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        검색결과 85

        1.
        2023.06 KCI 등재 구독 인증기관 무료, 개인회원 유료
        본 연구는 초등학생의 학습·사회성 부진에 영향을 미치는 요인을 분석하여, 부모 관계에 따른 아동기 심리적 안전감의 중요성을 밝히기 위한 연구이다. I시 J구의 초등학교 학부모 대상 안전 인 식 조사에서 주관적 건강, 성격 특성, 대인 관계, 정서·행동 특성의 심리적 안전감과 학교폭력 피 해 및 부모 관계를 통해 학습·사회성 부진의 영향요인을 분석하였다. 자료의 분석은 t-검증과 ANOVA를 활용하여 일반적 특성에 따른 차이를. 다중회귀분석으로 학습·사회성 부진의 영향요인을, 교차분석과 로지스틱 회귀분석으로 학습·사회성 부진의 위험도를 분석하였다. 분석의 결과는 첫째, 여아의 과민·반항성이 높았고, 6학년 아동의 개방성이 낮게 나타났으며, 부모의 학력이 높을수록 주관적 건강과 학교폭력 피해 인식이 높고, 월 300만 원대의 소득수준에서 학습·사회성 부진이 높 다. 둘째, 학습·사회성 부진의 영향요인으로 긍정적 관계에서는 불안·우울과 학교폭력 피해 경험으 로, 부정적 관계에서는 집중력과 불안·우울로 나타났다. 셋째, 학습·사회성 부진 위험도에 유의한 영향을 미치는 요인은 사회적 주도성, 불안·우울과 집중력 부진, 소득수준, 타인 이해, 공동체 의 식으로 나타나고 있다. 아동의 학습·사회성 부진은 불안·우울, 집중력 부진과 높은 상관성이 있고, 부정적 부모 관계에서 높은 영향을 받는다. 따라서 아동의 학습·사회성 향상을 위해서는 긍정적 부 모 관계와 심리적 안전감의 확보가 중요하다.
        5,700원
        2.
        2022.10 KCI 등재 구독 인증기관 무료, 개인회원 유료
        본 연구는 청소년 정서􌝆행동 특성이 자살 위험에 미치는 영향을 파악하고 위험집단의 취 약성 파악에 목적이 있다. 종속변수는 자살 생각과 계획, 시도를 포함하여 자살 위험으로 설정하였고, 독립변수는 일반적 특성과 학교폭력의 위험요인, 정서.행동 요인, 성격 요인으 로 설정하였다. 첫째, T-검정과 ANOVA를 활용하여 일반적 특성에 따른 각 변수의 차이를 파악한 결과 자살 위험과 정서.행동 문제에서 여학생의 위험성이 높게 나타났다. 약물 오남 용과 자존감, 부모와의 관계에서는 남학생이 높았으며, 성적에 따른 자살 위험의 차이는 유 의하지 않았다. 둘째, 다중회귀분석을 활용한 자살 위험 영향요인 파악에서는 성적과 자존 감이 높고, 부모 관계가 긍정적일수록 자살 위험이 낮아졌다. 자살 위험을 높이는 요인으로 는 심리적 부담(.733), 기분 문제(.327), 약물 오남용(.174), 인터넷 의존(.146) 순으로 나 타났다. 셋째, 교차분석을 활용한 집단별 차이를 분석한 결과 자살 위험의 비중이 높은 집 단은 심리적 부담, 기분 문제, 불안 문제의 관리군과 자기통제 부진의 일반군, 약물 오남용 관리군, 인터넷 의존 일반으로 파악되었다. 넷째, 로지스틱 회귀분석을 활용한 자살 위험성 분석에서는 중학교 1학년, 고등학교 1학년의 위험성이 높았고, 기분 문제 관리군, 약물 오 남용 관리군, 불안 문제 관리군, 인터넷 의존 관리군, 낮은 자존감 집단과 낮은 공동체 의식 집단에서 자살의 위험성이 큰 것으로 파악되었다. 청소년 자살 위험은 개인의 성격, 정서.행 동 특성 및 사회환경적 요인이 상호작용하여 복합적으로 나타나므로, 위험군의 개별적 접근 보다는 지역사회 공동체에 의한 협력 프로그램의 개입이 필요하다.
        6,100원
        3.
        2021.01 KCI 등재 구독 인증기관 무료, 개인회원 유료
        본 연구는 노인들의 성 건강과 성 건강 행태에 관한 질적 연구이다. 성 건강을 정신적, 신체적, 감정적, 사회적 성 건강으로 분류하여 성 건강 인식을 파악하였다. 성 건강에 영향을 미치는 행태특성은 삶의 만족도, 성적친밀감, 소통유형, 결혼 만족도, 배우자 만족도로 나타났다. 분석의 결과 배우자와의 관계와 성적친밀감, 소통방식의 행태특성이 성 건강에 영향을 미치는 요인으로 파악되었다. 성공적 노화는 행복감에서 시작하므로 성 건강은 노후의 삶의 질을 결정하는 요인이라 할 수 있다. 본 연구에서 나타난 성 건강과 행태특성을 반영한 성 건강 교육에 관한 후속연구가 필요함을 제언한다.
        5,200원
        4.
        2019.06 KCI 등재 구독 인증기관 무료, 개인회원 유료
        최근 인터넷 문화의 발달과 디지털 게임이 급속도로 발전하고 있다. 그에 따라 플랫폼의 변화도 일어나고 있으며 디지털 게임의 장르 또한 많이 세분화되며 남녀노소 즐길 수 있는 문화가 되었다. 그 중에서도 다중 이용자 온라인 롤플레잉 게임(Massive Multi-player Online Role Playing Game, MMORPG)이 크게 성장하며 이용자의 수가 급증하였다. 이 다중 이용자 온라인 롤플레잉 게임(MMORPG)은 수많은 사람들이 가상공간에서 만나 함께 게임을 즐길 수 있는 장르로, 가상공간에서 소통 및 관계를 맺기에 최적이라는 특징을 갖고 있다. 그러나 과거엔 콘텐츠적 특성이 폭력적, 선정성이 강해 남성 이용자들의 전유물이라는 경향이 강했으나 최근엔 여성 이용자들이 꾸준히 증가하고 있다. 이에 관한 여성 이용자들의 MMORPG 게임 콘텐츠에 대한 선호양상을 연구하고자 한다.
        4,600원
        5.
        2015.09 KCI 등재 구독 인증기관 무료, 개인회원 유료
        1. 국내에서 수집한 수수의 성숙기 간장이 250 cm 이상되는자원은 5 종으로 전체 62 수집종의 8.1 %를 차지하였다. 2. 수형 특성 조사에서는 3개 분얼수를 가지는 자원은 전체수집종의 56.6%를 나타낸 것으로 보아, 평균 분얼수는 3개 인것으로 나타났다. 3. 잎의 특성조사에서 엽장이 90 cm 이상되는 자원은 14.5%로 분류되었다. 4. 분얼형에 있어서는 반밀수타원형이 전체 수수 수집종의32.3%를 차지하였다. 5. 전체 수집종 수장의 평균값은 29.2 cm로 나타났다.
        4,000원
        6.
        2007.09 KCI 등재 구독 인증기관 무료, 개인회원 유료
        본 연구의 목적은 치매노인의 보호를 위해 필요한 주요문제특성의 추출이다. 시설에 입소해 있는 치매노인 110명과 정상노인 30명을 대상으로 기본특성 조사, 인지기능 선별검사(Korean Mini-Mental State Examination: K-MMSE), 문제특성 설문조사를 실시하였다. 요인분석(factor analysis)을 이용하여 치매노인의 주요문제특성을 추출하고, 일원변량분석(one-way ANOVA)을 이용하여 성별과 치매 중증도에 따른 문제특성의 빈도차이를 검증하였다. 요인분석결과 총 20개의 주요문제특성이 추출되었다. 문제특성의 빈도는 성별에 따라 폭력적 언어 및 작화, 수집행동, 반복행동에서 유의한 차이가 있었다. 치매 중증도에 따라서는 성적 이상행동과 시청력 장애를 제외한 모든 특성에서 유의한 차이가 나타났다. 본 연구 결과는 치매노인 보호를 위한 유비쿼터스 기술의 활용에 기초자료를 제공할 수 있을 것이다.
        4,000원
        7.
        2007.03 KCI 등재 구독 인증기관 무료, 개인회원 유료
        치매노인의 주요 문제특성의 추출을 위해 시설에 입소해 있는 치매노인 110명을 대상으로 기본특성 조사, 인지기능 선별검사, 문제특성 설문조사를 실시하였다. 요인분석결과 총 20개의 주요 문제특성이 추출되었다. 본 연구에서는 치매노인 보호를 위해 주요 문제특성 20개의 위험순위를 결정하였다. 치매진단 및 치매환자를 접한 경험이 있는 정신보건 임상심리사 32명을 대상으로 설문조사를 실시하였다. 계층분석법을 이용하여 상대적 위험도를 분석하였고, 20개의 문제특성 전체에 대하여 위험도 서열을 정하도록 하여 위험순위를 결정하였다. 두 가지 분석 결과 모두 치매 노인의 일상생활 수행 시 기억력, 판단력, 지남력 장애와 같은 인지문제가 가장 위험한 것으로 나타났다.
        4,000원
        8.
        2006.03 KCI 등재 구독 인증기관 무료, 개인회원 유료
        본 연구의 목적은 21%농도의 산소와 비교하여 30%의 고농도 산소 흡입이 사이클 등속 운동 중에 심박동률(heart rate)과 혈중 산소 포화도(SPO2)에 미치는 영향을 분석하는 것이다. 21%와 30% 농도의 산소를 각각 8liter/min의 양으로 일정하게 공급할 수 있는 산소 공급 장치를 이용하였다. 신체 건강한 10명의 남자 대학생(25.2±2.2세)이 21%와 30%의 두 종류의 산소 농도를 대상으로 각각 두 번의 실험에 참여하였다. 실험은 안정(2분), 20±1km/h의 사이클 등속 운동(10분), 회복(6분)의 세 구간(총 18분)으로 구성되어 있고, 21%또는 30%의 산소는 등속 운동과 회복 구간에만 공급되었다. 21%에 비해 30%산소를 흡입했을 때 사이클 등속 운동과 회복 기간 중에 심박동률은 감소하였고, 혈중 산소 포화도는 .차이가 없었다. 21%와 30% 두 그룹 사이에 동일한 운동부하가 주어졌기 때문에 혈중 산소 포화도는 차이가 없는 것으로 판단되고, 30%의 고농도 산소 흡입으로 동일운동 부하에 필요한 산소 요구량이 원활히 공급되고 있기 때문에 심박동률은 감소한 것으로 판단된다.
        4,000원
        9.
        2018.10 서비스 종료(열람 제한)
        Background : Miscanthus sinensis is a C4 crop with high photosynthetic efficiency and is known as a representative cellulose-based bio-energy crop originating in East Asia such as Korea, Japan, and China. It also has a high resistance to environmental conditions such as low temperature and drying and pests. However, many studies on the antioxidant activity of the M. sinensis have not been conducted. Therefore, this study was conducted to measure total phenol and total flavonoid contents of the M. sinensis accessions collected from some countries and to select the M. sinensis accessions with rich total phenol and total flavonoid contents. The results of this study can be used as basic data for future breeding. Methods and Results : This study carried out the total phenol and total flavonoid contents analysis of M. sinensis collected from different country to preserve genetic resources. Total 205 accessions of M. sinensis were collected from the USA, China, Korea, Japan, Thailand and Zambia. Their leaves were extracted with 100% methanol and concentrated at 45℃. To determine total phenol and total flavonoid contents, we measured the total phenolic content and total flavonoid content. The highest total phenolic conten t(81.04 ± 0.02 ㎎·GAE/g) showed in the accession (PMS-178) from China. The highest total flavonoid content (32.66 ± 0.01 ㎎·QE/g) showed in the accession (KMS104) from Korea. On the other hand the lowest total phenolic content (1.11 ± 0.00 ㎎·GAE/g) and the total flavonoid content (0.81 ± 0.00 ㎎·QE/g) were obtained from the accession (M. sinensis ‘Super Stripe’) from USA. Conclusion : In this study, we analysed the total phenolic and total flavonoid contents by country of origin.
        10.
        2018.10 서비스 종료(열람 제한)
        Background : Miscanthus is a species of flowering plant in the family poaceae, native to Eastern Asia throughout most of China, Japan, Taiwan and Korea. It is diploid hybrid grass used as bioenergy plant, biomass production and high quality of cellulose and ethanol production. It is an herbaceous perennial grass, growing up to 0.8 - 3.0 m tall, rarely 4.0 m, forming dense clumps from an underground rhizome. Recently, Studies on Miscanthus have been carried out on the growth characteristics and to see the antioxidant difference of Miscanthus for functional materials in various regions. Methods and Results : This study carried out the antioxidant activities of Miscanthus collected from different countries of origin. Two hundred one accessions of Miscanthus were collected in 7 countries (China, Japan, Korea, Taiwan, Thailand, U.S.A, Zambia). Initially, each leaves were extracted with 70% ethanol to determine actioxidant activty. Antioxidant activities were evaluated by DPPH and ABTS radical scavenging activity. The highest DPPH radical scavenging activity showed in the accession [M. sinensis ‘Kleine Silberspinne (little silver spider)’] from U.S.A (RC50: 10.66 ± 15.46 ㎍/㎖) and the lowest showed in the accession (M. sinensis ‘Supr Stripe’) from U.S.A (RC50: 188.03 ± 0.86 ㎍/㎖). The ABTS radical scavenging activity showed the highest in the accession (No.401: JM0310.002) from Japan (RC50: 17.97 ± 3.26 ㎍/㎖) and the lowest showed in the accession (M. sinensis ‘Supr Stripe’) from U.S.A (RC50: 416.88 ± 10.05 ㎍/㎖). Conclusion : The antioxidant activity of Miscanthus showed in average DPPH radical scavenging activity (RC50: 74.05 ± 3.74 ㎍/㎖) and ABTS radical scavenging activity (RC50: 81.46 ± 3.95 ㎍/㎖). In this study, it may be used for feed crops or food crops as a functional material.
        11.
        2018.10 서비스 종료(열람 제한)
        Background : Coffee is one of the favorite brewed drink in the world where is distributed in Latin America, Southeast Asia, Southern Asia and Africa. Coffee has an effective antioxidant ability and reported about that. In this study, it was analyzed by using high performance liquid chromatography (HPLC) to establish the method about content of caffeine, chlorogenic acid, caffeic acid and p-coumaric acid in coffee. Methods and Results : Coffee was extracted with 70% EtOH in room temperature and evaporated at 45℃. All standard and sample extract were melted and diluted with 15% MeOH. Mobile phase was prepared using water with 0.01% phosphoric acid and MeOH. All standard and sample were analyzed with gradient elution (0 min : 15% MeOH, 35 min : 30% MeOH). The chromatograms were monitored at 272 and 320 ㎚. HPLC reported linear equation that based on the calibration curve for each standard compound (caffeine : Y = 1.04e + 004X – 3.21e + 003, R2 = 0.999890. chlorogenic acid : Y = 2.86e + 004X – 8.24e + 003, R2 = 0.999891. caffeic acid : Y = 2.07e + 004X – 1.21e + 004, R2 = 0.999894. p-coumaric acid : Y = 3.24e + 004X – 1.10e + 004, R2 = 0.999897). Standard compounds were determined with qualitative and quantitative analysis. The retention time of each peak of standard compounds were separated by chromatogram. Conclusion : In this study, we determined that the analysis method of compounds in coffee. In addition, we have confirmed that separation about the retention time of each peak of caffeine and chlorogenic acid in different solvent condition depending on acid buffer. This method can be use to determine standard compound in coffee.
        12.
        2018.10 서비스 종료(열람 제한)
        Background : Oplopanax elatus Nakai. is distributed in Korea and China. In this study, we have used high performance liquid chromatography (HPLC) to compare the internal standards contents [uracil, adenosine, protocatechuic acid, syringin (eleutheroside B) and scoparone (6,7-dimethoxycoumarin)], and compared the antioxidant activity. Methods and Results : Samples were prepared two different temperature conditions (90℃ and 100℃). Total phenolic contents and total flavonoid contents were analyzed while gallic acid and quercetin were used as standard. Anti-oxidant activities were measured by determination of DPPH and reducing power assay. HPLC was reported as five standard compounds equivalent using the following linear equation based on the calibration curve. According to the results, the anti-oxidant effects of Korean O. elatus Nakai. stem extracts in 90℃ water showed more activity than that of Chinese in DPPH assay. However, the amount of internal compounds was higher in Chinese O. elatus Nakai.. The anti-oxidant effects of Korean O. elatus Nakai. stem extracts in 90℃ water showed more activity than Korean O. elatus Nakai. stem extracts in 100℃ water in DPPH assay. In this study, we had found that, at over the 100℃ temperature all the anti-oxidant effects of O. elatus Nakai. extracts were reduced. However, all five standard compounds were detected at similar value. Conclusion : These results suggests that Korean O. elatus Nakai. has higher anti-oxidant activities which can be use for bioactivity assay.
        13.
        2018.10 서비스 종료(열람 제한)
        Background : Hippophae rhamnoides L. are known for antioxidant, immunodeficiency, skin protection, influenza infection and prevention of heart disease. This study was carried out to confirm the possibility of functional food by changing the antioxidant effect using H. rhamnoides L. leaf extracts to the Gamju (sweet rice drink). Methods and Results : A total of 12 samples were made of different processes. Briefly, the H. rhamnoides L. leaf were extracted at 60℃ in two different conditions (EtOH 100%, water 100%). Gamju was fermented into three different koji (Aspergillus oryzae – red, yellow, black). In addition, The addition of H. rhamnoides L. leaf extracts were mixed in two ways (simultaneous saccharification, mixed after saccharification). Antioxidant activities were estimated by 2,2-diphenyl-1-picryl-hydrazil (DPPH) and reducing power assay. Total phenolic content (TPC) was determined by Folin-Ciocalteu method. In this study, we found that Gamju mixed with H. rhamnoides L. leaf increased antioxidant effects and TPC than the control (original Gamju). Moreover, the anti-oxidant effects of the mixed H. rhamnoides L. leaf with Gamju after saccharification exhibited more activity than simultaneous saccharification in DPPH assay. Conclusion : These results demonstrated that samples of added to the H. rhamnoides L. leaf could be use as functional food.
        14.
        2018.10 서비스 종료(열람 제한)
        Background : Arctium lappa L., Compositae plant, has been consumed as a vegetable and beverage in China, Taiwan, and Japan for a long time. Several studies have reported for the burdock to include antioxidant activity, hepato-protective efficacy, anti-inflammatory activity, anti-proliferative and apoptotic effects, anti-microbial and antiviral activity. Thus, A. lappa is considered a promising plant for the treatment of chronic diseases, such as cancer, diabetes, and AIDS and due to the increasing evidence of functional compounds contributions over a variety of health beneficial properties the A. lappa has received increasing scientific interest. The primary aim of the present study was determined antioxidant activities and analysis of standard compound in A. lappa. Methods and Results : There were five different solvent conditions (100% water, 30% EtOH, 50% EtOH, 70% EtOH, 100% EtOH), extract in the room temperature. Comparatively, 70% EtOH extract showed higher values of DPPH radical scavenging activity than others. As the increasing of EtOH percentage contents, we confirmed increase total phenol and flavonoid contents. The 2,4-di-tert- butylphenol as standard compound was detected by HPLC analysis based on the calibration curve: equation : Y = 8.17e + 003X – 1.43e + 005, R2 = 0.996227. The amount of standard compounds were similar in all each different solvent conditions, but not detected in water extract. Conclusion : These results showed that A. lappa could be used as potential materials of antioxidant, and should be need more study.
        15.
        2018.10 서비스 종료(열람 제한)
        Background : Forsythia suspensa Vahl (Oleaceae) is such an antioxidant source which is a slimbing plant widely distributed in China, Japan and Korea. The extracts of the dried fruits have been used for a long time as traditional Asian medicines to treat gonorrhea, erysipedas, inflammation and pharyngitis. It was also reported that F. suspensa was able to suppress vomiting, resist hepatic injure, inhibit of elastase activity, and exhibit diuretic, analgesic, antioxidant, anti-endotoxin and antiviral effects. This study was performed to investigate the antioxidant and whitening effect of F. suspensa extract and fractions. Methods and Results : Firstly, extract the dried F. suspensa by methanol three times at room temperature and fraction for each solvents (hexane, ethyl acetate, butanol and water). The DPPH radical scavenging activity was measured at 517 ㎚ by using a UV spectrophotometer. The gallic acid and quercetin were used as positive control of total phenol and flavonoid contents assay. Reducing power was conducted four concentration of samples and positive control, measured the absorbance at 700 ㎚. Ethyl acetate fraction showed the highest effect on DPPH radical scavenging activity, total phenol contents, and reducing power. On the other hand, the highest level of total flavonoid contents indicated in butanol fraction. The ethyl acetate fraction indicated the highest percentage of enzyme inhibition at the tested same concentration. Conclusion : These results suggest that F. suspensa extract and ethyl acetate fraction could be utilized as a antioxidant. Further biological and phytochemical study is needed.
        16.
        2018.10 서비스 종료(열람 제한)
        Background : Oplopanax elatus has many compounds such as essential oils, saponin, flavonoids, anthraquinones, and polyacetylenes etc. in all part of stems, roots, and leaves. In previous study, we isolated five compounds (uracil, adenosine, protocatechuic acid, syringin, and scoparone) from the water extract of in stems of O. elatus. In this study, we confirmed the variation of chemical constituents and antioxidant activity in leaves of O. elatus by different cultivation environment. Methods and Results : We analyzed three types of O. elatus in different cultivation environment (in vitro plant, in vivo plant and wild plant). We detected five compounds (uracil, adenosine, protocatechuic acid, syringin, and scoparone) in three types of plants by using HPLC. The contents of five compounds varied depending on the different cultivation environment. Syringin and adenosine were detected on all plants and showed different contents, respectively. We compared antioxidant activities such as total phenol contents (TPC), total flavonoid contents (TFC), DPPH and reducing power assay. The values of antioxidant activities (DPPH and reducing power) in leaves of in vitro plants were higher than other plants. Also TPC and TFC in leaves of in vitro plants showed the highest contents. Conclusion : These results could be basic data for cultivation methods about enhancement of syringin and adenosine compounds contents in leaves of O. elatus.
        17.
        2018.08 KCI 등재 서비스 종료(열람 제한)
        Background: The objective of this study was to investigate the effect of packaging material on the growth of rootstock of Liriope platyphylla. Methods and Results: This study examined the effects of two types of packaging material, LDPE (low density polyethylene) and functional film on the growth of the tubers of L. platyphylla, at 5℃. During the 16-weeks of storage period, the ratio of loss and decay of the tubers was examined at intervals of 4, 8, and 16 weeks to detect the quality of the plant. After 16 weeks of storage, the treated tubers were own. Subsequently, plant height and the number of leaves were recorded. The results revealed that functional film at 5℃ was the ideal material for the storage of L. platyphylla tubers. The rate of loss was the highest (57.42%) with a onion net and the lowest (22.12%) with a functional film. Similarly, the rate of tuber decay was highest (8.20%) using onion net and the least (4.60%) when the functional film was used. Conclusions: Thus, the use of the functional film proved to be the most effective in the storage of L. platyphylla tubers when compared with the LDPE.
        18.
        2018.05 서비스 종료(열람 제한)
        Background : Recently, wild ginseng cultured ginseng cultured in a bioreactor is mass produced using biotechnological tissue culture technology. PgTRx1 gene which is involved in the production of useful substances in fermented wild ginseng cultured root was selected and introduced into a model plant (Nicotiana benthamiana) to investigate transformation useful gene expression and possible production of useful substances. Methods and Results : The PgTRx1 gene was amplified and isolated from fermented wild ginseng cultured root. Isolated PgTRx1 gene was ligated to the plant expression vector pMBP1. Overexpression genes were recombined and cloned into E. coli. Agrobacterium tumefaciens LBA4404 was transformed, cultured A. tumefaciens LBA4404 was agro-infiltrated into a model plant for transient assay. Agro-infiltration model plants were sampled on days 0, 1, 2, and 3, and cDNA synthesis was performed after total RNA extraction. The expression level of PgTRx1 gene increased with time, and NbNR, NbHSR, NbAPx, NbSIP, NbPAL, NbPR1a and NbNOA1 genes showed a decrease in the expression level. The samples were taken to determine antioxidant activity, acetylcholine hydrolase inhibitory activity and glutamate content at 0 h, 12 h, 14 h, and 36 h. The highest antioxidant activity was observed at 24 h of sample, acetylcholine hydrolase inhibitory activity at 12 h, and glutamate at 36 h. Conclusion : The possibility of introducing the model plant of the PgTRx1 gene derived from fermented wild ginseng cultured root was confirmed. The results showed that various activities were increased with time of agro-infiltration.
        19.
        2018.05 서비스 종료(열람 제한)
        Background : Miscanthus sinensis is a diploid hybrid and a temperate, perennial, cross-pollinating grass used as bioenergy plant, biomass production and high quality cellulose and ethanol production. This study was to carried out to investigate the expression of MsCOMT gene and the variation of lignocellulosic component and phenolic compounds contents in transgenic plants. Methods and Results : Multiple bands were detected from the homologous region of the COMT gene in PCR analysis. In order to obtain more detailed results, putative transgenic lines were estimated by RT-PCR analysis to confirm the expression of mRNA. Also, analysis of the lignin, cellulose, hemicellulose, and phenolic compound contents of transgenic Miscanthus plants were performed. Total lignin content of transgenic plants was lower than that of the control plant due to reduced caffeic acid O-methyltransferase (COMT) gene expression related to lignin production. Cellulose and hemicellulose contents in transgenic plants were not increased. Variation in cellulose and hemicellulose contents had no correlation with variation in lignin content of transgenic plants. Conclusion : In conclusion, transgenic M. sinensis was obtained with down-regulated COMT gene. Lignin synthesis was decreased what offers possibility of crop modification for facilitated biofuel production.
        20.
        2018.05 서비스 종료(열람 제한)
        Background : Sorghum (Sorghum bicolor) is the fifth most important crop in the world. Although it is known as a crop with many medicinal action, Studies on sorghum breeding are inferior to other crop. Therefore, it is necessary to cultivate excellent varieties of sorghum which is stable and has excellent physiological activity. So, this study was conducted to investigate the total phenolic content and the total flavonoid content of the sorghum accessions to select the sorghum accessions with rich antioxidants. The results of this study can be used as basic data for future breeding. Methods and Results : The seeds for study were supplied from the Center for Agricultural Genetic Resources of National Institute of Agricultural Sciences. The seeds were extracted with 100% methanol and concentrated at 45℃. To determine antioxidant, we measured the total phenolic content and total flavonoid content. The highest total phenolic content showed in the accession from IT No. 155552 (55600.34 ± 17.78 ㎎·GAE/g) and the total flavonoid content showed the highest in IT No. 143716 (116563.20 ± 149.39 ㎎·QE/g). On the other hand the lowest total phenolic content showed in the accession from IT No. 135777 (20.14 ± 4.29 ㎎·GAE/g) and the total flavonoid content showed the lowest in IT No. 152910 (701.25 ± 5.53 ㎎·QE/g). Conclusion : Among 300 sorghum accessions, we analysed the total phenolic contents and total flavonoid contents.
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