논문 상세보기
pp.143-149
건강기능식품 등 중 수용성 비타민 B군 (비타민 B1, 비타민 B2, 나이아신, 비타민 B6)의 동시분석 방법을 확립하기 위하여 본 연구를 실시하였다. HPLC에 의한 수용성 비타민 B군의 분석 방법은 기기분석조건을 확립하고, 국내외 시험법 및 논문을 참고하여 시험 데이터를 분석하였다. 수용성 비타민 B군의 HPLC 분석은 270 nm에서 Capcell Pak C18 UG 120 컬럼을 사용하여 진행되었다. 분석법 검증은 수용성 비타민 B군의 직선성, 정확성, 정량한계, 정밀성에 대해 수행되었다. 직선성은 비타민 B1, 비타민 B2,나이아신(nicotinic acid, nicotinamide), 비타민 B6 모두0.1~2 μm/mL의 농도범위에서 R2= 0.999 이상의 우수한 직선성을 확인할 수 있었다. 회수율을 검토한 결과, 비타민 B1 100~103%, 비타민 B2 104~112%, nicotinic acid 82~85%, nicotinamide 121~124%, 비타민 B6 95~104%의 양호한 회수율을 확인할 수 있었다. LOQ는 비타민 B1 0.04μm/mL, 비타민 B2 0.05 μm/mL, nicotinic acid 0.15 μm/ mL, nicotinamide 0.08 μm/mL, 비타민 B6 0.63 μm/mL 이었다. 정밀성을 검토한 결과, B1 0.4%, 비타민 B2 0.4%, nicotinic acid 0.5%, nicotinamide 0.7%, 비타민 B6 0.4% 로 1% 이내로 양호한 재현성을 확인할 수 있었다. 또한 동시 분석법의 정확성을 검증하기 위하여 어린이 기호식품 및 건강기능식품의 표시함량 모니터링을 수행한 결과, 모두 표시기준에 적합함을 확인할 수 있었다.
This study was conducted to simultaneous analysis methods for water soluble vitamins B group (vitamin B1, vitamin B2, nicotinic acid, nicotinamide, vitamin B6) which is used as health functional foods etc. Analytical methods of water-soluble vitamins B group by HPLC were established through instrumental analytical conditions, and the examination of data such as domestic and foreign reliable methods, and papers of journal. HPLC method analyzing water soluble vitamins B group was established using Capcell Pak C18 UG 120 column in 270 nm through test of columns. The validation has been performed on the method to determine linearity, accuracy, limits of quantification (LOQ) and repeatability for water soluble vitamins B group. An excellent linearity (r2 = 0.999) was observed for vitamin B1, vitamin B2, nicotinic acid, nicotinamide, vitamin B6 in the concentration range (0.1~2 μg/mL). Observed recovery of vitamin B1 was found to be between 100 and 103%, vitamin B2 was found to be between 104 and 112%, nicotinic acid was found to be between 82 and 85%, nicotinamide was found to be between 121 and 124% and vitamin B6 was found to be between 95 and 104%. LOQ of vitamin B1 was found to be 0.04 μg/mL, vitamin B2 was found to be 0.05 μg/mL, nicotinic acid was found to be 0.15 μg/mL, nicotinamide was found to be 0.08 μg/mL and vitamin B6 was found to be 0.63 μg/mL. Repeatability precision for vitamin B1 was found to be 0.4%, vitamin B2 was found to be 0.4%, nicotinic acid was found to be 0.5%, nicotinamide was found to be 0.7% and vitamin B6 was found to be 0.4% relative standard deviation (RSD). Also, verify the accuracy of the simultaneous analysis methods, we monitored the labeled contents of the health functional foods and children's preferred foods.
실험 재료
표준품 및 시약
표준용액 및 시액의 조제
시험용액의 조제
HPLC 기기 및 분석조건
비타민 대상 시료의 함량 확인
Results and Discussion
크로마토그램
분석법 검증
시료의 분석
국문요약
Acknowledgement
References
