Salmonella spp. and Brucella spp. have caused a considerable disease of farmed animals and economic loss in animal farming and food industry. In this study, the disinfection efficacy of Vital-Oxidel®, a commercial disinfectant, composed to chlorine dioxide, betaine hydrochloride, and propylene glycol was evaluated against S. typhimurium and Brucella ovis. A bactericidal efficacy test by broth dilution method was used to determine the lowest effective dilution of the disinfectant following exposure to test bacteria for 30 min at 4oC. Vital-Oixdel® and test bacteria were diluted with distilled water (DW), hard water (HW) or organic matter suspension (OM) according to treatment condition. On OM condition, the bactericidal activity of Vital-Oixdel® against S. typhimurium and Brucella ovis was lowered compared to that on HW condition. As Vital-Oxidel® possesses bactericidal efficacy against animal pathogenic bacteria such as S. typhimurium and Brucella ovis, this disinfectant solution can be used to control the spread of bacterial diseases.