This study aimed to investigate the changes in the γ-aminobutyric acid (GABA) content of bitter melon (Momordica charantia L.) cultivated from different regions, with different harvest times and at various maturation stages. Methods for observing the changes in GABA content were validated by determining the specificity, linearity, limit of detection (LOD), limit of quantification (LOQ), and precision and accuracy using the HPLC-FLD system. Results showed high linearity in the calibration curve with a coefficient of correlation (R2) of 0.9999. The LOD and LOQ values for GABA were 0.29 and 0.87 μg/mL, respectively. The relative standard deviations for intra- and inter-day precision of GABA were less than 5%. The recovery rate of GABA was in the range of 98.77% to 100.50%. The average content of GABA was 0.93 mg/g and Cheongju showed highest GABA content of 1.88 mg/g. As the time of harvest increased from May to September, the GABA content decreased from 1.56 to 0.86 mg/g. Also, maturation of the bitter melon fruit was associated with a decreased in GABA content.

Abstract
 서 론
 재료 및 방법
  1. 재료 및 시약
  2. 여주 추출물 제조
  3. GABA의 유도체화 및 HPLC 분석
  4. 분석법 검증(Method validation)
  5. 통계처리
 결과 및 고찰
  1. GABA 분석법 검증
  2. 재배지역별 GABA 함량
  3. 수확시기별 및 숙기별 GABA 함량
 요약 및 결론
 References

• 황인국(농촌진흥청 국립농업과학원 농식품자원부) | In Guk Hwang Corresponding author
• 이상훈(농촌진흥청 국립농업과학원 농식품자원부) | Sang Hoon Lee,
• 정윤숙(농촌진흥청 국립농업과학원 농식품자원부) | Yun Sook Jeong
• 송진(농촌진흥청 국립농업과학원 농식품자원부) | Jin Song
• 황경아(농촌진흥청 국립농업과학원 농식품자원부) | Kyung-A Hwang
• 조수묵(농촌진흥청 국립농업과학원 농식품자원부) | Soo Muk Ch