논문 상세보기

Anti-inflammatory activity of doxorubicin on lipopolysaccharide-induced hyperactivation of spleen cells KCI 등재

  • 언어KOR
  • URLhttps://db.koreascholar.com/Article/Detail/351530
구독 기관 인증 시 무료 이용이 가능합니다. 4,000원
예방수의학회지 (Journal of Preventive Veterinary Medicine)
한국예방수의학회(구 한국수의공중보건학회) (The Korean Society of Preventive Veterinary Medicine)
초록

Doxorubicin has been used to treating cancers, including breast cancer, bladder cancer, and acute lymphocytic leukemia, however, few studies have investigated its anti-inflammatory activity. In this study, we used mouse spleen cells treated with lipopolysaccharide (LPS), a representative inflammatory agent to investigate the effects of doxorubicin. Specially, we investigated the effects of doxorubicin on metabolic activity, cell size, cell death, and cytokine production of LPS-treated spleen cells. Doxorubicin significantly decreased the metabolic activity, even when applied at relatively low concentrations (1.6-8 ng/mL). To investigate the potential mechanism, we measured the mitochondrial membrane potential (MMP) of the LPS-treated spleen cells using Rhodamine 123. Doxorubicin decreased MMP and cell size, and induced cell death. Furthermore, doxorubicin suppressed the production of tumor necrosis factor (TNF)-alpha, a representative cytokine, in LPS-treated spleen cells. Taken together, doxorubicin decreased metabolic activity and the production of inflammatory cytokines, while increasing the death of LPS-induced hyperactivated spleen cells. This results will enable broader application of doxorubicin, as an anti-inflammatory agent, in clinical and research fields.

목차
Abstract
 서 론
 재료 및 방법
  실험동물과 시약
  비장세포의 분리와 물질처리
  비장세포의 대사활성도 측정
  유세포분석
  Enzyme-linked immunosorbent assay (ELISA)
  통계분석
 결 과
  LPS가 처리된 비장세포에서 독소루비신에 의한 대사활성 감소 효과.
  유세포분석을 통한 독소루비신이 처리된 비장세포의크기 변화 측정
  비장세포에서 독소루비신의 미토콘드리아 막전위 및세포사에 대한 영향
  독소루비신에 의한 비장세포의 염증성싸이토카인 생산 변화
 고 찰
 REFERENCES
저자
  • Ye-Ryoung Jang(Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Jeju National University)
  • Hye-Rin Yoon(Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Jeju National University)
  • Mi-Hyoung Kim(Department of Companion animal, Digital Seoul Culture Arts University)
  • Hong-Gu Joo(Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Jeju National University) Corresponding Author