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A chitinase with two catalytic domains is required for higher ordered chitin fiber organization for the cuticular extracellular matrix of a beetle

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  • URLhttps://db.koreascholar.com/Article/Detail/346798
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한국응용곤충학회 (Korean Society Of Applied Entomology)
초록

To accommodate growth, insects must periodically replace their chitin/protein-rich cuticles in a process called “molting or ecdysis”. During each molt cycle, a new cuticle is deposited simultaneously with degradation of the chitinous procuticle of the old one by molting fluid enzymes including epidermal chitinases. Here, we demonstrated a novel role for an endochitinase, TcCHT7, from the red flour beetle, Tribolium castaneum, belonging to a subfamily (Group III) that contain two catalytic domains, in organizing chitin in the newly forming cuticle rather than in degrading chitin present in the prior one. The conservation of CHT7-like proteins among many insect and other arthropod species indicates a critical role for the Group III class of chitinases in the higher ordered organization of chitin fibers for development of the structural integrity of many invertebrate exoskeletons.

저자
  • Mi Young Noh(Department of Forestry, Chonnam National University, Republic of Korea)
  • Yasuyuki Arakane(Department of Applied Biology, Chonnam National University, Republic of Korea)