Allergic diseases have rapidly increased in recent years. Mast cells play a critical role in IgE-mediated allergy responses and, therefore, closely associated with rhinitis, asthma, and atopic dermatitis. We studied antiallergic effect of Penthorum chinense extract (PCE) in vitro and in vivo. PCE inhibited the degranulation of mast cells by antigen stimulation and its effect was dose-dependent and reversible in mast cells. PCE also suppressed the production of inflammatory cytokines such as TNF-α and IL-4 by antigen in mast cells. Mechanistically, PCE inhibited the activation of Syk/LAT pathway which is a key signaling pathway for the activation of mast cells by antigen. Notably, PCE suppressed IgE-mediated allergic responses by antigen in mice. These results strongly suggest that PCE is a potential candidate for anti-allergic treatment.

ABSTRACT
 Materials and Methods
  재료
  실험동물
  낙지다리의 메탄올 추출물(PCE) 제조
  비만세포(BMMC, RBL-2H3)의 분리 및 배양
  비만세포의 탈과립(β-hexosaminidase) 측정
  Cell viability
  Immunoblotting 분석
  국소적 수동 피부 알레르기 마우스 동물 모델
  통계처리
 Results
  비만세포(RBL-2H3 세포)의 탈과립(β-hexosaminidase) 억제효과
  PCE의 비만세포 탈과립 억제 효과의 가역성 실험
  PCE의 비만세포에서 염증성 사이토카인 분비 억제 효과
  PCE의 세포내 칼슘 농도 증가에 따른 탈과립의 억제 효과
  PCE의 비만세포에서의 Syk 및 LAT의 인산화 억제 효과
  PCE의 국소피부자극 알레르기 반응의 억제 효과
 Discussion
 References

• 조소영(덕성여자대학교 약학대학) | So young Jo
• 김영미(덕성여자대학교 약학대학) | Young Mi Kim Correspondence to