논문 상세보기

노루오줌 에탄올 추출물의 산화스트레스 및 대장암 세포 억제활성 KCI 등재

Ethanol Extracts from Astilbe chinensis (Maxim.) Franch. Et Savat. Exhibit Inhibitory Activities on Oxidative Stress Generation and Viability of Human Colorectal Cancer Cells

  • 언어KOR
  • URLhttps://db.koreascholar.com/Article/Detail/346555
서비스가 종료되어 열람이 제한될 수 있습니다.
한국약용작물학회지 (Korean Journal of Medical Crop Science)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
초록

Background: Astilbe chinensis (Maxim.) Franch. Et Savat. is a plant belonging to Saxifragaceae family and contains various active ingredients including astilbin and bergenin. It has been used as a traditional Korean medicine to improve fever, pain, and cough. Recently, a number of Korean medical resources have been studied for cancer and inflammation treatment, but A. chinensis (Maxim.) Franch. Et Savat. has not yet been investigated. Consequently, this study investigated the inhibitory effect of ethanol extracts from A. chinensis (Maxim.) Franch. Et Savat. (ARE) on oxidative stress and colorectal cancer using RAW264.7 and the human colorectal cancer cell line HCT-116.
Methods and Results: In total, 500 ㎍/㎖ ARE reduced cell viability by 38.96 ± 1.32%, and increased caspase-3 activity by 133.08 ± 3.41% in HCT-116 cells. Moreover, TUNEL signaling and the early apoptosis ratio (34.56 ± 1.67%) increased by 500 ㎍/㎖ ARE treatment. H2O2-induced oxidative stress and cell death were diminished by 500 ㎍/㎖ ARE treatment through decreasing ROS (reactive oxygen species).
Conclusions: The inhibitory effects of ARE against human colorectal cancer cells is mediated by apoptosis and caspase-3 activation, and H2O2-induced ROS generation and cell death are decreased by ARE treatment in RAW264.7 cells. However, further study is required to explore how ARE treatment is involved in the signaling pathway to decrease ROS.

목차
ABSTRACT
 서 언
 재료 및 방법
  1. 실험 재료
  2. 세포배양
  3. 세포생존율 분석
  4. Caspase-3 활성도 측정
  5. 세포사멸비율 측정
  6. 활성산소종 측정
  7. TUNEL 염색
  8. 통계처리
 결과 및 고찰
  1. 노루오줌 에탄올 추출물의 대장암세포 억제효과
  2. 노루오줌 에탄올 추출물의 항산화 효과
 REFERENCES
저자
  • 노종현(한약진흥재단) | Jong Hyun Nho (National Development Institute of Korean Medicine)
  • 장지훈(한약진흥재단) | Ji Hun Jang (National Development Institute of Korean Medicine)
  • 정호경(한약진흥재단) | Ho Kyung Jung (National Development Institute of Korean Medicine)
  • 이무진(한약진흥재단) | Mu Jin Lee (National Development Institute of Korean Medicine)
  • 심미옥(한약진흥재단) | Mi Ok Sim (National Development Institute of Korean Medicine)
  • 정다은(한약진흥재단) | Da Eun Jeong (National Development Institute of Korean Medicine)
  • 조현우(한약진흥재단) | Hyun Woo Cho (National Development Institute of Korean Medicine) Corresponding author