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Naturally produced honey by feeding chitosan to the honeybees KCI 등재
- 언어KOR
- URLhttps://db.koreascholar.com/Article/Detail/351534
pp.80-85
Chitosan is attracting attention as a health supplement material because of its various physiological activities. In this study, sugar solution containing chitosan was fed to honey bees to induce the production of ‘chitosan fortified honey’ by their same mode of natural honey production. To accomplish this, sugar solutions containing 0.1%, 1%, 2% and 5% chitosan were fed to the honey bees. Fully inverted Chitosan-honey was harvested after feeding the chitosan in sugar solution. To investigate the anti-obesity and immune-enhancing effects of Chitosan-honey, 1% and 10% Chitosan-honey containing drinking water were administrated freely to C57BL mice. Glucosamine concentrations in serum rapidly increased to peak levels in 10 minutes (1261.0 ± 97.6 ng/ml), then decreased gradually for more than 24 hours 793.0 ± 34.7 ng/ml. There were no significant differences in weight and or splenocyte proliferative capacity among experimental mice groups. However, increased granulocytes and monocytes were observed upon flow cytometric analysis. These results suggest that Chitosan-honey could induce removal of foreign antigens. In conclusion, ‘Chitosan-honey’ developed in this study has the potential for use as a honey type dietary health supplement with the same bioactivity as chitosan; however additional research should be conducted to confirm these effects.
서 론
재료 및 방법
키토산
키토산벌꿀의 생산
키토산벌꿀에서의 키토산 함유지표 측정 (총 글루코사민)
당함량 분석
키토산벌꿀의 체중증가억제 효과
마우스 혈액 중 총 글루코사민 함량 변화
키토산벌꿀 투여에 따른 비장세포 증식능
비장세포의 유세포 분석
통계처리
결과 및 고찰
키토산벌꿀의 생산
키토산벌꿀의 성상변화
마우스에서의 비만억제 효과
혈액 중 총 글루코사민 함량 변동분석
비장세포의 증식능
비장세포의 유세포 분석(Flow cytometry)을 통한 면역활성능 측정
키토산벌꿀의 상품화 전망
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