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        검색결과 254

        1.
        2024.04 구독 인증기관·개인회원 무료
        고자리꽃파리는 양파 및 마늘 등 백합과 Allium 속에 속하는 농작물에 중요한 해충으로 전 세계적으로 온대지역에 서 경제적 해충으로 취급하고 있다. 본 연구에서는 기존 자료를 바탕으로 월동번데기의 성충으로 우화모델를 작성하 고 포장 실측자료와 비교하여 평가하였다. 월동번데기 발육모형으로 선형과 비선형모형을 작성하고 발육기간 분포 모형과 결합하여 예찰모형을 작성하였다. 비선형발육모형 작성시 3-매개변수 락틴모형 적용뿐만 아니라 4-매개변 수 모형의 마지막 변수 값을 선형모형의 절편값으로 대체하여 저온에서 선형성이 강화도록 변형시켰다. 성충우화 50% 예측에서 일일평균온도를 이용하는 경우 적산온도 모형을 비롯하여 발육률 적산모형(선형식 및 비선형식) 모두 실측치와 큰 차이가 있었다. 시간별온도를 입력값으로 한 경우 3-매개변수 모형을 제외한 사인곡선 적산온도 모형, 선형 발육률 적산모형, 4-매개변수 비선형 발육률 적산모형의 평균편차는 3일과 차이가 없었다. 최종적으로 선형모형 및 4-매개변수 비선형모형을 바탕으로 시간별온도자료를 이용한 발육률 적산모형은 선발하였다. 그 결과 선형 발육률 적산모형이 두 포장적합 집단(1984, 1987)에서 실측일과 편차가 3일과 차이가 없었다. 비선형 발육률 적산모형은 1984년 적합은 0.8일 편차로 정확하였으나 1987년 집단에서 평균편차가 6.5일로 다소 증가하였다.
        2.
        2024.04 구독 인증기관·개인회원 무료
        The Japanese pine sawyer (JPS), Monochamus alternatus Hope, is an important vector of the pine wood nematode, Bursaphelenchus xylophilus (Steiner and Buhrer), which causes pine wilt disease. JPS selects a characteristic life cycle of a four- or five-instar pathway, which indicates the number of instars before pupation. In this study, we investigated the potential presence of a three-instar pathway and sought to determine the minimum larval age required for JPS to construct a pupal chamber. The results showed that no third instars made pupal chambers, suggesting that there is no three-instar pathway. The initiation time of digging the tunnel to make the pupal chamber ranged widely from Julian date 274 (30 September) to 332 (27 November). This timing became an average age stage of 4.8 instar, which equals an 80% completion of the fourth instar. Thus, the minimum larval age at which JPS constructs the tunnel to pupate the next year is estimated to be late in the fourth larval instar. Further, we discuss the diapause ecology in relation to the larval development of JPS in different thermal environments. Tentatively, we suggest that the regulation of diapause induction in JPS involves a dual process of reaching a critical developmental stage and stimulus (tentatively a cold temperature). This hypothesis will be helpful for future studies of diapause mechanisms and the selection of the instar-pathway in JPS and related species.
        3.
        2024.04 구독 인증기관·개인회원 무료
        본 연구는 검거세미밤나방(Agrotis ipsilon) 성페로몬 트랩에 혼재하여 유살되는 은무늬밤나방아과 형태적 분류와 동정법 수립을 위해, 날개 무늬의 형태계측학 분석을 실시하였다. 은무늬밤나방아과 개체는 2023년 11월 부터 12월까지 제주도 애월읍 일대에서 채집되었으며, 콩 해충으로 알려진 콩은무늬밤나방(Ctenoplusia agnata) 을 비롯하여, 다양한 농작물을 가해하는 것으로 알려진 붉은금무늬밤나방(Chrysodeixis eriosoma)의 수컷 성충이 포획되었다. 앞날개의 형태 및 무늬를 가지고 현장에서 쉽게 동정할 수 있는 형태적 특징을 도출하기 위하여, 각 성충 개체의 앞날개를 잘라 현미경 카메라로 촬영하고, 앞날개의 내횡선, 아외연선, 반점 크기 등 15개의 형질 을 측정하였다. 또한 각 형질 간의 상관관계를 분석하였으며 빈도분포를 통하여 두 종간 분리되는 형질을 파악하 였다. 최종적으로 다변량 분석법을 적용하여 두 집단이 어떻게 군집을 이루는지 분석하고, 날개형태만으로 붉은 금무늬밤나방과 콩은무늬밤나방을 구분할 수 있는 방안을 제시하였다.
        4.
        2023.12 KCI 등재 구독 인증기관 무료, 개인회원 유료
        This study was conducted to reset the maximum residue limit (MRL) for didecyldimethylammonium chloride (DDAC) in broiler chickens. The disinfectant containing DDAC (10%, w/w) was diluted 160 times and evenly sprayed on the bodies of twenty-four broiler chickens at a rate of 15 mL per day per bird for 7 days. After the disinfectant treatment, tissue samples were collected from six broiler chickens at 0.25, 1, 3 and 5 days, respectively. Residual DDAC concentrations in poultry tissues were determined using LC-MS/MS. Correlation coefficient (0.99 >), the limits quantification (LOQ, 2.0~10.0 μg/kg), recoveries (86.9~118.6%), and coefficient of variations (<19.98%) were satisfied the validation criteria of Korean Ministry of Food and Drug Safety. In all tissues except for liver, DDAC was detected more than LOQ at 5 days after the disinfectant treatment. In liver tissues, DDAC was detected more than LOQ at 3 days after treatment. According to the European Medicines Agency’s guideline on determination of withdrawal periods, withdrawal period of DDAC in poultry tissues was established to 26 days. In conclusion, the developed analytical method is sensitive and reliable for detecting DDAC in poultry tissues. When DDAC disinfectant is sprayed on a poultry house in the presence of broiler chickens, it is necessary to keep the disinfectant from contacting the body of the livestock.
        4,000원
        5.
        2023.12 KCI 등재 구독 인증기관 무료, 개인회원 유료
        The primary therapeutic approach for Brucella species infections has mainly been based on antibiotic treatment. However, the development of vaccines for brucellosis control remains controversial. Furthermore, there is currently no licensed vaccine available for human brucellosis. This study aims to evaluate the effect of a combination of recombinant protein vaccines against Brucella (B.) abortus infection using a mouse model. Two B. abortus genes, namely dapB and gpm, were cloned and expressed in competent Escherichia (E.) coli DH5α using the pCold-TF vector. Successfully cloned vectors were subjected to PCR amplification using specific primer pairs. The apparent sizes of dapB and gpm were detected at 807 bp and 621 bp, respectively. Besides, the purified recombinant proteins dapB and gpm were detected using SDS-PAGE electrophoresis with correct sizes of 82.86 kDa and 87.61 kDa, respectively. These recombinant proteins were used to immunize mice as a combined subunit vaccine (CSV) to elicit host immunity against B. abortus infection. Mice immunized with CSV exhibited increased proliferation of CD4+ and/or CD8+ T cells at week 7th and 9th before sacrifice, in comparison to the control group. Notably, CSV immunization showed a significant decrease in bacterial burden in the spleen compared to the control group. Altogether, CSV using dapB and gpm induced host adaptive immune response against Brucella infection, suggesting its potential as an effective new subunit vaccine candidate.
        4,000원
        6.
        2023.12 KCI 등재 구독 인증기관 무료, 개인회원 유료
        This study evaluated the fungicidal efficacy of weakly acidic hypochlorous acid water (WAHW) against Microsporum canis (M. canis) and its therapeutic effect on M. canis-infected mouse skin. WAHW was produced by a WAHW generation module. A fungicidal efficacy test by the broth dilution method was used to determine the lowest effective concentration of the WAHW. The lowest effective concentration of WAHW was less than 10 ppm. For T-1, T-2, and T-3, 30 ppm of WAHW was applied to the infected skin once, twice, and three times a day, respectively, and for T-4, 50 ppm of WAHW was applied once a day. On the 3rd day after the initiation of treatment, skin scores in all of the WAHW-treated groups were significantly decreased compared to those in the positive control group (PC) (p<0.05), and there were no significant differences compared to the normal control (NC). The area of the infected skin in all of the WAHW-treated groups was significantly decreased compared to PC from the first day after the initiation of WAHW treatment (p<0.05). The results showed that WAHW had a fungicidal efficacy on M. canis at less than 10 ppm, and it was effective in improving skin symptoms when 30 ppm of WAHW was applied to the M. canis-infected area once a day for five days or 50 ppm of WAHW was applied once a day.
        4,000원
        7.
        2023.10 구독 인증기관·개인회원 무료
        농가에서 곤충자원을 활용한 치유농업프로그램의 개발 요구가 증가함에 따라 기존 곤충 돌보기 중심의 치유프로그램과 더불어 활용할 수 있는 야간 곤충채집 활동을 치유농업프로그램으로 개발하 고 시범 적용하였다. 야간곤충채집 활동은 대학생 9명에게 사전에 참여 동의를 획득하고, 전남대학교 학술림(장성)에서 1박 2일간 진행하였다. 18시부터 24시까지 야간 채집 활동과 익일 오전에는 채집한 곤충을 이용한 곤충 표본 제작 활동으로 프로그램을 구성하였다. 프로그램의 효과 분석을 위해 사전 (활동 전), 중간(채집활동 직후), 사후(표본제작 직후)에 뇌파, 맥파 검사 그리고 설문조사를 실시하였 다. 뇌파 분석 결과, 야간 채집활동 직후 뇌파의 기초율동이 향상(10.67→11.44)하였다(p<0.05). 요인 분석을 통한 스트레스 분석 결과, 내적 스트레스는 3.22(사전)→2.96(사후)로 감소(p<0.1)하였고, 문제 수행능력 관련 스트레스는 2.92(중간)→2.70(사후)로 감소하였다. 이러한 결과는 야간 채집 활동 속에 서 이용되는 근육의 움직임이 뇌 기능 향상과 스트레스 감소에 영향을 줄 것으로 생각된다. 참여자가 적은 연구의 한계를 극복하기 위해 향후 연령대별, 성별 등 다양한 참여자를 대상으로 추가적인 채집 프로그램의 개발 및 적용이 필요하다.
        8.
        2023.10 구독 인증기관·개인회원 무료
        왕담배나방은 기주범위가 넓은 다식성으로 토마토, 옥수수, 담배, 해바라기, 땅콩, 면화, 콩 등 다양한 작물에 피해를 주는 해충이며, 장거리 이동성 해충으로도 유명하다. 본 연구에서는 기존 자료를 바탕으로 월동번데기의 성충으로 우화모델(2종)를 작성하고 포장 실측자료와 비교하여 평가하였다. 월동 번데기는 휴면을 종료한 후 안점소실 과정을 거쳐 정상의 번데기로 이행되고 비로소 성충으로 우화하는 것으로 가정하였다. 첫 번째 모형(모 형 1)은 2단계 휴면후발육 우화모형으로 안점소실에 필요한 적산온도 59.9DD (발육영점온도 15.9℃)와 번데기 발육완료에 필요한 192.3DD (발육영점온도 10.2℃)로 구성하였다. 두 번째 모형(모형 2)은 온도자극 우화모형으 로 17.63℃의 온도자극 이후에 번데기 발육을 개시하는 모형이었다. 적산온도는 단순 평균온도법과 사인곡선법 으로 계산하여 비교하였다. 포장자료와 비교 결과 모형 1에 사인곡선법을 적용했을 때 실측치와 편차가 3일보다 작거나 차이가 없었다. 반면 사인곡선법을 적용한 모형 2는 3~6.5일의 편차가 있었다. 평균온도법을 적용한 경우 두 모형 모두 실측치와 편차가 증가하였다. 모형을 활용하여 예측 우화시기와 트랩유살자료를 비교하여 왕담배 나방의 비래 가능성에 대하여 고찰하였다.
        9.
        2023.10 구독 인증기관·개인회원 무료
        지난 2022년 제주도 애월읍 일대에서 콩 해충으로 알려진 콩은무늬밤나방(Ctenoplusia agnata) 성충이 검거세 미밤나방(Agrotis ipsilon)의 성페로몬 트랩에 대량으로 포획되었다. 검거세미밤나방 트랩은 목적 해충에 대한 포획 효율을 조사하기 위해 세 구성 성분, (Z)-7-dodecenyl acetate, (Z)-9-tetradecenyl acetate를 3:1 비율로 고정하고 (Z)-11-hexadecenyl acetate를 0, 1, 6, 10, 15로 각각 비율을 달리한 미끼를 사용하였다. 각 조성별 콩은무늬밤나방 성충 포획수를 비교한 결과, (Z)-11-hexadecenyl acetate가 첨가되지 않은 트랩에서 주당 평균 약 17.96마리로, 가장 많은 수의 개체가 포획된 것으로 확인되었다. 반면, (Z)-11-hexadecenyl acetate가 가장 많이 함유된 트랩에서 주당 평균 약 2.5마리로 가장 적은 개체가 포획된 것으로 파악되었다. 이에 (Z)-11-hexadecenyl acetate의 비율이 증가할 수록, 포획되는 콩은무늬밤나방의 개체 수가 감소되는 것을 확인할 수 있었다. 검거세미밤나방 미끼의 주성분인 Z)-7-dodecenyl acetate는 기존의 콩은무늬밤나방 유인 성분 중 하나이기도 하여 해당 성분의 구성비가 유인에 영향을 미쳤다는 것을 예측할 수 있다. 추후에 해당 트랩들과 시판 중인 콩은무늬밤나방 성페로몬 트랩을 설치하 여 포획 양상을 비교할 필요성이 요구된다.
        14.
        2023.09 KCI 등재 구독 인증기관 무료, 개인회원 유료
        Extensive research and testing continue to be conducted for the development of vaccines targeting zoonotic diseases such as brucellosis. In this study, the potential of the DapB as a recombinant protein vaccine to effectively combat Brucella abortus 544 infection in BALB/c mice was evaluated. Western blotting assay results showed that recombinant protein DapB reacted with Brucella-positive serum, indicating its potential immunoreactivity. In vivo results showed that the peripheral blood CD4+ and CD8+ T cell population significantly increased in the DapB-immunized mice group after the first, second and third blood collection, compared to the control group that received PBS. Additionally, at the fourth blood collection, an increase in CD4+ T cell activation was observed in three vaccination groups compared to PBS negative control group. These results indicate the potential of DapB in stimulating cellular immunity. Fourteen days after infection, the bacterial load in the spleen was evaluated. The reduction in bacterial replication in the spleen by both DapB and RB51 highlights their protective efficacy against Brucella infection. These findings contribute to the ongoing efforts in developing effective vaccines against brucellosis and provide valuable insights for further research in this field.
        4,000원
        15.
        2023.09 KCI 등재 구독 인증기관 무료, 개인회원 유료
        This study investigated ethopabate (EPB) residues in edible tissues of broiler chickens given in drinking water and established the withdrawal time (WT) of EPB in poultry tissues. Twenty-four healthy Ross broiler chickens were orally administered with EPB at the concentration of 3.8 mg/L for 14 days (EPB-1, n=24) and 15.2 mg/L for 7 days (EPB-2, n=24) through drinking water, respectively. After the drug treatment, tissue samples were collected from six broiler chickens at 0, 1, 3, and 5 days, respectively. EPB residue concentrations in poultry tissues were determined using LC-MS/MS. Correlation coefficient values ranged from 0.9980 to 0.9998, and the limits of detection and quantification (LOQ) were 0.03~0.09 and 0.1~0.3 μg/kg, respectively. Mean recoveries in muscle, liver, kidney and skin/fat tissues were 95.9~109.8, 108.7~115.3, 89.9~96.6 and 86.7~96.8%, respectively, and coefficient of variations were less than 17.11%. At the end of the drug-administration period (0 day), EPB was detected at levels under the LOQ in all tissues from both the EPB-1 and EPB-2 groups. According to the results of EPB residue in Ross broiler tissues, withdrawal periods of both EPB-1 and EPB-2 in poultry tissues were established to 0 day. In conclusion, the developed analytical method is suitable for the detection of EPB in poultry tissues, and the estimated WT of EPB in poultry tissues will contribute to ensuring the safety of Ross broiler chickens.
        4,000원
        16.
        2023.06 KCI 등재 구독 인증기관 무료, 개인회원 유료
        With about 80% of the global economy expected to shift to the global market by 2030, exports of reverse direct purchase products, in which foreign consumers purchase products from online shopping malls in Korea, are growing 55% annually. As of 2021, sales of reverse direct purchases in South Korea increased 50.6% from the previous year, surpassing 40 million. In order for domestic SMEs(Small and medium sized enterprises) to enter overseas markets, it is important to come up with export strategies based on various market analysis information, but for domestic small and medium-sized sellers, entry barriers are high, such as lack of information on overseas markets and difficulty in selecting local preferred products and determining competitive sales prices. This study develops an AI-based product recommendation and sales price estimation model to collect and analyze global shopping malls and product trends to provide marketing information that presents promising and appropriate product sales prices to small and medium-sized sellers who have difficulty collecting global market information. The product recommendation model is based on the LTR (Learning To Rank) methodology. As a result of comparing performance with nDCG, the Pair-wise-based XGBoost-LambdaMART Model was measured to be excellent. The sales price estimation model uses a regression algorithm. According to the R-Squared value, the Light Gradient Boosting Machine performs best in this model.
        4,000원
        17.
        2023.06 KCI 등재 구독 인증기관 무료, 개인회원 유료
        In the era of the 4th Industrial Revolution, Logistic 4.0 using data-based technologies such as IoT, Bigdata, and AI is a keystone to logistics intelligence. In particular, the AI technology such as prognostics and health management for the maintenance of logistics facilities is being in the spotlight. In order to ensure the reliability of the facilities, Time-Based Maintenance (TBM) can be performed in every certain period of time, but this causes excessive maintenance costs and has limitations in preventing sudden failures and accidents. On the other hand, the predictive maintenance using AI fault diagnosis model can do not only overcome the limitation of TBM by automatically detecting abnormalities in logistics facilities, but also offer more advantages by predicting future failures and allowing proactive measures to ensure stable and reliable system management. In order to train and predict with AI machine learning model, data needs to be collected, processed, and analyzed. In this study, we have develop a system that utilizes an AI detection model that can detect abnormalities of logistics rotational equipment and diagnose their fault types. In the discussion, we will explain the entire experimental processes : experimental design, data collection procedure, signal processing methods, feature analysis methods, and the model development.
        4,000원
        18.
        2023.06 KCI 등재 구독 인증기관 무료, 개인회원 유료
        From 2020, Korean Animal and Plant Quarantine Agency has reset the withdrawal time (WT) for veterinary drugs typically used in livestock in preparation for the introduction of positive list system (PLS) program in 2024. This study was conducted to reset the MRL for amprolium (APL) in broiler chickens as a part of PLS program introduction. Forty-eight healthy Ross broiler chickens were orally administered with APL at the concentration of 60 mg/L (APL-1, n=24) for 14 days and 240 mg/L (APL-2, n=24) for 7 days through drinking water, respectively. After the drug treatment, tissue samples were collected from six broiler chickens at 0, 1, 3 and 5 days, respectively. Residual APL concentrations in poultry tissues were determined using LC-MS/MS. Correlation coefficient (0.99 >), the limits quantification (LOQ, 0.3~5.0 μg/kg), recoveries (81.5~112.4%), and coefficient of variations (<15.5%) were satisfied the validation criteria of Korean Ministry of Food and Drug Safety. In APL-1, APL in all tissues except for kidney was detected less than LOQ at 3 days after drug treatment. In APL-2, APL in liver and kidney was detected more than LOQ at 5 days after treatment. According to the European Medicines Agency’s guideline on determination of withdrawal periods, withdrawal periods of APL-1 and APL-2 in poultry tissues were established to 3 and 2 days, respectively. In conclusion, the developed analytical method is sensitive and reliable for detecting APL in poultry tissues. The estimated WT of APL in poultry tissues is longer than the current WT recommendation of 2 days for APL in broiler chickens.
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