Since 2011, a total length of national highway in Korea is 13,797km and 97.6% of total length of national highway system is paved. Particularly, most of national highway has been paved as asphalt concrete pavement. Currently, many maintenance techniques, such as overlay and surface treatments, are applied on existing pavements depending on the failure levels of pavements. One of maintenance techniques is a hot in-place recycling (HIR) that shows features of cost effective and better performance because of the use of 100% recycling of reclaimed asphalt of existing pavement. HIR is widely used in Europe countries and the U.S. It is recommended to rehabilitate the old and distressed asphalt pavement because it is suitable from the environmental perspective and convenient to use when a road is paved. Since 2002, HIR as a maintenance method has been used to rehabilitate the distressed asphalt pavement in National highway in Korea. Recently, a new HIR system, which consists of three pre-heaters, two recycler and one paver, is introduced in maintenance project in National highway. In this paper, HIR mixtures collected from a new HIR system in the field are tested to evaluate their performance in the laboratory test. First, the reclaimed asphalt (RAP) materials and HIR mixtures are collected in the field project site. Second, the quality evaluation of RAP materials, HIR mixtures and rejuvenator are conducted in the laboratory. Test results of RAP materials and HIR mixtures are satisfied with national specification. Rejuvenator used in the project site is classified as RA-5. Based on the limited teste results, it would be concluded that a new HIR system could be produced a satisfied HIR mixtures in the maintenance projects of National highway.