Doxorubicin has been used to treating cancers, including breast cancer, bladder cancer, and acute lymphocytic leukemia, however, few studies have investigated its anti-inflammatory activity. In this study, we used mouse spleen cells treated with lipopolysaccharide (LPS), a representative inflammatory agent to investigate the effects of doxorubicin. Specially, we investigated the effects of doxorubicin on metabolic activity, cell size, cell death, and cytokine production of LPS-treated spleen cells. Doxorubicin significantly decreased the metabolic activity, even when applied at relatively low concentrations (1.6-8 ng/mL). To investigate the potential mechanism, we measured the mitochondrial membrane potential (MMP) of the LPS-treated spleen cells using Rhodamine 123. Doxorubicin decreased MMP and cell size, and induced cell death. Furthermore, doxorubicin suppressed the production of tumor necrosis factor (TNF)-alpha, a representative cytokine, in LPS-treated spleen cells. Taken together, doxorubicin decreased metabolic activity and the production of inflammatory cytokines, while increasing the death of LPS-induced hyperactivated spleen cells. This results will enable broader application of doxorubicin, as an anti-inflammatory agent, in clinical and research fields.

Abstract
 서 론
 재료 및 방법
  실험동물과 시약
  비장세포의 분리와 물질처리
  비장세포의 대사활성도 측정
  유세포분석
  Enzyme-linked immunosorbent assay (ELISA)
  통계분석
 결 과
  LPS가 처리된 비장세포에서 독소루비신에 의한 대사활성 감소 효과.
  유세포분석을 통한 독소루비신이 처리된 비장세포의크기 변화 측정
  비장세포에서 독소루비신의 미토콘드리아 막전위 및세포사에 대한 영향
  독소루비신에 의한 비장세포의 염증성싸이토카인 생산 변화
 고 찰
 REFERENCES

• Ye-Ryoung Jang(Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Jeju National University)
• Hye-Rin Yoon(Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Jeju National University)
• Mi-Hyoung Kim(Department of Companion animal, Digital Seoul Culture Arts University)
• Hong-Gu Joo(Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Jeju National University) Corresponding Author