This test was performed to evaluate the bactericidal efficacy of a fumigation disinfectant containing 20% ortho-phenylphenol against Pseudomonas aeruginosa (P. aeruginosa) and Enterococcus hirae (E. hirae). In preliminary tests, P. aeruginosa and E. hirae working culture suspension number (N value) were 2.8 × 108 and 4.0 × 108CFU/mL, respectively. And all the colony numbers on the carriers exposed to the fumigant (n1, n2, n3) were higher than 0.5N1 (the number of bacterial test suspentions by pour plate method), 0.5N2 (the number of bacterial test suspentions by filter membrane method) and 0.5N1, respectively. In addition, the mean number of P. aeruginosa and E. hirae recovered on the control-carriers (T value) was 2.8 × 108 and 3.4 × 106 CFU/mL, respectively. In the bactericidal effect of the fumigant, the reduction number of 2.8 × 108 (d value) was 6.46 and 5.19 logCFU/mL, respectively. According to the French standard for the fumigant, the d value for the effective bactericidal fumigant should be over than 5 logCFU/mL. With the results from this study, the fumigation disinfectant containing 20% ortho-phenylphenol has an effective bactericidal activity, then the fumigant can be applied to disinfect food materials and kitchen appliances contaminated with the pathogenic bacteria.

ABSTRACT
 재료 및 방법
  실험물질
  균주 배양
  환원유 혼합 현탁액의 균수계산
  균주의 담체 도포 및 소독제 적용
  소독제 잔류효과
  훈증소독제 적용
  실험 결과의 계산
  훈증소독제 살균효과 판정
 결과 및 고찰
  소독제 잔류효과
  훈증소독제의 살균효과
 요 약
 참고문헌

• 차춘남(경상대학교 공과대학 공학연구원) | Chun-Nam Cha (Research Institute of Life Sciences, College of Veterinary Medicine, Gyeongsang National University)
• 박은기(고신대학교 의과대학) | Eun-Kee Park (Department of Medical Humanities and Social Medicine, College of Medicine, Kosin University)
• 김용팔(엘캄코 바이오(주)) | Yongpal Kim (Elkahmco Bio Co., Ltd.)
• 유은아(보건복지부 통영검역소) | Eun-Ah Yu (Tongyeong National Quarantine Station, Ministry of Health & Welfare)
• 유창열(경남도립남해대학 인터넷정보학과) | Chang-Yeol Yoo (Department of Computer Information, Gyeongnam Provincial Namhae College)
• 홍일화(경상대학교 수의과대학 생명과학연구원) | Il-Hwa Hong (Research Institute of Life Sciences, College of Veterinary Medicine, Gyeongsang National University)
• 김 석(경상대학교 수의과대학 생명과학연구원) | Suk Kim (Research Institute of Life Sciences, College of Veterinary Medicine, Gyeongsang National University)
• 이후장(경상대학교 수의과대학 생명과학연구원) | Hu-Jang Lee (Research Institute of Life Sciences, College of Veterinary Medicine, Gyeongsang National University) Correspondence to