In order to understand biological phenomena accurately, single molecule techniques using a physical research approach to molecular interactions have been developed, and are now widely being used to study complex biological processes. In this review, we discuss some of the single molecule methods which are composed of two major parts: single molecule spectroscopy and manipulation. In particular, we explain how these techniques work and introduce the current research which uses them. Finally, we present the oral biology research using the single molecule methods.

서 론
 단분자 분광학(Single Molecule Spectroscopy)
  단분자 프렛(Single Molecule Förster Resonance Energy Transfer: smFRET) 기법
  단분자 추적기법 (Single Molecule Tracking)
  초고해상도 현미경(Super-resolution Microscopy)
 단분자 제어기술(Single Molecule Manipulation)
  광학 집게 (Oprical Tweezer)
  원자힘 현미경(Atomic Force Microscopy: AFM)
 구강 생물학 분야에서의 단분자 연구기법활용
 결 론
 References

• Jeong Hee Kim(Department of Oral Biochemistry and Molecular Biology, Kyung Hee University, KHU-KIST Department of Converging Science and Technology, Kyung Hee University)
• Cherlhyun Jeong(KHU-KIST Department of Converging Science and Technology, Kyung Hee University, Center for Theragnosis, Korea Institute of Science and Technology) Correspondence to