This study was designed to investigate the effect of fucoidan on the activation of macrophage and on induction of apoptosis in AGS cell. To measure the activity of macrophages, NO and TNF-α assays were performed in Raw 264.7 cell. Treatment with fucoidan significantly increased production of NO and TNF-α, indicating activation of macrophages. The result of MTT assay shows that cell viability was significantly decreased in a dose and time-dependent manner. Fucoidan increased to enhance mitochondrial membrane permeability, as well as the cytochrome c release from the mitochondria. Fucoidan decreased Bcl-2 and XIAP expression, whereas the expression of Bax was increased in a time-dependent manner compared to the control. In addition, the active forms of caspase-9 were increased, and the inactivation of Akt was decreased in a time-dependent manner. Caspase inhibitor, z-VADFMK, canceled the apoptosis of fucoidan, expression of Bax and caspase-9 were decrease. These results indicate that fucoidan induces activation of macrophage and apoptosis through activation of caspase on AGS cell.

ABSTRACT
 서 론
 재료 및 방법
  세포배양 및 시험물질
  Fucoidan에 의한 세포증식 효과 측정
  대식세포 내 일산화질소의 측정
  대식세포 내 종양괴사인자-알파의 측정
  DAPI 염색
  Apoptosis 측정
  웨스턴 블로팅
  미토콘드리아의 전압 측정
  통계학적 처리
 결과 및 고찰
  대식세포에 대한 Fucoidan의 세포독성능 평가
  Fucoidan에 의한 대식세포의 NO 및 TNF-α 분비
  Fucoidan에 의한 AGS 세포의 성장에 미치는 영향
  Fucoidan에 의한 apoptosis 유도
  Fucoidan에 의한 세포 내 신호경로
  Fucoidan에 의한 세포 내 Akt의 인산화
  Caspase 억제제가 AGS 세포주의 증식에 미치는 영향
 요 약
 참고문헌

• 안인정(공주대학교 특수동물학과, Department of Companion and Laboratory Animal Science, Kongju National University) | In-Jung An
• 조성대(전북대학교 구강병리학교실, Department of Oral Pathology, School of Dentistry, Institute of Oral Bioscience, Chonbuk National University) | Sung-Dae Cho
• 권중기(공주대학교 특수동물학과, Department of Companion and Laboratory Animal Science, Kongju National University) | Jung-Ki Kwon
• 김혜리(공주대학교 특수동물학과, Department of Companion and Laboratory Animal Science, Kongju National University) | Hye-Ri Kim
• 유현주(전북대학교 구강병리학교실, Department of Oral Pathology, School of Dentistry, Institute of Oral Bioscience, Chonbuk National University) | Hyun-Ju Yu
• 정지윤(공주대학교 특수동물학과, Department of Companion and Laboratory Animal Science, Kongju National University) | Ji-Youn Jung Correspondence to