The purpose of this study was to develop an indirect enzyme-linked immunosorbent assay (indirect ELISA) based on a monoclonal antibody (MAb) that is specific to mackerel thermal stable-soluble protein (TSSP), that can be used for the rapid detection of mackerel in processed marine foods. Among the four MAbs (3A5-1, 2, 9, and 12) developed in previous studies, the 3A5-2 MAb that showed high specificity and sensitivity were selected and used to develop the indirect ELISA method. The detection range of the indirect ELISA was 0.02%-0.001% and the detection limit of 0.001% was shown. No cross-reaction to other marine products and food ingredients was observed by the indirect ELISA. Processed marine foods containing mackerel with ≥ 0.3 O.D. value at 405 nm were estimated as positive samples by the indirect ELISA. Therefore, the indirect ELISA can be used as a rapid and sensitive method to identify mackerel authenticity and adulteration in processed marine foods.

ABSTRACT
 Materials and Methods
  시료 및 재료
  단크론성 항체 생산
  간접효소면역분석법(indirect enzyme-linked immunosorbentassay; indirect ELISA)의 개발
  수산가공식품 중 고등어 검출
 Results and Discussion
  단크론성 항체 선택
  Indirect ELISA법의 최적화
  개발된 indirect ELISA법의 평가
  수산 가공 식품 중 고등어 어육 검출
 국문요약
 References

• 이정은(경상대학교 응용생명과학부) | Jeong-Eun Lee (Division of Applied Life Science, Graduate School, Gyeongsang National University)
• 김아윤(경상대학교 응용생명과학부) | Ah-Yoon Kim (Division of Applied Life Science, Graduate School, Gyeongsang National University)
• 김솔아(경상대학교 응용생명과학부) | Sol-A Kim (Division of Applied Life Science, Graduate School, Gyeongsang National University)
• 김효인(경상대학교 응용생명과학부) | Hyo-In Kim (Division of Applied Life Science, Graduate School, Gyeongsang National University)
• 박지혜(경상대학교 응용생명과학부) | Ji-Hye Park (Division of Applied Life Science, Graduate School, Gyeongsang National University)
• 심원보(경상대학교 농업생명과학연구원, 경상대학교 농화학식품공학과) | Won-Bo Shim (Institute of Agriculture and Life Science, Gyeongsang National University, Department of Agricultural Chemistry and Food Science & Technology, Gyeongsang National University) Correspondence to