The cryopreservation has been extensively applied in many cells including spermatozoa (semen) during past several decades. Especially, the canine spermatozoa cryopreservation has contributed on generation of progeny of rare/genetically valuable dog breeds, genome resource banking and transportation of male germplasm at a distant place. However, severe and irreversible damages to the spermatozoa during cryopreservation procedures such as the thermal shock (cold shock), formation of intracellular ice crystals, osmotic shock, stress of cryoprotectants and generator of reactive oxygen species (ROS) have been addressed. According as a number of researches have been conducted to overcome these problems and to advance cryopreservation technique, several analytical methods have been employed to evaluate the quality of the fresh or cryopreserved canine spermatozoa in regards to the motility, morphology, integrity of membrane and DNA, mitochondrial activity, ROS generation, binding affinity to oocytes, in vitro fertilization potential and fertility potential by artificial insemination. Because the study designs with certain application of analytical methods are selective and varied depending on each experimental objective and laboratory condition, it is necessary to establish the normal reference data of the fresh or cryopreserved canine spermatozoa for each analytical method to monitor experimental procedure, to translate raw data and to discuss results. Here, we reviewed the recent articles to introduce various analytical methods for the canine spermatozoa as well as to establish the normal reference data for each analytical method in the fresh or cryopreserved canine spermatozoa, based on the results of the previous articles. We hope that this review contributes to the advancement of cryobiology in canine spermatozoa.

Abstract
 INTRODUCTION
 The normal reference data in the analysis of the fresh or cryopreserved canine spermatozoa
 CONCLUSION
 DISCLOSURE STATEMENT
 REFERENCES

• Byung-Joon Park(College of Veterinary Medicine, Kyungpook National University)
• Seung-Joon Kim(College of Veterinary Medicine, Kyungpook National University)
• Hyeon-Jeong Lee(College of Veterinary Medicine, Gyeongsang National University)
• Sung-Lim Lee(College of Veterinary Medicine, Gyeongsang National University)
• Gyu-Jin Rho(College of Veterinary Medicine, Gyeongsang National University)
• Won-Jae Lee(College of Veterinary Medicine, Kyungpook National University) Correspondence