In this paper, the combustion characteristics of constant volume combustion chamber(CVCC) were experimentally investigated when biodiesel is mixed with pure gasoline. The experiment was performed on two gasoline biodiesel samples designated by GB05 and GB20 which is mixed with 5% and 20% biodiesel respectively. It was confirmed that the ignition delay time decreases as the temperature of injection engine increases due to ignition delay. Also, it was shown that the ignition delay time decreases as the biodisel mixing ratio increases from 5% to 20%.