We have screened Bacillus strains suitable for the fermentation of soybean products with respect to the control of Bacillus cereus and the reduction of biogenic amines. Of 26 isolates, a strain named as the SCK A08 carried antimicrobial activity against B. cereus and Staphylococcus aureus, major food poisoning species in soybean products. PCR analysis revealed that the SCK A08 strain did not contain genes for Bacillus cereus toxins including nonhemolytic enterotoxin, hemolytic enterotoxin, cytotoxin K, cereulide and certrax. The SCK A08 strain could degrade histamine, tyramine, putrescine, and cadaverine by 67.41%, 76.59%, 57.32%, and 50.69%, respectively, during fermentation in cooked soybeans containing 0.5% (w/w) of each biogenic amine. The morphological and biochemical properties and phylogenetic relationships based on 16S rRNA gene sequences indicated that the isolate was most closely related to Bacillus licheniformis. Use of the strain SCK A08 would be a potential measure to overcome two hygienic problems that were frequently faced during manufacture of traditionally fermented soybean products.

ABSTRACT
 재료 및 방법
  균주의 선발 및 배양
  Biogenic amine 분석
  균주의 동정
  독소특성분석
  항균활성
  효소활성
  일반성분
  pH, 적정산도, 염도
  아미노태질소(AN) 함량
  청국장 제조
  관능검사
 결 과
  균주의 선발 및 동정
  독소특성 분석
  Biogenic amine 분석
  항균활성
  효소활성
  청국장의 품질특성
 요약 및 고찰
 참고문헌

• 이은실(순창군발효미생물관리센터) | Eon Sil Lee (Sunchang Research Center for Fermentation Microbes (SRCM))
• 김용상(전북대학교 자연과학대학 생명과학과) | Yong Sang Kim (Department of Biological Sciences, Chonbuk National University)
• 류명선(전북대학교 자연과학대학 생명과학과) | Myeong Seon Ryu (Department of Biological Sciences, Chonbuk National University)
• 정도연(순창군발효미생물관리센터) | Do Yeon Jeong (Sunchang Research Center for Fermentation Microbes (SRCM))
• 엄태붕(전북대학교 자연과학대학 생명과학과, 전북대학교 방사선 기술연구소) | Tai Boong Uhm (Department of Biological Sciences, Chonbuk National University, Institute for Radiation Technology, Chonbuk National University)
• 조성호(순창군발효미생물관리센터) | Sung Ho Cho (Sunchang Research Center for Fermentation Microbes (SRCM)) Correspondence to