The present study was carried out to investigate the acute oral toxicity of Chamaecyparis obtusa (C. obtusa) essential oil in ICR male and female mice. Acute oral treatment with C. obtusa essential oil did not reveal any sign of toxicity or mortality in treated mice. Mouse body weights were not affected after single oral administration of C. obtusa essential oil during the 14-day observation period. In the hematological and blood biochemical analysis, all parameters of the treated group with 2,000 mg/kg body weight of the essential oil were not significantly different those of the control group. Therefore, the lethal dose 50 of the essential oil was estimated to be greater than 2,000 mg/ kg body weight in mice, which indicated that the essential oil is non-toxic. In conclusion, this study suggests that C. obtusa essential oil orally safe ICR mice.

ABSTRACT
 Materials and Methods
  공시제제
  실험동물
  시험물질 투여
  일반 중독증상 및 치사 동물수 관찰
  체중 측정
  혈액학적·혈액생화학적 분석
  부검 및 LD50산출
  통계학적 분석
 Results
  일반 중독증상 및 치사 동물수
  체중 변화
  혈액학적·혈액생화학적 분석
  부검소견 및 LD50값
 Discussion
 국문요약
 References

• 임창우(경상대학교 보건대학원, 포스코 광양제철소) | Chang-Woo Lim (Department of Environmental Health, Graduate School of Public Health, Gyeongsang National University, POSCO Gwangyang Works)
• 손송이(경상대학교 수의과대학·동물의학연구소) | Song Ee Son (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
• 이후장(경상대학교 보건대학원, 경상대학교 수의과대학·동물의학연구소) | Hu Jang Lee (Department of Environmental Health, Graduate School of Public Health, Gyeongsang National University, Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) Correspondence to