전분의 사용원료를 확인하는 방법은 전분입자의 크기 또는 형태 등으로 분류하는 이화학적인 방법이 연구되었으나 원료별 또는 동일한 원료라도 품종에 따른 차이점으로 인하여 명확하게 확인하기 어려운 단점이 있어 유전자분석법을 시도하였다. 시료는 고구마 전분, 감자 전분, 옥수수 전분 및 타피오카 전분 등 총 11종을 사용하였으며, 유전자추출은 DNeasy plant mini 키트, magnetic DNA purification system 및 CTAB 방법으로 하였으며 추출유전자의 증폭을 위하여 WGA 키트로 처리하였다. 그리고 고구마, 감자, 옥수수 및 타피오카 검출을 위한 유전자 부위는 SSR (simple sequence repeat, ib-286-F/ib-286-R), 자당합성효소 (potato sucrose synthase, Pss 01n-5'/Pss 01n-3'), 전분합성효소(starch synthase, SSllb 3-5'/SSllb 3-3') 및 SSR (SSRY26- F/SSRY26-R)를 각각 사용하였다. 그 결과 대부분의 경우 WGA를 처리한 경우에는 사용원료의 확인이 가능하였다.

Identification of main ingredients in starches has been investigated using physicochemical analysis method mainly. However, physicochemical properties such as particle size have limitations in determining the differences among mixed starches. Therefore, we developed a molecular biological method to identify materials used in starch, as a sample, 11 kinds of starches including sweet potato starch, potato starch, corn starch, and tapioca starch. DNeasy plant mini kit, magnetic DNA purification system, and CTAB methods were used to extract DNA from samples. After gene extraction, whole genome amplification (WGA) was performed to amplify the extracted DNA. Species- specific primers were used as followings: ib-286-F/ib-286-R (105 bp), Pss 01n-5'/Pss 01n-3' (216 bp), SS11b 3- 5'/SS11b 3-3' (114 bp), and SSRY26-F/SSRY26-R (121 bp) gene for sweet potato, potato, corn, and tapioca, respectively. In this study, we could confirm the main ingredients using WGA and PCR method.

ABSTRACT
 서 론
 재료 및 방법
  시약 및 검체 구입
  유전자 추출 및 WGA kit 사용
  전분의 원료 확인을 위한 목표 유전자 및 프라이머
  Polymerase Chain Reaction (PCR) 반응액 조성 및 반응조건
  결과확인
 결과 및 고찰
  유전자추출 방법별 추출효율 비교 및 PCR 수행
  감자 전분의 PCR 결과 분석
  고구마 전분의 PCR 결과 분석
  옥수수 전분 및 타피오카 전분의 PCR 결과 분석
  전분의 사용원료 확인에 적용성 검토
 요 약
 참고문헌

• 박용춘(식품의약품안전처 식품의약품안전평가원 식품위해평가부 신종유해물질팀) | Yong-Chjun Park (New Hazardous Substance Team, Food Safety Evaluation Department, National Institute of Food & Drug Safety Evaluation, Ministry of Food & Drug Safety) Correspondence to
• 김미라(식품의약품안전처 식품의약품안전평가원 식품위해평가부 신종유해물질팀) | Mi-Ra Kim (New Hazardous Substance Team, Food Safety Evaluation Department, National Institute of Food & Drug Safety Evaluation, Ministry of Food & Drug Safety)
• 김용상(식품의약품안전처 식품의약품안전평가원 식품위해평가부 신종유해물질팀) | Yong-Sang Kim (New Hazardous Substance Team, Food Safety Evaluation Department, National Institute of Food & Drug Safety Evaluation, Ministry of Food & Drug Safety)
• 이호연(식품의약품안전처 식품의약품안전평가원 식품위해평가부 신종유해물질팀) | Ho-Yeon Lee (New Hazardous Substance Team, Food Safety Evaluation Department, National Institute of Food & Drug Safety Evaluation, Ministry of Food & Drug Safety)
• 김규현(식품의약품안전처 식품의약품안전평가원 식품위해평가부 신종유해물질팀) | Kyu-Heon Kim (New Hazardous Substance Team, Food Safety Evaluation Department, National Institute of Food & Drug Safety Evaluation, Ministry of Food & Drug Safety)
• 이재황(식품의약품안전처 식품의약품안전평가원 식품위해평가부 신종유해물질팀) | Jae-Hwang Lee (New Hazardous Substance Team, Food Safety Evaluation Department, National Institute of Food & Drug Safety Evaluation, Ministry of Food & Drug Safety)
• 김재이(식품의약품안전처 식품의약품안전평가원 식품위해평가부 신종유해물질팀) | Jae-i Kim (New Hazardous Substance Team, Food Safety Evaluation Department, National Institute of Food & Drug Safety Evaluation, Ministry of Food & Drug Safety)
• 이상재(식품의약품안전처 식품의약품안전평가원 식품위해평가부 신종유해물질팀) | Sang-Jae Lee (New Hazardous Substance Team, Food Safety Evaluation Department, National Institute of Food & Drug Safety Evaluation, Ministry of Food & Drug Safety)
• 이화정(식품의약품안전처 식품의약품안전평가원 식품위해평가부 신종유해물질팀) | Hwa-Jung Lee (New Hazardous Substance Team, Food Safety Evaluation Department, National Institute of Food & Drug Safety Evaluation, Ministry of Food & Drug Safety)