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Background : Oplopanax elatus has many compounds such as essential oils, saponin, flavonoids, anthraquinones, and polyacetylenes etc. in all part of stems, roots, and leaves. In previous study, we isolated five compounds (uracil, adenosine, protocatechuic acid, syringin, and scoparone) from the water extract of in stems of O. elatus. In this study, we confirmed the variation of chemical constituents and antioxidant activity in leaves of O. elatus by different cultivation environment.
Methods and Results : We analyzed three types of O. elatus in different cultivation environment (in vitro plant, in vivo plant and wild plant). We detected five compounds (uracil, adenosine, protocatechuic acid, syringin, and scoparone) in three types of plants by using HPLC. The contents of five compounds varied depending on the different cultivation environment. Syringin and adenosine were detected on all plants and showed different contents, respectively. We compared antioxidant activities such as total phenol contents (TPC), total flavonoid contents (TFC), DPPH and reducing power assay. The values of antioxidant activities (DPPH and reducing power) in leaves of in vitro plants were higher than other plants. Also TPC and TFC in leaves of in vitro plants showed the highest contents.
Conclusion : These results could be basic data for cultivation methods about enhancement of syringin and adenosine compounds contents in leaves of O. elatus.
