons were conducted to determine the relative retention of various microcystin congeners, to assess the appropriateness of various congeners for testing purposes.