Azuki bean beetle (ABB), Callosobruchus chinensis (L.) is a field-to-storage pest of legumes and its female produces sex pheromone with two isomeric components, 2Z-homofarnesal and 2E-homofarnesal. Two day old virgin adults were exposed to 0, 200, 300, 400, 500 & 600 Gy gamma radiation and effect on longevity, fecundity, sterility and pheromone production were studied. Longevity (female; P < 0.001, male; P < 0.001) and fecundity (P < 0.001) were dose-dependently affected by the gamma irradiation. Both adults were totally sterilized by the tested doses of gamma irradiation as depicted by the null hatchability of laid eggs. Gas chromatography-mass spectrometry for solid phase micro extraction revealed that both of the pheromone components were significantly but not completely reduced by 300 Gy. It is a pre-requisite for a successful sterile insect technology that the sterility of ABB is induced without the total disruption of calling behavior.

• Kashinath Chiluwal(Division of Applied Life Science (BK21+ Program)/ Institute of Agriculture and Life Science, Gyeongsang National University, Korea, Nepal Agricultural Research Council (NARC), Kathmandu, Nepal)
• Junheon Kim(Forest Insect Pests and Disease Division, National Institute of Forest Science, Seoul, Korea)
• Soon Do Bae(Department of Southern Area Crop Science, National Institute of Crop Science, Miryang, Korea)
• Gwang Hyun Roh(Agroecosystem Management Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Nebraska, United States)
• Hae Joon Park(Biotechnology Division, Advanced Radiation Technology Institute, KAERI, Jeouneup-si, Korea)
• Chung Gyoo Park(Division of Applied Life Science (BK21+ Program)/ Institute of Agriculture and Life Science, Gyeongsang National University, Korea)