Antibiotic Detection Kit (Combination I), a lateral flow immunoassay (LFIA) developed for the detection of antibiotic residues in milk, was utilized for the analysis of antibiotic residues in the muscle tissue of olive flounder. After 60-min treatment by dipping in water dosed with ampicillin (200-g/ton water), the residue depletion of ampicillin was investigated in 25 cultured olive flounder (Paralichthys olivaceus). Muscles of fish were sampled on the 1st, 2nd, 3rd, 4th and 5th day after drug treatment. The concentration of ampicillin in the muscle was determined by LFIA. The absorbance ratio of the sample to the control blank (Bs/Bo) was employed as an index to determine the muscle residues in olive flounder. To investigate the recovery rate, standard solutions were added to muscle samples to give final concentrations in the muscle of 4 and 8 ng/ml. The recovery rates of all spiked samples were > 96% of the spiked value. Ampicillin was detected in the muscle of fish treated with the drug until the 2nd day of the withdrawal period. The present study showed that the LFIA can be easily adopted to predict ampicillin residues in tissue of farmed fishes.

ABSTRACT
 Introduction
 Materials and Methods
  Regents and immunoassay kit
  Drug treatment and collection of samples
  Analysis of ampicillin residues
  Preparation of standard curve
  Recovery test
 Results
 References

• Chun Nam Cha(Engineering Research Institute, Department of Industrial Systems Engineering, Gyeongsang National University)
• Eun-Ah Yu(Tongyeong National Quarantine Station, Ministry of Health & Welfare)
• Min Jung Shin(3M Health Care Business Group Laboratory, 3M Korea)
• Eun Kee Park(Department of Medical Humanities and Social Medicine, College of Medicine, Kosin University)
• Hyunju Choi(Elderly Life Redesign Institute, Department of Biomedical Laboratory Science, Inje University)
• Suk Kim(Research Institute of Life Sciences, College of Veterinary Medicine, Gyeongsang National University)
• Hu Jang Lee(Research Institute of Life Sciences, College of Veterinary Medicine, Gyeongsang National University) Correspondence to