The aim of the present work was to develop simultaneous methods of quantification of carazolol, azaperone, and azaperol residues in livestock and fishery products using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Samples were extracted from beef, pork, chicken, egg, milk and shrimp using acetonitrile (ACN); while flat fish and eel were extracted using 80% ACN. For purification, ACN saturated n-hexane was used to remove fat composition. The standard calibration curves showed good linearity as correlation coefficients; r 2 was > 0.99. Average recoveries expressed were within the range of 67.9-105% for samples fortified at three different levels (0.5 × MRL, 1 × MRL and 2 × MRL). The correlation coefficient expressed as precision was within the range of 0.55- 7.93%. The limit of quantification (LOQ) was 0.0002-0.002 mg/kg. The proposed analytical method showed high accuracy and acceptable sensitivity based on Codex guideline requirements (CAC/GL71-2009). This method can be used to analyze the residue of carazolol, azaperone, and azaperol in livestock and fishery products.

ABSTRACT
 Materials and Methods
  시약 및 재료
  표준원액 및 표준용액의 조제
  시료 전처리 방법
  시료분석 기기조건
  분석법 검정 실험
 Results and Discussion
  LC-MS/MS를 이용한 분석법 및 최적조건확립
  추출 및 정제조건의 최적화
  분석방법의 유효성평가
 국문요약
 References

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• 강희승(식품의약품안전처 식품의약품안전평가원 식품위해평가부 잔류물질과) | Hui-Seung Kang (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation) Correspondence to
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• 천소영(식품의약품안전처 식품의약품안전평가원 식품위해평가부 잔류물질과) | So-Young Cheon (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)
• 정지윤(식품의약품안전처 식품의약품안전평가원 식품위해평가부 잔류물질과) | Jiyoon Jeong (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)
• 조병훈(식품의약품안전처 식품의약품안전평가원 식품위해평가부 잔류물질과) | Byung-Hoon Cho (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)
• 이강봉(식품의약품안전처 식품의약품안전평가원 식품위해평가부 잔류물질과) | Kang-Bong Lee (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)