Background: The extract of Abies holophylla is used as an ingredient in cosmetics. This study assessed the antioxidant and antibacterial activities of the material remaining after the extract is used.
Methods and Results: The 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azinobis (3-ethyl benzothiazoline)-6-sulfonic acid (ABTS) radical scavenging abilities were assessed to determined the free radical scavenging activity. The total phenol and flavonoid contents were determined to measure the antioxidant activity. The DPPH and ABTS radical scavenging activities of the resudual extract were higher (95.61 - 99.42% and 74.26 - 77.98% in water extract respectively) than those of the positive control. In 50% EtOH extract, the total phenol content was 389.84 ㎎·GAE/㎖, and the total flavonoid was 0.15 ㎎·QE/㎖. The minimum inhibition concentration degree for antibacterial activity against Staphylococcus aureus was < 8 to < 125 ㎍/㎖ compared to that of the positive control in all extracts. The clear zone against S. aureus was found to be 12.2 ± 3.8 ㎜.
Conclusions: The A. holophylla byproducts were found to have antioxidant and antibacterial activities. Therefore, the materials remaining after the A. holophylla extract is used in cosmetics has potential functional uses. Key Words: Abies holophylla, Staphylococcus aureus, Antioxidant Activity, Antibacterial Activity

ABSTRACT
 서 언
 재료 및 방법
  1. 식물 및 공시재료
  2. 식물 추출물 준비
  3. DPPH를 이용한 항산화 활성 측정
  4. ABTS를 이용한 항산화 활성 측정
  5. 총 페놀 함량
  6. 총 플라보노이드 함량
  7. 미생물 배양
  8. 항미생물 활성 측정
  9. Disc diffusion법을 이용한 항균 활성 분석
  10. 통계처리
 결과 및 고찰
  1. DPPH와 ABTS를 이용한 항산화능 분석
  2. 총 페놀성 화합물과 총 플라보노이드 함량 비교 분석
  3. 박테리아에 대한 항미생물 활성 분석
 REFERENCES

• 성은수(수원여자대학교 약용식물과) | Eun Soo Seong (Department of Medicinal Plant, Suwon Women’s University) Corresponding author
• 김수경(수원여자대학교 약용식물과) | Soo Kyung Kim (Department of Medicinal Plant, Suwon Women’s University)
• 이진원(수원여자대학교 약용식물과) | Jin Won Lee (Department of Medicinal Plant, Suwon Women’s University)
• 최승혁(강원대학교 생물자원과학과) | Seung Hyuk Choi (Department of Bio-Resource Sciences, Kangwon National University)
• 유지혜(강원대학교 한방바이오연구소) | Ji Hye Yoo (Bioherb Research Institute, Kangwon National University)
• 임정대(강원대학교 생약자원개발학과) | Jung Dae Lim (Department of Herbal Medicine Resources, Kangwon National University)
• 라종국(강원대학교 평생학습중심학부) | Jong Kuk Na (Department of Controlled Agriculture, Kangwon National University)
• 유창연(강원대학교 생물자원과학과) | Chang Yeon Yu (Department of Bio-Resource Sciences, Kangwon National University) Corresponding author